Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0005 enzyme activity assay, crystallization beta-glucan, sophorose, laminaribiose Listeria innocua 26886583
Functional and Structural Analysis of a beta-Glucosidase Involved in beta-1,2-Glucan Metabolism in Listeria innocua. PLoS One. 2016 Feb 17;11(2):e0148870. doi: 10.1371/journal.pone.0148870. eCollection 2016.
2016 degradation 2 1 GH94, GH3
PUL0044 qRT-PCR, enzyme activity assay arabinoxylan Bacteroides ovatus 26112186, 32266006
Glycan complexity dictates microbial resource allocation in the large intestine. Multimodular fused acetyl-feruloyl esterases from soil and gut Bacteroidetes improve xylanase depolymerization of recalcitrant biomass. Nat Commun. 2015 Jun 26;6:7481. doi: 10.1038/ncomms8481. Biotechnol Biofuels. 2020 Mar 31;13:60. doi: 10.1186/s13068-020-01698-9. eCollection 2020.
2015 Jun 26,2020 degradation 33 16 GH43, GH43_10, GH3, GH43_12, GH43, GH31, GH97, CBM6, GH43, GH43_29, GH43_12, GH43, GH30_8, CBM35, GH98, GH115, CE6, CE1, CBM6, GH43, GH43_29, GH95, GH30, GH115
PUL0050 fosmid library screen cellulose, galactomannan feces metagenome 29601586
Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018.
2018 degradation 20 7 GH5_4, GH5, GH26, GH130, GH3, GH94
PUL0063 bicinchoninic acid (BCA) reducing-sugar assay, enzymatic product analysis, affinity gel electrophoresis, isothermal titration calorimetry xyloglucan Bacteroides ovatus 31420336
Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15.
2019 Oct 15 degradation 16 8 GH3, GH2, GH31, GH9, GH5_4, GH43, GH43_12, GH43_12, GH3
PUL0064 bicinchoninic acid (BCA) reducing-sugar assay, enzymatic product analysis, affinity gel electrophoresis, isothermal titration calorimetry xyloglucan Bacteroides cellulosilyticus 31420336
Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15.
2019 Oct 15 degradation 12 4 GH5, GH5_4, GH31, GH2, GH3
PUL0065 bicinchoninic acid (BCA) reducing-sugar assay, enzymatic product analysis, affinity gel electrophoresis, isothermal titration calorimetry xyloglucan Bacteroides uniformis 31420336
Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15.
2019 Oct 15 degradation 13 6 GH43_12, GH43, GH5_4, GH5, GH31, GH2, GH3, GH95
PUL0066 bicinchoninic acid (BCA) reducing-sugar assay, enzymatic product analysis, affinity gel electrophoresis, isothermal titration calorimetry xyloglucan Bacteroides fluxus 31420336
Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15.
2019 Oct 15 degradation 13 6 GH43_12, GH43, GH5, GH5_4, GH31, GH2, GH3, GH95
PUL0067 bicinchoninic acid (BCA) reducing-sugar assay, enzymatic product analysis, affinity gel electrophoresis, isothermal titration calorimetry xyloglucan Dysgonomonas gadei 31420336
Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15.
2019 Oct 15 degradation 11 6 GH2, GH5_4, GH5, GH31, GH95, GH3, GH43_12
PUL0099 RNA-Seq, substrate binding assay, enzyme activity assay, mass spectrometry beta-mannan Roseburia intestinalis 30796211
The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.
2019 Feb 22 degradation 3 2 CBM27, GH26, CBM23, GH3, GH3
PUL0101 sequence homology analysis O-glycan, N-glycan Bacteroides plebeius 31275257
Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.
2019 degradation 19 5 CBM67, GH78, GH3, GH115, GH97
PUL0105 fosmid library screen, lectin binding assay O-glycan, N-glycan uncultured bacterium 31275257
Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.
2019 degradation 36 5 GH33, CBM67, GH78, GH3, GH115, GH97
PUL0108 sequence homology analysis O-glycan, N-glycan Bacteroides uniformis 31275257
Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.
2019 degradation 27 3 GH2, GH3
PUL0118 qRT-PCR, affinity gel electrophoresis, isothermal titration calorimetry laminarin, beta-glucan Bacteroides uniformis 32265336
Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides. mBio. 2020 Apr 7;11(2):e00095-20. doi: 10.1128/mBio.00095-20.
2020 Apr 7 degradation 7 3 CBM6, GH3, GH158, GH16
PUL0125 affinity gel electrophoresis, substrate binding assay, RNA-Seq beta-glucan Bacteroides ovatus 31062073, 32801182
Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Mol Life Sci. 2019 Nov;76(21):4319-4340. doi: 10.1007/s00018-019-03115-3. Epub 2019 May 6. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1.
