CAZyme Information: MGYG000004899_03823

Basic Information

• Species: Bacteroides sp900555635
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp900555635
• CAZyme ID: MGYG000004899_03823
• CAZy Family: PL8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1562	MGYG000004899_32|CGC1	176681.55	7.6685

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004899	5396709	MAG	China	Asia

• Gene Location: Start: 146; End: 4834 Strand: -

Full Sequence      

MRRLQVLVFILCLLSPWAAAGEVKTDDQKELDEITRRLISTHLEAEVDDEAIARYIKLIR
PDGSFPDLNYVATYDGAYFPCGTHLKRLDSMAVAYRKPDSKFHDSSKLLNKIVAGLDYWY
KERPVSKNWWFNEIGAPQDYMVPLILLKGKISDKKLLHYSSFLKDLTWNKGHKGKNRTWV
SSVTIHKGCIENNIELVRIGFKSIASTIQIVSRQGDEGIKIDGSFHQHRPQLYSGGYGLS
YVDDIAYYLQLVRGTAFEPYFTQEKKNIFIDLLLNGHRWLGYRETFDFGAVGRNISRSKG
VSNVSATVLERMEQSDPSHAMDYVAWRKHLSGAAFPVPGNKYFWKSDFMVQHGTNYYLSA
KVISARTNGTEMLNNENLKGYNLPLGATNILTSGKEYKSIFPVWEWTRIPGTTAVQHPDS
ARLDGYHFGKNDFGGGVSNGKSGMIAYQHDYKGVKANKAYFFLEDVLVCLGAGITSDALQ
EVATTVNQCYFSGNMVVSKENLVTTYKENESVKNPAWVYHDRVGYLFPSGGEITAGSKQQ
TGSWKDINLSESGKEISADIFNLWISHGKKATNGKYAYMIVPDKSLDEFQKFTTTQTIYI
VKNTPEVQAVKSRHMYAVVFYHSGKVELESGLTLSADRPVMVYVEQQSGKFKIWAADPLF
SQKRVYLTLNGREVCIDFPKDAFMGSTASVEIADSQPYDLQCEYLQNPLGLDVLQPRLSW
KLGTTSLARGLKQTAYQVLVSSSQALLDQDSGDLWNSGTICSSESVNITYVGTPLSSGKA
CYWKVRFQDAQGVWSSWSAPAFWRMGLLADDWAARWIGSAEMEMQSVGGKRVDNKMQDPW
LRKTFTLADAPQDAIMYVASIGYHELYVNGQKVGDAVLSPSVTDHKSRARYMTYDIADYL
KPGKNVVALWLGTSWAVFSVYQREDKPAIPMALAQLEISLSSGKKLQIISDETWKVHASP
NMLTGYWEAHHFAGEFYDAALEIDGWNEASFNDSGWGAAKVYDPSIQVSADRTEPNRLVE
EIKPLSIQEVSPGIYRVDMGINYTGWFEMQLEGLPGDSILFQFSEREKDVCSYGIQSTYK
VGPKRKGVFCNRFNYMTGRWVQISGLRRKPALEQIRGWMIRPDYRRTGGFECDIPLLNDI
YRTTLWTFENLSLGNYVVDCPHRERCGYGGDALATTRTAMGNYQLGAFYNKWMEDWRDVQ
DEEGNVPYTAPTRIGGGGPSWSGFCITLPWEFYRQYGDVRILSESFPTIQRWLDFVETKS
ENDMLTRWGGKWSFLGDWLWPNVWPERSALEKQGKALGDTRETLFFNNCHWIYSLETAAQ
IADVLGHKTVAMTYRKRASQIRKAVHATFFNSADNSYVNGFPSYLAIAIMVDLPPKPLKA
KVWKRLEQEISVNRKGHIWGGITAGSFLMHTLLDNHRNDLIFEMAMKEDFPGWGNMLKLG
SGTLFEDWECRGSALHSSYLYIGSWFIEALGGIRRPEAGYNQFVIAPWITKDGPKQVRAH
YNSMYGDIVSNWSVKSDQLQIEVVIPANSTAVLKLPDVLPGPLSLTLQAGTYNFSFKMNW
NK

Enzyme Prediction     

No EC number prediction in MGYG000004899_03823.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	1027	1535	2.5e-121	0.998015873015873
PL8	341	583	1e-82	0.9879518072289156
CBM67	814	1004	1.5e-36	0.9602272727272727

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01083	GAG_Lyase	1.19e-126	82	658	64	693	Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
pfam02278	Lyase_8	2.52e-89	340	585	1	252	Polysaccharide lyase family 8, super-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.
pfam17389	Bac_rhamnosid6H	1.55e-72	1125	1470	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	6.66e-44	850	1020	2	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam08124	Lyase_8_N	3.86e-31	83	312	60	302	Polysaccharide lyase family 8, N terminal alpha-helical domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT88602.1	0.0	1	1559	1	1583
ALJ60391.1	0.0	1	1559	3	1585
QUT74996.1	0.0	696	1559	34	920
BCI64727.1	0.0	690	1538	33	881
QDU88571.1	2.90e-288	681	1536	779	1645

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6I60_A	3.39e-111	679	1536	16	899	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
1CB8_A	1.34e-97	49	691	25	678	CHONDROITINASEAC LYASE FROM FLAVOBACTERIUM HEPARINUM [Pedobacter heparinus]
1HM2_A	2.33e-97	49	691	47	700	ACTIVESITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HM3_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-Oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus],1HMU_A ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HMW_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus]
3W5M_A	8.18e-92	703	1536	12	995	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6GSZ_A	4.43e-73	703	1536	10	847	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KNB2	1.73e-102	703	1536	42	879	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
Q59288	1.27e-96	49	691	47	700	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=cslA PE=1 SV=1
T2KPL4	1.16e-92	707	1535	42	906	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
Q82PP4	3.65e-91	703	1536	12	995	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
P9WF03	4.38e-83	692	1531	26	874	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000212	0.999217	0.000163	0.000145	0.000140	0.000130

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004899_03823.