APIS family ID | APIS001 | |||||||||||||
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Inhibited defense system | pyrimidine cyclase system for antiphage resistance (Pycsar) | |||||||||||||
CLAN ID | CLAN030 | |||||||||||||
Seed protein property |
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Phage property |
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PMID/References | PMID:35395152 | |||||||||||||
PDB structures | 7T28,Bsp38;7U2R,Paenibacillus J14;7U2S,Paenibacillus xerothermodurans; | |||||||||||||
Pfam domains | PF00753.30 | |||||||||||||
Phrog | phrog_392 | |||||||||||||
Host taxa | d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Bacillaceae;g__Bacillus;s__Bacillus subtilis | |||||||||||||
Gene Location | Start: 92525; End: 93295; Strand: + | |||||||||||||
Description | SBSphiJ Apyc1 efficiently hydrolyses a wide range of cyclic mononucleotides; 273 Acb1 and 107 Apyc1 phage proteins were identified. |
Genome accession | Start | End | Strand | Protein ID | Length | Molecular weight | Charge | Isoelectric point | Function | Phrog | PFAM |
---|---|---|---|---|---|---|---|---|---|---|---|
NC_048726.1 | 90711 | 90842 | + | YP_009840589.1 | 43 | 5432.17 | 1.5 | 7.3202 | hypothetical protein | ||
NC_048726.1 | 90832 | 91815 | + | YP_009840590.1 | 327 | 37645.45 | -14.5 | 4.4760 | ribonucleotide reductase class Ia beta subunit | phrog_10582 ; phrog_24856 ; phrog_7909 ; phrog_86 ; phrog_9465 ; phrog_9530 | PF00268.24 |
NC_048726.1 | 91815 | 92051 | + | YP_009840591.1 | 78 | 8693.89 | -7.5 | 4.0742 | putative thioredoxin | ||
NC_048726.1 | 92152 | 92349 | + | YP_009840592.1 | 65 | 7822.74 | -4.5 | 4.5778 | hypothetical protein | phrog_24464 | |
NC_048726.1 | 92346 | 92525 | + | YP_009840593.1 | 59 | 7041.92 | -14.5 | 3.3286 | hypothetical protein | ||
NC_048726.1 | 92525 | 93295 | + | YP_009840594.1 | 256 | 29051.70 | -9.5 | 4.8545 | metal-dependent hydrolase | phrog_392 | PF00753.30 |
NC_048726.1 | 93311 | 93502 | + | YP_009840595.1 | 63 | 6927.92 | -3.5 | 4.4895 | hypothetical protein | phrog_11210 | |
NC_048726.1 | 93502 | 93657 | + | YP_009840596.1 | 51 | 5795.81 | -4.5 | 4.2507 | hypothetical protein | phrog_28167 ; phrog_19268 ; phrog_21011 | |
NC_048726.1 | 93654 | 93848 | + | YP_009840597.1 | 64 | 7430.41 | -1.5 | 5.6149 | hypothetical protein | ||
NC_048726.1 | 93845 | 94063 | + | YP_009840598.1 | 72 | 8315.41 | -8.0 | 4.0078 | acetyltransferase | phrog_5504 | |
NC_048726.1 | 94066 | 94446 | + | YP_009840599.1 | 126 | 14832.61 | -12.0 | 4.1674 | hypothetical protein | phrog_17088 |
Hit | Query start | Query end | Hit start | Hit end | TMscore(ali) | TMscore(avg) |
---|---|---|---|---|---|---|
AF-A0A0H3GHL8-F1-model_v4 | 5 | 238 | 1 | 238 | 0.8284 | 0.7878 |
AF-A0A133CTF0-F1-model_v4 | 1 | 238 | 1 | 244 | 0.8176 | 0.7862 |
AF-P9WGC1-F1-model_v4 | 1 | 238 | 18 | 267 | 0.8368 | 0.7674 |
AF-Q58769-F1-model_v4 | 1 | 238 | 3 | 253 | 0.816 | 0.7554 |