2019 Nov,2020 Oct 1 degradation 6 2 GH16, GH3
PUL0164 mass spectrometry, sequence homology analysis, differential gene expression beta-mannan Leeuwenhoekiella sp. MAR_2009_132 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 19 9 CE2, GH3, GH5_7, GH26, GH130, GH26, GH5_2, GH5, GH27, GH9, GH26
PUL0170 enzyme activity assay beta-glucan Bacteroides ovatus 29141231
Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Cell Rep. 2017 Nov 14;21(7):2030. doi: 10.1016/j.celrep.2017.11.013.
2017 Nov 14 degradation 7 3 GH3, GH16, GH3
PUL0211 enzyme activity assay, gene deletion mutant and growth assay, thin layer chromatography N-glycan Xanthomonas campestris pv. campestris 25586188, 25205095
The N-Glycan cluster from Xanthomonas campestris pv. campestris: a toolbox for sequential plant N-glycan processing. The plant pathogen Xanthomonas campestris pv. campestris exploits N-acetylglucosamine during infection. J Biol Chem. 2015 Mar 6;290(10):6022-36. doi: 10.1074/jbc.M114.624593. Epub 2015 Jan 13. mBio. 2014 Sep 9;5(5):e01527-14. doi: 10.1128/mBio.01527-14.
2015 Mar 6,2014 Sep 9 degradation 9 6 GH29, GH18, GH20, GH2, GH3, GH125, GH92, GH35
PUL0220 mass spectrometry, target decoy database analysis laminarin Polaribacter sp. Hel1_33_49 25478683
Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. ISME J. 2015 Jun;9(6):1410-22. doi: 10.1038/ismej.2014.225. Epub 2014 Dec 5.
2015 Jun degradation 11 5 GH30, GH30_1, GH17, GH3, GH149, GH16
PUL0221 fosmid library screen cellulose uncultured bacterium Contig1529 24223817
Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013.
2013 degradation 10 5 GH35, GH105, GH3, GH5_4
PUL0227 enzyme activity assay, substrate binding assay xylan Caldanaerobius polysaccharolyticus 22918832
Biochemical and structural insights into xylan utilization by the thermophilic bacterium Caldanaerobius polysaccharolyticus. J Biol Chem. 2012 Oct 12;287(42):34946-34960. doi: 10.1074/jbc.M112.391532. Epub 2012 Aug 22.
2012 Oct 12 degradation 12 1 GH67, GH3
PUL0234 proteome fractionation, mass spectrometry, target decoy database analysis laminarin Gramella forsetii 24522261
Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13.
2014 Jul degradation 7 3 GH16, GH3, GH16
PUL0239 fosmid library screen, sequence homology analysis cellulose Prevotella sp. Sc00026 24448980
Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
2014 Mar degradation 18 10 GH26, GH5_4, GH5_7, GH130, GH26, CE7, GH36, GH3
PUL0269 RT-PCR chitin Haloferax mediterranei 23674154
Characterization of genes for chitin catabolism in Haloferax mediterranei. Appl Microbiol Biotechnol. 2014 Feb;98(3):1185-94. doi: 10.1007/s00253-013-4969-8. Epub 2013 May 15.
2014 Feb degradation 16 4 GH3, CBM5, CBM5, GH18, CBM5, GH18
PUL0289 enzyme activity assay xylan Flavobacterium johnsoniae 29588659
A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans. Biotechnol Biofuels. 2018 Mar 22;11:74. doi: 10.1186/s13068-018-1074-3. eCollection 2018.
2018 degradation 12 7 GH97, GH3, CBM22, GH43_10, GH43, GH146, GH115, CE0, CE6, CE1, GH43_12
PUL0292 enzyme activity assay chitin Collimonas fungivorans 18671744
Identification and characterization of genes underlying chitinolysis in Collimonas fungivorans Ter331. FEMS Microbiol Ecol. 2008 Oct;66(1):123-35. doi: 10.1111/j.1574-6941.2008.00547.x. Epub 2008 Jul 30.
2008 Oct degradation 14 3 GH0, GH16, GH3
PUL0308 microarray, enzyme activity assay, high performance anion exchange chromatography, mass spectrometry, RNA-seq beta-glucan Bacteroides ovatus 29020628, 22205877, 32801182
Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Rep. 2017 Oct 10;21(2):417-430. doi: 10.1016/j.celrep.2017.09.049. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1.
2017 Oct 10,2011 Dec,2020 Oct 1 degradation 5 3 GH16, GH3
PUL0325 RT-PCR, enzyme activity assay beta-glucan Bacteroides thetaiotaomicron 28461332
A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase. J Biol Chem. 2017 Jun 23;292(25):10639-10650. doi: 10.1074/jbc.M117.787606. Epub 2017 May 1.
