Browse dbCAN-PUL Entries

PULID	Characterization Method(s)	Substrate	Organism	Publication	Publish Date	Type	Num Genes	Num CAZymes	CazyFamily
PUL0101	sequence homology analysis	O-glycan, N-glycan	Bacteroides plebeius	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	19	5	CBM67, GH78, GH3, GH115, GH97
PUL0102	fosmid library screen, lectin binding assay	O-glycan, N-glycan	uncultured bacterium	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	25	4	GH20, GH29, GH33, CBM67, GH78
PUL0103	fosmid library screen, lectin binding assay	O-glycan, N-glycan	uncultured bacterium	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	31	2	CBM67, GH78, GH33
PUL0104	fosmid library screen, lectin binding assay	O-glycan, N-glycan	uncultured bacterium	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	33	3	GH33, CBM67, GH78
PUL0105	fosmid library screen, lectin binding assay	O-glycan, N-glycan	uncultured bacterium	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	36	5	GH33, CBM67, GH78, GH3, GH115, GH97
PUL0123	fosmid library screen	4-methylumbelliferyl 6-azido-6-deoxy-beta-D-galactoside	uncultured bacterium	31164449 High-Throughput Recovery and Characterization of Metagenome-Derived Glycoside Hydrolase-Containing Clones as a Resource for Biocatalyst Development. mSystems. 2019 Jun 4;4(4):e00082-19. doi: 10.1128/mSystems.00082-19.	2019 Jun 4	degradation	37	2	GH63, CBM67, GH78
PUL0189	RNA-Seq, RT-PCR, qPCR	pectin	Bacteroides xylanisolvens	26920945 Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.	2016 Feb 27	degradation	17	7	GH95, GH140, CBM67, GH78, GH33, GH28, GH78, CBM67, GH43_18, GH43, PL1, CE8, PL1_2, GH92
PUL0419	microarray	plant polysaccharide	Bacteroides ovatus	22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.	2011 Dec	degradation	11	5	GH5, GH5_2, GH5_5, GH2, GH5, GH5_5, GH140, GH130, CBM67, GH78
PUL0472	microarray, qPCR	mucin	Bacteroides thetaiotaomicron	18996345 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.	2008 Nov 13	degradation	28	8	GH38, GH92, CBM32, GH2, GH78, CBM67, CE1, GH92, GH92, GH130, GH43_8
PUL0482	growth assay	pectin	Flavobacterium johnsoniae	19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.	2009 Nov	degradation	27	17	GH78, CBM67, GH142, GH95, GH78, CBM67, GH143, GH43_18, GH43, GH28, GH140, PL1, CE8, PL1_2, PL1, CE8, PL1_2, CE8, GH127, PL1, PL1_2, GH139, GH106, GH2, GH137, GH78
PUL0530	microarray, qPCR	rhamnogalacturonan	Bacteroides thetaiotaomicron	22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.	2011 Dec	degradation	54	21	PL1, CE8, PL1_2, GH2, GH139, GH106, GH2, GH2, GH137, GH2, CBM57, GH138, GH78, GH141, GH127, GH95, GH105, GH140, GH78, GH33, CBM67, GH28, GH78, CBM67, GH143, GH142, GH43_18, GH43, PL1, CE8, PL1_2
PUL0552	RNA-Seq	arabinan	Bacteroides cellulosilyticus	23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.	2013	degradation	7	4	PL1_2, CE8, PL1, GH43_18, GH43, GH142, GH143, GH78, CBM67
PUL0558	gene deletion mutant and growth assay, growth assay, enzyme activity assay	rhamnogalacturonan	Bacteroides thetaiotaomicron	28329766 Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725. Epub 2017 Mar 22.	2017 Apr 6	degradation	50	20	GH2, GH139, GH106, GH2, GH2, GH2, CBM57, GH137, GH138, GH78, GH141, GH127, GH95, GH105, GH140, CBM67, GH33, GH78, GH28, CBM67, GH78, GH142, GH143, GH43_18, GH43, CE8, PL1_2, PL1
PUL0607	enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry	porphyran	Wenyingzhuangia fucanilytica strain CZ1127	32520542 Characterization of a Novel Porphyranase Accommodating Methyl-galactoses at Its Subsites. J Agric Food Chem. 2020 Jul 1;68(26):7032-7039. doi: 10.1021/acs.jafc.0c02404. Epub 2020 Jun 22.	2020 Jul 1	degradation	22	8	GH2, CBM67, CBM51, GH141, PL0, GH105, GH154, GH16_11, GH16, 3.2.1.178, GH117, GH16_11, GH16, GH16_14, GH29
PUL0653	gene deletion mutant and growth assay, complementation study, enzyme activity assay, RNA-Seq, electrophoretic mobility shift assay	agar	Streptomyces coelicolor A3(2)	33889146 LacI-Family Transcriptional Regulator DagR Acts as a Repressor of the Agarolytic Pathway Genes in Streptomyces coelicolor A3(2). Front Microbiol. 2021 Apr 6;12:658657. doi: 10.3389/fmicb.2021.658657. eCollection 2021.	2021	degradation	17	4	GH16_16, 3.2.1.81, CBM67, GH2, GH117, 3.2.1.81, GH50