AF-P50474-F1-model_v4 | 1 | 238 | 27 | 276 | 0.8371 | 0.7551 |
AF-P16692-F1-model_v4 | 1 | 238 | 3 | 249 | 0.7862 | 0.7487 |
AF-A0A0H3GM29-F1-model_v4 | 1 | 238 | 3 | 249 | 0.7863 | 0.7487 |
AF-Q329H1-F1-model_v4 | 1 | 238 | 3 | 249 | 0.7848 | 0.747 |
AF-O74545-F1-model_v4 | 1 | 238 | 6 | 297 | 0.8418 | 0.7433 |
AF-K0EUK8-F1-model_v4 | 22 | 238 | 1 | 230 | 0.7727 | 0.742 |
Hit | Query start | Query end | Hit start | Hit end | TMscore(ali) | TMscore(avg) |
---|---|---|---|---|---|---|
MGYP001101967902.pdb | 1 | 238 | 5 | 254 | 0.9231 | 0.8778 |
MGYP002654867125.pdb | 1 | 238 | 3 | 253 | 0.9006 | 0.8575 |
MGYP003952926961.pdb | 1 | 238 | 2 | 251 | 0.8769 | 0.8337 |
MGYP001424664394.pdb | 1 | 238 | 1 | 240 | 0.857 | 0.8335 |
MGYP000754846076.pdb | 1 | 238 | 3 | 243 | 0.8585 | 0.8314 |
MGYP000856702051.pdb | 1 | 238 | 3 | 265 | 0.8913 | 0.8294 |
MGYP001478949968.pdb | 1 | 238 | 1 | 258 | 0.8806 | 0.8291 |
MGYP001450167839.pdb | 1 | 238 | 1 | 245 | 0.8676 | 0.8291 |
MGYP000956383480.pdb | 1 | 238 | 1 | 240 | 0.853 | 0.8289 |
MGYP001465423269.pdb | 1 | 238 | 1 | 251 | 0.8711 | 0.8287 |
Fam ID | Seed protein | Member count | Model | Alignment | APIS001 | YP_009840594.1 | 26 | HMM model | Member alignment |
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Hit APIS family | E-Value | Query Start | Query End | Hit Start | Hit End | Immune system | Seed protein |
---|---|---|---|---|---|---|---|
APIS076 | 1.49E-66 | 17 | 271 | 3 | 259 | IMGVR_UViG_3300044693_000096|3300044693|Ga0466961_0000159_42271_43065 | |
APIS058 | 3.13E-56 | 18 | 271 | 14 | 268 | IMGVR_UViG_3300022562_000702|3300022562|Ga0247387_1000200120 | |
APIS062 | 2.02E-54 | 20 | 271 | 1 | 263 | IMGVR_UViG_3300028374_000006|3300028374|Ga0306906_100003345 | |
APIS073 | 6.60E-53 | 19 | 271 | 22 | 285 | IMGVR_UViG_3300042197_004918|3300042197|Ga0451494_0000046_37885_38742 | |
APIS054 | 1.35E-43 | 19 | 270 | 2 | 261 | IMGVR_UViG_3300014204_000482|3300014204|Ga0172381_1000105623 | |
APIS041 | 9.01E-43 | 19 | 271 | 1 | 249 | IMGVR_UViG_2832702071_000001|2832702071|2832704097 | |
APIS066 | 2.83E-40 | 20 | 271 | 1 | 293 | IMGVR_UViG_3300035396_000446|3300035396|Ga0393109_006065_59543_60412 | |
APIS060 | 2.90E-36 | 20 | 271 | 1 | 259 | IMGVR_UViG_3300024258_000005|3300024258|Ga0233440_1000037199 | |
APIS064 | 1.21E-35 | 20 | 271 | 1 | 258 | IMGVR_UViG_3300033145_003233|3300033145|Ga0366840_1000004654 | |
APIS044 | 7.63E-31 | 22 | 260 | 2 | 233 | HM144387_00160 |
Member | pident | evalue | bitscore | qcovs | phage | host | SourceDB | Pfam | Phrog |
---|---|---|---|---|---|---|---|---|---|
IMGVR_UViG_2974673286_000001|2974673286|2974673417 | 100.000 | 0.0 | 528 | 99% | IMGVR | PF00753 | phrog_392 | ||
IMGVR_UViG_3300027628_000019|3300027628|Ga0209463_100018292 | 50.973 | 3.06e-78 | 245 | 98% | IMGVR | PF12706 | phrog_392 | ||
IMGVR_UViG_3300049046_000017|3300049046|Ga0498743_000341_2770_3534 | 51.362 | 6.47e-77 | 241 | 98% | IMGVR | PF12706 | phrog_392 | ||