2017 Jun 23 degradation 6 2 GH30, GH30_3, GH3
PUL0329 microarray, gas chromatography and mass spectrometry xylan Gramella flava 28261179
Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017.
2017 degradation 25 7 GH43_10, GH43, CBM22, GH43, GH43_12, GH43, GH43_1, GH3, GH67, GH10, GH115, CE15
PUL0335 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 31 4 GH13, GH97, GH3, GH158, GH16
PUL0336 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 25 3 GH3, GH158, GH16
PUL0337 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 29 3 GH16, GH158, GH3
PUL0338 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 34 4 GH16, GH158, GH3, GH97
PUL0339 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 24 4 GH97, GH3, GH16, GH20
PUL0342 enzyme activity assay, gene deletion mutant and growth assay xylan Prevotella ruminicola 19304844
Biochemical analysis of a beta-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23. J Bacteriol. 2009 May;191(10):3328-38. doi: 10.1128/JB.01628-08. Epub 2009 Mar 20.
2009 May degradation 5 3 GH10, CE1, CBM22, GH95, GH3
PUL0390 enzyme activity assay acetylated glucuronoxylan Thermotoga maritima 21255309
Hyperthermostable acetyl xylan esterase. Microb Biotechnol. 2010 Jan;3(1):84-92. doi: 10.1111/j.1751-7915.2009.00150.x. Epub 2009 Sep 18.
2010 Jan degradation 24 5 GH67, GH10, CBM22, CBM9, CBM22, GH10, GH3, CE7
PUL0401 RNA-Seq laminarin Alteromonas macleodii 30116038
Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J. 2019 Jan;13(1):92-103. doi: 10.1038/s41396-018-0252-4. Epub 2018 Aug 16.
2019 Jan degradation 9 3 CBM4, GH16, GH3
PUL0406 high performance anion exchange chromatography beta-glucan Coprothermobacter proteolyticus 30315317
From proteins to polysaccharides: lifestyle and genetic evolution of Coprothermobacter proteolyticus. ISME J. 2019 Mar;13(3):603-617. doi: 10.1038/s41396-018-0290-y. Epub 2018 Oct 12.
2019 Mar degradation 20 3 GH16, GH3, GH18
PUL0420 microarray plant polysaccharide Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 4 1 GH144, GH3
PUL0474 growth assay beta-glucan, xylan Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 9 5 GH30_1, GH30, GH30_1, GH30, GH3, GH30_3, GH3
PUL0480 growth assay hemicellulose Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 9 4 GH3, GH10, GH16, GH8
PUL0481 growth assay, enzyme activity assay hemicellulose Flavobacterium johnsoniae 19717629, 32266006
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Multimodular fused acetyl-feruloyl esterases from soil and gut Bacteroidetes improve xylanase depolymerization of recalcitrant biomass. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. Biotechnol Biofuels. 2020 Mar 31;13:60. doi: 10.1186/s13068-020-01698-9. eCollection 2020.
2009 Nov,2020 degradation 13 7 GH97, GH3, GH43, GH43_10, CBM22, GH146, GH115, GH43_12, GH3
PUL0508 clone and expression, enzyme activity assay xylobiose, xylotriose Streptomyces thermoviolaceus 14761997
Molecular characterization of a high-affinity xylobiose transporter of Streptomyces thermoviolaceus OPC-520 and its transcriptional regulation. J Bacteriol. 2004 Feb;186(4):1029-37. doi: 10.1128/JB.186.4.1029-1037.2004.
2004 Feb degradation 5 2 GH3
PUL0526 microarray, qPCR beta-glucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 6 2 GH30_3, GH30, GH3
PUL0527 microarray, qPCR xyloglucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 16 8 GH3, GH2, GH31, GH9, GH5_4, GH43_12, GH43_12, GH3
PUL0528 microarray, qPCR beta-glucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 7 3 GH3, GH16, GH3
PUL0529 microarray, qPCR homogalacturonan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 27 13 GH105, CE8, CE12, CE4, CE8, PL1, CE8, PL1_2, PL1, PL1_2, PL1, PL1_2, CE8, PL1, PL1_2, PL1, CE8, PL1_2, CE8, GH28, GH3, GH43_10, CBM6
PUL0537 RNA-Seq beta-glucan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 7 3 GH3, GH3, GH157
PUL0538 RNA-Seq galactomannan, glucomannan, cellobiose, xyloglucan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 15 7 GH130, GH26, GH26, GH5_2, GH5, GH5_7, CE7, GH3
PUL0545 RNA-Seq arabinan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 10 5 GH43_2, CBM6, GH43, GH3, GH43_17, GH9, GH43_7, CBM13, CE1
PUL0547 RNA-Seq mannose Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 11 4 GH130, GH26, GH2, GH3
PUL0550 RNA-Seq N-acetyl-D-galactosamine Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 7 3 CE8, PL1, PL1_2, CE8, GH3
PUL0551 RNA-Seq cellobiose, beta-glucan, glucomannan, laminarin Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 6 2 GH16, GH3
PUL0553 RT-PCR, qPCR xylan Bacteroides xylanisolvens 27142817
Xylan degradation by the human gut Bacteroides xylanisolvens XB1A(T) involves two distinct gene clusters that are linked at the transcriptional level. BMC Genomics. 2016 May 4;17:326. doi: 10.1186/s12864-016-2680-8.
