dbAPIS: a database of anti-prokaryotic immune systems

Seed protein information help

APIS family ID

APIS092

Inhibited defense system

RecBCD

CLAN ID

CLAN021

Seed protein property

Accession ID	Gene name	Protein length	Molecular weight	Isoelectric point	Charge
NP_040618.1	gene_page: Gam	138	16346.3	5.7699	-3.5

Phage property

Phage	Assembly id
Lambda of E. coli	GCF_000840245.1

PMID/References

PMID:24086157

PDB structures

2UV1;

Pfam domains

PF06064.14

Phrog

phrog_259,phrog_26899

Host taxa

d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Escherichia;s__Escherichia coli

Gene Location

Start: 32816; End: 33232; Strand: -

Description

The protein Gam inhibits the host RecBCD exonuclease activity thus allowing efficient rolling-circle replication.

Seed genomic context Download table help

Genome accession	Start	End	Strand	Protein ID	Length	Molecular weight	Charge	Isoelectric point	Function	Phrog	PFAM
NC_001416.1	30839	31024	-	NP_040613.1	61	6738.56	5.0	8.7760	hypothetical protein
NC_001416.1	31005	31196	-	NP_040614.1	63	7023.04	-2.5	4.9938	DUF1382 family protein	phrog_292	PF07131.14
NC_001416.1	31169	31351	-	NP_040615.1	60	6754.93	7.5	11.1907	DUF1317 domain-containing protein	phrog_1998	PF07026.14
NC_001416.1	31348	32028	-	NP_040616.1	226	25908.58	-3.0	5.4127	exonuclease	phrog_434 ; phrog_149 ; phrog_38119 ; phrog_19459	PF09588.13
NC_001416.1	32025	32810	-	NP_040617.1	261	29688.64	-6.5	4.8663	RecT-like ssDNA annealing protein	phrog_12233 ; phrog_10056 ; phrog_17377 ; phrog_2449 ; phrog_252 ; phrog_8375 ; phrog_2760	PF03837.17 ;PF03837.17
NC_001416.1	32816	33232	-	NP_040618.1	138	16346.28	-3.5	5.7699	gam-like host nuclease inhibitor	phrog_259 ; phrog_26899	PF06064.14
NC_001416.1	33187	33330	-	NP_040619.1	47	5518.58	3.5	10.2786	Kil protein for bacterial septation inhibition	phrog_2461 ; phrog_2055 ; phrog_3924 ; phrog_20024	PF06301.14
NC_001416.1	33299	33463	-	NP_040620.1	54	6046.92	2.0	9.0019	CIII anti-termination	phrog_2461 ; phrog_35423 ; phrog_550	PF02061.19
NC_001416.1	33536	33904	-	NP_040621.1	122	13780.39	-12.0	4.0718	hypothetical protein	phrog_646	PF10800.11
NC_001416.1	34087	34287	-	NP_040622.1	66	7603.86	3.0	8.5100	restriction alleviation ral	phrog_1055 ; phrog_17610	PF11058.11
NC_001416.1	34271	34357	-	NP_040623.1	28	3698.81	-11.5	3.2418	hypothetical protein

Seed genomic context- JBrowse

PDB structure: 2UV1 ; Download help

Full Sequence Download help

Structural homologs

Structure homologs against by afdb (html) ; download full result table

Hit	Query start	Query end	Hit start	Hit end	TMscore(ali)	TMscore(avg)
AF-Q32GM6-F1-model_v4	1	78	20	97	0.9773	0.8705

No homologs found in esmfold database

Family information Download help

Fam ID	Seed protein	Member count	Model	Alignment
APIS092	NP_040618.1	14	HMM model	Member alignment

Host Taxa distribution

Length distribution

Homology to other families help

Clan ID: CLAN021

APIS092 has no similar APIS family.

Family members, sources, and their hosts help

Results of representative protein blast against members.
Member	pident	evalue	bitscore	qcovs	host	SourceDB	Pfam	Phrog
IMGVR_UViG_2513237251_000004\|2513237251\|2514249572	97.101	1.22e-96	282	100%	Escherichia coli	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_2706796269_000001\|2706796269\|2708712766	97.101	2.74e-96	281	100%	Salmonella	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_2713896771_000008\|2713896771\|2714177310	82.443	5.35e-71	219	93%	Escherichia coli	IMGVR	PF06064	phrog_294 phrog_259 phrog_26899
IMGVR_UViG_2974709687_000001\|2974709687\|2974709722	97.101	5.61e-95	278	100%	Proteobacteria	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_2974711179_000001\|2974711179\|2974711192	96.377	3.81e-96	281	100%	Salmonella	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_3300002231_001651\|3300002231\|KVRMV2_1001172879	97.656	4.73e-89	264	93%	Proteobacteria	IMGVR	PF06301 PF06064	phrog_3924 phrog_259 phrog_26899 phrog_2461 phrog_20024
IMGVR_UViG_3300009612_000002\|3300009612\|Ga0105217_10014234	100.000	6.07e-99	288	100%	Proteobacteria	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_3300029450_000004\|3300029450\|Ga0244073_10026657	95.652	2.29e-94	276	100%	Enterobacteriaceae	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_3300039333_000048\|3300039333\|Ga0169237_03033_2010_2426	98.551	3.71e-98	286	100%	Gammaproteobacteria	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_3300045988_066558\|3300045988\|Ga0495776_161089_24453_24869	97.101	1.75e-96	282	100%	Enterobacteriaceae	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_3300045988_117741\|3300045988\|Ga0495776_100461_28810_29226	97.101	7.53e-96	280	100%	Gammaproteobacteria	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_3300045988_132460\|3300045988\|Ga0495776_144655_27985_28401	97.826	1.34e-97	285	100%	Gammaproteobacteria	IMGVR	PF06064	phrog_259 phrog_26899
IMGVR_UViG_638275567_000001\|638275567\|638277377	96.377	6.89e-96	280	100%	Enterobacteriaceae	IMGVR	PF06064	phrog_259 phrog_26899
NP_040618.1	100.000	4.78e-106	288	100%		NCBI	PF06064	phrog_259 phrog_26899