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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0001

PubMed

30796211, Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.

Characterization method

RNA-Seq,substrate binding assay,enzyme activity assay,mass spectrometry

Genomic accession number

NZ_GG692714.1

Nucelotide position range

156723-175880

Substrate

beta-mannan

Loci

ROSINTL182_05469-ROSINTL182_05483

Species

Roseburia intestinalis/166486

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

manA ROSINTL182_05469 EEV02612.1 0 - 1569 (-) GG692714.1:156723-158292 5.3.1.8
- ROSINTL182_05470 EEV02613.1 1606 - 2305 (-) GG692714.1:158329-159028 -
- ROSINTL182_05471 EEV02614.1 2328 - 3447 (-) GG692714.1:159051-160170 -
- ROSINTL182_05472 EEV02615.1 3485 - 3758 (-) GG692714.1:160208-160481 -
- ROSINTL182_05473 EEV02550.1 3858 - 4637 (-) GG692714.1:160581-161360 -
- ROSINTL182_05474 EEV02551.1 4730 - 5753 (-) GG692714.1:161453-162476 -
- ROSINTL182_05475 EEV02552.1 5835 - 7029 (-) GG692714.1:162558-163752 -
- ROSINTL182_05476 EEV02553.1 7057 - 8287 (-) GG692714.1:163780-165010 5.1.3.8
- ROSINTL182_05477 EEV02554.1 8308 - 9184 (-) GG692714.1:165031-165907 -
- ROSINTL182_05478 EEV02555.1 9188 - 10214 (-) GG692714.1:165911-166937 -
- ROSINTL182_05479 EEV02556.1 10264 - 11734 (-) GG692714.1:166987-168457 -
- ROSINTL182_05480 EEV02557.1 13098 - 14124 (-) GG692714.1:169821-170847 -
- ROSINTL182_05481 EEV02558.1 14166 - 16356 (-) GG692714.1:170889-173079 -
- ROSINTL182_05482 EEV02559.1 16372 - 18091 (-) GG692714.1:173095-174814 -
- ROSINTL182_05483 EEV02560.1 18222 - 19158 (-) GG692714.1:174945-175881 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

manA 1 - 1569 (-) CAZyme: GH1 Yes
- 1607 - 2305 (-) TC: gnl|TC-DB|Q9UEF7|8.A.49.1.1 Yes
- 2329 - 3447 (-) other Yes
- 3486 - 3758 (-) other Yes
- 3859 - 4637 (-) CAZyme: CE2 Yes
- 4731 - 5753 (-) CAZyme: GH130 Yes
- 5836 - 7029 (-) CAZyme: GH130 Yes
- 7058 - 8287 (-) other Yes
- 8309 - 9184 (-) TC: gnl|TC-DB|Q8DT26|3.A.1.1.27 Yes
- 9189 - 10214 (-) TC: gnl|TC-DB|Q9X9R6|3.A.1.1.23 Yes
- 10265 - 11734 (-) STP: STP|SBP_bac_1 Yes
- 13099 - 14124 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 Yes
- 14167 - 16356 (-) CAZyme: GH36 Yes
- 16373 - 18091 (-) other Yes
- 18223 - 19158 (-) CAZyme: GH113 Yes

PUL ID

PUL0001

PubMed

30796211, Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.

Title

The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans.

Author

La Rosa SL, Leth ML, Michalak L, Hansen ME, Pudlo NA, Glowacki R, Pereira G, Workman CT, Arntzen MO, Pope PB, Martens EC, Hachem MA, Westereng B

Abstract

beta-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of beta-mannans. We combine multi-"omic" analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in beta-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available beta-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment. In murine experiments, beta-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using beta-mannan-based therapeutic interventions.