JN638751_00385 | 41.494 | 7.02e-41 | 149 | 90% | Bacillus phage G | Bacillus | INPHARED | PF12706 | phrog_392 |
KF669652_00121 | 87.938 | 1.32e-164 | 464 | 99% | Bacillus phage Grass | Bacillus | INPHARED | PF00753 | phrog_392 |
KM236245_00126 | 50.000 | 2.13e-78 | 245 | 97% | Bacillus phage Mater | Bacillus | INPHARED | PF12706 | phrog_392 |
KY368640_00118 | 78.846 | 2.48e-144 | 412 | 99% | Bacillus phage vB_BsuM-Goe3 | Bacillus | INPHARED | PF00753 | phrog_392 |
LT960608_00153 | 87.549 | 4.12e-163 | 460 | 99% | Bacillus phage SBSphiJ | Bacillus | INPHARED | PF00753 | phrog_392 |
MH606185_00128 | 100.000 | 0.0 | 528 | 99% | Bacillus phage BSP38 | Bacillus | INPHARED | PF00753 | phrog_392 |
MT151604_00140 | 54.365 | 1.09e-79 | 248 | 98% | Bacillus phage P59 | Bacillus | INPHARED | PF00753 | phrog_392 |
MW419085_00133 | 66.403 | 4.86e-108 | 320 | 98% | Bacillus phage 000TH009 | Bacillus | INPHARED | PF00753 | phrog_392 |
MW419086_00121 | 65.613 | 2.56e-107 | 318 | 98% | Bacillus phage 015DV002 | Bacillus | INPHARED | PF00753 | phrog_392 |
MW419087_00140 | 66.008 | 6.12e-108 | 320 | 98% | Bacillus phage 015DV004 | Bacillus | INPHARED | PF12706 | phrog_392 |
MW749002_00188 | 65.217 | 2.42e-106 | 316 | 98% | Bacillus phage vB_BspH_Mawwa | Bacillus | INPHARED | PF00753 | phrog_392 |
MW749007_00191 | 65.217 | 2.60e-107 | 318 | 98% | Bacillus phage vB_BspH_TimeGriffin | Bacillus | INPHARED | PF00753 | phrog_392 |
MZ501263_00147 | 84.825 | 1.05e-157 | 446 | 99% | Bacillus phage 043JT007 | Bacillus | INPHARED | PF00753 | phrog_392 |
MZ501265_00143 | 84.825 | 1.21e-157 | 446 | 99% | Bacillus phage 278BB001 | Bacillus | INPHARED | PF00753 | phrog_392 |
OK349510_00018 | 77.821 | 2.65e-142 | 407 | 99% | Bacillus phage BM-P1 | Bacillus | INPHARED | PF00753 | phrog_392 |
OM728301_00121 | 78.210 | 2.35e-142 | 407 | 99% | Bacillus phage vB_BsuM-Goe20 | Bacillus | INPHARED | PF00753 | phrog_392 |
OM728302_00121 | 78.462 | 1.60e-143 | 410 | 99% | Bacillus phage vB_BsuM-Goe24 | Bacillus | INPHARED | PF00753 | phrog_392 |
OM728303_00122 | 79.377 | 1.05e-145 | 416 | 99% | Bacillus phage vB_BsuM-Goe25 | Bacillus | INPHARED | PF00753 | phrog_392 |
OM982669_00151 | 86.770 | 2.11e-159 | 451 | 99% | Bacillus phage SBSphiJ2 | Bacillus | INPHARED | PF00753 | phrog_392 |
OM982671_00150 | 85.992 | 7.13e-158 | 447 | 99% | Bacillus phage SBSphiJ4 | Bacillus | INPHARED | PF00753 | phrog_392 |
OM982673_00147 | 86.770 | 9.37e-160 | 451 | 99% | Bacillus phage SBSphiJ6 | Bacillus | INPHARED | PF00753 | phrog_392 |
OM982674_00148 | 87.160 | 4.16e-163 | 460 | 99% | Bacillus phage SBSphiJ7 | Bacillus | INPHARED | PF00753 | phrog_392 |
YP_009840594.1 | 100.000 | 0.0 | 528 | 100% | Bsp38 of Bacillus subtilis | Bacillus subtilis | NCBI | PF00753 | phrog_392 |
APIS | Contig | Range |
---|---|---|
APIS001 | NC_048726.1 | 90711 - 94446 |