PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
---|---|---|---|---|---|---|---|---|---|
PUL0001 | RNA-Seq, substrate binding assay, enzyme activity assay, mass spectrometry | beta-mannan | Roseburia intestinalis | 30796211 The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y. |
2019 Feb 22 | degradation | 15 | 7 | GH1, CE2, GH130, GH130, GH36, GH113 |
PUL0002 | enzyme activity assay, Northern Blot | lichenan | Bacillus subtilis | 8606172 LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family. J Bacteriol. 1996 Apr;178(7):1971-9. doi: 10.1128/jb.178.7.1971-1979.1996. |
1996 Apr | degradation | 2 | 1 | GH16 |
PUL0003 | RT-PCR | xylan | Bacillus subtilis | 26559526 Metabolic potential of Bacillus subtilis 168 for the direct conversion of xylans to fermentation products. Appl Microbiol Biotechnol. 2016 Feb;100(3):1501-1510. doi: 10.1007/s00253-015-7124-x. Epub 2015 Nov 12. |
2016 Feb | degradation | 2 | 2 | GH30, GH30_8, GH43_16, CBM6 |
PUL0004 | enzyme activity assay, substrate binding assay | glucose, cellobiose, maltose | uncultured bacterium | 26827771 A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase. Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15. |
2016 Mar | degradation | 2 | 1 | GH1 |
PUL0005 | enzyme activity assay, crystallization | beta-glucan, sophorose, laminaribiose | Listeria innocua | 26886583 Functional and Structural Analysis of a beta-Glucosidase Involved in beta-1,2-Glucan Metabolism in Listeria innocua. PLoS One. 2016 Feb 17;11(2):e0148870. doi: 10.1371/journal.pone.0148870. eCollection 2016. |
2016 | degradation | 2 | 1 | GH94, GH3 |
PUL0006 | enzyme activity assay | galactan | Geobacillus stearothermophilus | 24637762 Purification, crystallization and preliminary crystallographic analysis of Gan1D, a GH1 6-phospho-beta-galactosidase from Geobacillus stearothermophilus T1. Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):225-31. doi: 10.1107/S2053230X13034778. Epub 2014 Jan 21. |
2014 Feb | degradation | 11 | 2 | GH1 |
PUL0007 | sequence homology analysis | galactan | Leuconostoc gelidum | 27274361 Complete genome sequence of Leuconostoc gelidum subsp. gasicomitatum KG16-1, isolated from vacuum-packaged vegetable sausages. Stand Genomic Sci. 2016 Jun 7;11:40. doi: 10.1186/s40793-016-0164-8. eCollection 2016. |
2016 | degradation | 8 | 2 | GH53, GH42 |
PUL0008 | enzyme activity assay | fructan | Bacteroides thetaiotaomicron | 28103254 A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. PLoS One. 2017 Jan 19;12(1):e0169989. doi: 10.1371/journal.pone.0169989. eCollection 2017. |
2017 | degradation | 12 | 2 | GH32, GH32, GH32 |
PUL0009 | enzyme activity assay, immunoblotting, ATPase assay | glycosaminoglycan | Streptobacillus moniliformis | 28432302 A bacterial ABC transporter enables import of mammalian host glycosaminoglycans. Sci Rep. 2017 Apr 21;7(1):1069. doi: 10.1038/s41598-017-00917-y. |
2017 Apr 21 | degradation | 15 | 4 | PL8, PL8, GH88 |
PUL0010 | enzyme activity assay, liquid chromatography and mass spectrometry | xylan | Geobacillus thermodenitrificans | 28616644 Synergistic hydrolysis of xylan using novel xylanases, beta-xylosidases, and an alpha-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Appl Microbiol Biotechnol. 2017 Aug;101(15):6023-6037. doi: 10.1007/s00253-017-8341-2. Epub 2017 Jun 14. |
2017 Aug | degradation | 42 | 9 | GH43, GH43_11, GH10, GH39, GH67, GH10, CBM22, GH52, CE4, GH51 |
PUL0011 | enzyme activity assay | cellulose | Ruminiclostridium cellulolyticum | 8936327 Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli. Microbiology (Reading). 1996 Apr;142 ( Pt 4):1013-1023. doi: 10.1099/00221287-142-4-1013. |
1996 Apr | degradation | 4 | 4 | GH48, GH8, CBM3, GH9, CBM4, GH9 |
PUL0012 | enzyme activity assay | chitin | Vibrio cholerae | 28683122 The nucleoid occlusion protein SlmA is a direct transcriptional activator of chitobiose utilization in Vibrio cholerae. PLoS Genet. 2017 Jul 6;13(7):e1006877. doi: 10.1371/journal.pgen.1006877. eCollection 2017 Jul. |
2017 Jul | degradation | 11 | 2 | GH94, GH20, GH9 |
PUL0013 | Northern Blot, RT-PCR, isothermal titration calorimetry, electrophoretic mobility shift assay | arabinan | Geobacillus stearothermophilus | 21460081 The L-Arabinan utilization system of Geobacillus stearothermophilus. J Bacteriol. 2011 Jun;193(11):2838-50. doi: 10.1128/JB.00222-11. Epub 2011 Apr 1. |
2011 Jun | degradation | 56 | 11 | GH43_5, GH43, GH43, GH43_4, GH51, GH51, GH127, CE4, GH52, GH10, GH67, GH39, GH43_11 |
PUL0014 | sequence homology analysis, growth assay | pectin | Geobacillus thermodenitrificans | 28900693 Complete Genome Sequence of Geobacillus thermodenitrificans T12, A Potential Host for Biotechnological Applications. Curr Microbiol. 2018 Jan;75(1):49-56. doi: 10.1007/s00284-017-1349-0. Epub 2017 Sep 12. |
2018 Jan | degradation | 9 | 2 | PL1_6, PL1, GH105 |
PUL0015 | microarray | cellobiose | Lactococcus lactis | 28970222 Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363. Appl Environ Microbiol. 2017 Nov 16;83(23):e01279-17. doi: 10.1128/AEM.01279-17. Print 2017 Dec 1. |
2017 Dec 1 | degradation | 4 | 1 | GH9 |
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