Basic Information | |
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Species | Arabidopsis thaliana |
Cazyme ID | AT4G27550.1 |
Family | GT20 |
Protein Properties | Length: 796 Molecular Weight: 89474.4 Isoelectric Point: 6.5101 |
Chromosome | Chromosome/Scaffold: 4 Start: 13755649 End: 13759740 |
Description | trehalose-6-phosphate synthase, putative, expressed |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 4 | 466 | 0 |
PRLLVVSMSLPVTAKRTGEESWSFTMSPGGLVSALLGLKEFETKWIGWPGVDVHDAIGKKTLSITLAEKGCIPVFLEEVCDQYYNGYCNNILWPIFHYLG TPPEYRNDATITYQSQYEAYKKANQIFFDVVKEHYEEGDVVWCHDYHVMLLPQYLKEYNSKMKVGWFLHTPFPSSEMYKTLPSRSDLLRSVLTADLVGFH TYDFARHFLNACMCILGVEATSEGIVDQGKVTRVAVFPIGIEPERFINTSELSEVVQYMKKFKNDFGGRKLILGVDRLDTIKGIPQKYQAFEKFLEENAE WRGKVMLLQIAVPTRNGIGEYQKIKDQCHYHVGRINGRFGSISSVPIIHLDCSIDFNQLCALYAITDVLLVTSLRDGMNLVSSEFIACQKAEKGVLILSE FAGAGQSLGAGAILVNPWNIKEVSSAIGEALNMSHEEKERKHKINFQYVKTHSTQQWADDFMK |
Full Sequence |
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Protein Sequence Length: 796 Download |
MARPRLLVVS MSLPVTAKRT GEESWSFTMS PGGLVSALLG LKEFETKWIG WPGVDVHDAI 60 GKKTLSITLA EKGCIPVFLE EVCDQYYNGY CNNILWPIFH YLGTPPEYRN DATITYQSQY 120 EAYKKANQIF FDVVKEHYEE GDVVWCHDYH VMLLPQYLKE YNSKMKVGWF LHTPFPSSEM 180 YKTLPSRSDL LRSVLTADLV GFHTYDFARH FLNACMCILG VEATSEGIVD QGKVTRVAVF 240 PIGIEPERFI NTSELSEVVQ YMKKFKNDFG GRKLILGVDR LDTIKGIPQK YQAFEKFLEE 300 NAEWRGKVML LQIAVPTRNG IGEYQKIKDQ CHYHVGRING RFGSISSVPI IHLDCSIDFN 360 QLCALYAITD VLLVTSLRDG MNLVSSEFIA CQKAEKGVLI LSEFAGAGQS LGAGAILVNP 420 WNIKEVSSAI GEALNMSHEE KERKHKINFQ YVKTHSTQQW ADDFMKLTLT NILCSKLIEI 480 TTSAELGAGL AATLELPEHD VIQQYSKSNN RLLILGFYGT LTQPMKNQER RGDGMNLELH 540 PQLKERLKEL CSDPKTTVVV LSRSEKCILD KNFGEYNMWL AAENGMFLRH TSGEWVTRIP 600 EHMNLEWIDG VKHVFKYFTE RTPGSYLETS EASLVWNYEN ADAEFGRAQA RDMLQHLWAG 660 PISNASVDVV RGGQSVEVHA VGVTKGSAME RILGEIVHNK SMATPIDYVL CIGCFLGKDE 720 DVYTFFEPEL TKKAKSLSSS GSDSPKKVSS TIVDLKGENY FSVAIGQTHT KARYFLDSSD 780 DVVKLIGKLC THNNA* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN03064 | PLN03064 | 1.0e-8 | 752 | 789 | 38 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PLN03063 | PLN03063 | 0 | 1 | 795 | 795 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PLN03064 | PLN03064 | 0 | 2 | 730 | 733 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PRK14501 | PRK14501 | 0 | 5 | 789 | 796 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
TIGR02400 | trehalose_OtsA | 0 | 5 | 466 | 465 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0003825 | alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity |
GO:0005992 | trehalose biosynthetic process |
GO:0016757 | transferase activity, transferring glycosyl groups |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | NP_173142.1 | 0 | 3 | 790 | 10 | 771 | ATTPS2; alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | NP_177979.1 | 0 | 3 | 740 | 91 | 822 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | NP_177979.1 | 0.00000005 | 736 | 790 | 882 | 936 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | NP_194485.1 | 0 | 1 | 795 | 1 | 795 | ATTPS4; alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
Swiss-Prot | Q9FZ57 | 0 | 3 | 791 | 11 | 773 | TPS2_ARATH RecName: Full=Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2; AltName: Full=Trehalose-6-phosphate synthase 2; Short=AtTPS2 |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1gz5_D | 0 | 30 | 465 | 19 | 447 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_C | 0 | 30 | 465 | 19 | 447 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_B | 0 | 30 | 465 | 19 | 447 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 1gz5_A | 0 | 30 | 465 | 19 | 447 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
PDB | 2wtx_D | 0 | 30 | 465 | 20 | 448 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EG462462 | 243 | 1 | 243 | 0 |
ES813375 | 369 | 365 | 730 | 0 |
DW069910 | 288 | 90 | 377 | 0 |
CV711307 | 294 | 2 | 294 | 0 |
EG462462 | 25 | 232 | 256 | 0.00001 |
Sequence Alignments (This image is cropped. Click for full image.) |
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