CAZyme Information: MGYG000004891_02334

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; KM106-2
• CAZyme ID: MGYG000004891_02334
• CAZy Family: GH28
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1299	MGYG000004891_31|CGC1	141077.06	9.22

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004891	3652874	MAG	China	Asia

• Gene Location: Start: 18354; End: 22253 Strand: +

Full Sequence      

MKKNMARFTALATAAAIACSTFFSPVGTIAYAQSFTSSEGSYNVTGLKVPTLAYDDESIT
LVWDKPENYSSVADYNIYINGEKKGTARESFRTNADWAATYMDAFYAGNEKKNVDMVNVD
IHSYKATGLTPSTEYTFEVRAVDATGKELGTSNVIKQSTTAKEQEFNIVDYGAKTSEGYT
SYNDEINAFIEANTKAIQSAIDACTEGGKVVIPEGTFVSGAIYLKSNMTLELQEGAVLWG
SPNTDHYDHNYLLYPYSTDTRAWALVNAYSADENQMYENIRITGQGTIYGNGWKYGATNT
QYGDGISEPLYQDQTALDPDYTELPQYRLERWMAGKAANAISQGILAKDAYEKAFAELNN
STKAYATRPNLVALRGVDGIYIEGITVKNPAFHTIALLDSKNVVSTDVKYITYDCNNADG
IEIGNTQNALIFNNFFDTGDDSINFATGLGNGVNDSEQQASSNIWTFNNFIRNGHGGAIA
AGSHTGAGIGNMLVEDNVINLNEMPFRFKSAPVNGGGIYNVTIRDCAVANCKQLFVMTTS
YGDANQAFEVEPADKPAEFSNISAYNITADTISKNSFYLEADVSAVYKPWHTHHDLYFQD
ITCTNITTKTSEVIKGCENVEFNNVSLNWNASKVATPTPWSTIQNSKGISFTGTTTTSAV
AEDAMQAPEWPDGVKVSVTSDVVANTGYNTADVTLTWNEAENATKYSIDVLVDGEKVDQL
DGITDTTKTITGLSTGVEYTYKVYACDATGNKTLGRTVTTKSAGKKDVTPVVGPTDSALT
LDGTGYTWTRATFTKASLTDSRVRGYKAYVNGTLKETFYNYKIKDGTNPDLISQTVRRLT
PGTENTIKIVAFTDAGCEFVYEEGKVTTPKNYDHKAPVWNNGSKLTVKTEGNDIVLSWPA
ATDDTAVNGYRVYVDGKPVLGTDNKTFNQVNGYYTTNKTSYKVSGLDLTKEHTFKVEAGD
TWWKAAEGDGPYHWTLSGPTAVYKPVVQKPEESKPTETTKPTATPTPTVTTVAQVKNLKV
SAVNTTSVKATWSKADANGYVVSYRNAAGKTVTKTITSASTTSCVLTGLAPNRSVTVTIK
AYKTVNKKPVYSKTVSASGKTKALAKVTSVKASSVKNDSFKVSFKKVSEADGYVVTYKNA
LKKTTTVTLKGNAKTSLTISNLLSNKTYSVAIKPYKVVNGKKVYGSTVTKSIKTAKTVKP
AKVTLNKVTKKSKTSAKVSFKSAKNAAGYEVSYSLKKNSGYKKVTVKKASTVTLSKLKSK
GTYYVKVRAYTYENKVVKGKVVKQKVYGATSTIKKVSLR

Enzyme Prediction     

No EC number prediction in MGYG000004891_02334.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	203	621	5.6e-53	0.9661538461538461

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5434	Pgu1	4.62e-63	49	574	3	437	Polygalacturonase [Carbohydrate transport and metabolism].
pfam00295	Glyco_hydro_28	1.99e-08	367	627	83	303	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
pfam00041	fn3	1.38e-07	1014	1089	2	83	Fibronectin type III domain.
cd00063	FN3	1.17e-06	1015	1082	4	77	Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.
smart00060	FN3	1.93e-06	1015	1082	4	77	Fibronectin type 3 domain. One of three types of internal repeat within the plasma protein, fibronectin. The tenth fibronectin type III repeat contains a RGD cell recognition sequence in a flexible loop between 2 strands. Type III modules are present in both extracellular and intracellular proteins.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SET92437.1	0.0	35	979	96	1061
QOV20136.1	1.44e-274	35	999	65	1090
AIF50363.1	2.09e-163	47	980	42	898
AJQ29053.1	2.34e-155	47	980	41	901
AOV08612.1	1.30e-150	37	936	28	902

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2UVE_A	4.55e-116	47	640	41	591	Structureof Yersinia enterocolitica Family 28 Exopolygalacturonase [Yersinia enterocolitica],2UVE_B Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase [Yersinia enterocolitica],2UVF_A Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase in Complex with Digalaturonic Acid [Yersinia enterocolitica],2UVF_B Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase in Complex with Digalaturonic Acid [Yersinia enterocolitica]
3JUR_A	1.12e-12	163	564	25	383	Thecrystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_B The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_C The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_D The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima]
5OLP_A	1.88e-11	163	539	42	368	Galacturonidase[Bacteroides thetaiotaomicron VPI-5482],5OLP_B Galacturonidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P15922	5.27e-110	47	645	36	592	Exo-poly-alpha-D-galacturonosidase OS=Dickeya chrysanthemi OX=556 GN=pehX PE=1 SV=1
P58598	5.13e-10	191	544	72	404	Polygalacturonase OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=pglA PE=3 SV=1
P20041	1.54e-09	191	541	70	399	Polygalacturonase OS=Ralstonia solanacearum OX=305 GN=pglA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001110	0.759669	0.237803	0.000788	0.000343	0.000270

TMHMM  Annotations     

start	end
7	29