2016 May 4 degradation 21 13 GH43_10, GH43, CBM6, GH3, GH43, GH43_12, GH31, GH97, CBM66, CBM6, GH43, GH43_29, GH43, GH43_12, CBM4, CBM0, GH10, GH5_21, CE1, CE6, CBM6, GH43_29, GH95, GH115
PUL0588 enzyme activity assay, RT-PCR chitin Streptomyces coelicolor 23278377
Enzymatic and genetic characterization of the DasD protein possessing N-acetyl-beta-d-glucosaminidase activity in Streptomyces coelicolor A3(2). FEMS Microbiol Lett. 2013 Mar;340(1):33-40. doi: 10.1111/1574-6968.12069. Epub 2013 Jan 16.
2013 Mar degradation 4 1 GH3
PUL0597 enzyme activity assay, enzyme kinetic analysis arabinoxylan, arabino-xylooligosaccharide Corynebacterium alkanolyticum 25862223
Functional Characterization of Corynebacterium alkanolyticum beta-Xylosidase and Xyloside ABC Transporter in Corynebacterium glutamicum. Appl Environ Microbiol. 2015 Jun 15;81(12):4173-83. doi: 10.1128/AEM.00792-15. Epub 2015 Apr 10.
2015 Jun 15 degradation 5 1 GH3
PUL0610 enzyme activity assay, strcutural analysis xylan Rhodothermus marinus 31992772
Characterization and diversity of the complete set of GH family 3 enzymes from Rhodothermus marinus DSM 4253. Sci Rep. 2020 Jan 28;10(1):1329. doi: 10.1038/s41598-020-58015-5.
2020 Jan 28 degradation 14 6 GH43_15, CBM6, GH10, CBM4, 3.2.1.8, GH10, GH67, GH3, GH3
PUL0621 RNA-Seq pectin Prevotella sp. PROD 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 8 4 3.2.1.37, GH3, CBM6, GH57, GT4, GH133
PUL0622 RNA-Seq xylan Prevotella sp. PROD 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 15 5 CBM35, CBM2, PL11_1, PL11, GH3, 3.2.1.55, CBM6, 3.2.1.37, GH43_7, CBM13, GH43, PL11_1, CBM35, CE12, PL11, CBM2, GH2
PUL0623 RNA-Seq pectin Prevotella sp. PMUR 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 15 1 CBM6, GH3, 3.2.1.37
PUL0643 enzyme activity assay, liquid chromatography, high performance anion exchange chromatography, qRT-PCR, crystallization arabinoxylan Bacteroides intestinalis DSM 17393 33469030
Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5.
2021 Jan 19 degradation 12 7 CBM48, CE1, GH43, GH43_7, CBM13, CBM48, CE1, CE6, CBM48, CE1, GH43_17, CBM6, GH3, GH43, CBM6, GH43_2
PUL0645 enzyme activity assay, liquid chromatography, high performance anion exchange chromatography, qRT-PCR, crystallization arabinoxylan Bacteroides oleiciplenus YIT 12058 33469030
Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5.
2021 Jan 19 degradation 10 5 GH43, GH43_2, CBM6, GH3, CBM6, GH43_17, GH43_7, GH43, CBM13, CE1, CBM48
PUL0646 recombinant protein expression, crystallization, affinity gel electrophoresis, isothermal titration calorimetry beta-glucan Bacteroides fluxus YIT 12057 33587952
Distinct protein architectures mediate species-specific beta-glucan binding and metabolism in the human gut microbiota. J Biol Chem. 2021 Jan-Jun;296:100415. doi: 10.1016/j.jbc.2021.100415. Epub 2021 Feb 13.
2021 Jan-Jun degradation 6 2 GH3, CBM6, GH158
PUL0654 sequence homology analysis alginate Maribacter dokdonensis 62-1 33912144
CAZymes in Maribacter dokdonensis 62-1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain. Front Microbiol. 2021 Apr 12;12:628055. doi: 10.3389/fmicb.2021.628055. eCollection 2021.
2021 degradation 38 8 GH144, GH144, CE0, CE1, CBM6, GH3, PL6_1, PL6, PL12, PL6_1, PL6, PL17, PL17_2