Browse dbCAN-PUL Entries

PULID	Characterization Method(s)	Substrate	Organism	Publication	Publish Date	Type	Num Genes	Num CAZymes	CazyFamily
PUL0135	enzyme activity assay, substrate binding assay	pectin	Pseudoalteromonas sp.	30341080 Biochemical Reconstruction of a Metabolic Pathway from a Marine Bacterium Reveals Its Mechanism of Pectin Depolymerization. Appl Environ Microbiol. 2018 Dec 13;85(1):e02114-18. doi: 10.1128/AEM.02114-18. Print 2019 Jan 1.	2019 Jan 1	degradation	21	8	CE12, CE4, GH28, GH105, GH105, GH43, GH43_10, CE8, PL1_2
PUL0136	sequence homology analysis	pectin	Pseudoalteromonas haloplanktis	30341080 Biochemical Reconstruction of a Metabolic Pathway from a Marine Bacterium Reveals Its Mechanism of Pectin Depolymerization. Appl Environ Microbiol. 2018 Dec 13;85(1):e02114-18. doi: 10.1128/AEM.02114-18. Print 2019 Jan 1.	2019 Jan 1	degradation	26	6	PL1, PL1_5, CE8, PL1, PL1_2, GH105, GH105, GH28
PUL0189	RNA-Seq, RT-PCR, qPCR	pectin	Bacteroides xylanisolvens	26920945 Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.	2016 Feb 27	degradation	17	7	GH95, GH140, CBM67, GH78, GH33, GH28, GH78, CBM67, GH43_18, GH43, PL1, CE8, PL1_2, GH92
PUL0191	RNA-Seq, RT-PCR, qPCR	pectin	Bacteroides xylanisolvens	26920945 Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.	2016 Feb 27	degradation	11	5	GH105, CE8, CE8, CE12, CE4, PL1_2, PL1_2
PUL0305	RT-qPCR, isothermal titration calorimetry, enzyme activity assay, gene deletion mutant and growth assay	homogalacturonan	Bacteroides thetaiotaomicron	29255254 Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.	2018 Feb	degradation	17	7	GH105, CE8, CE4, CE8, CE12, PL1_2, PL1, CE8, PL1_2, PL1, CE8, PL1_2, GH28
PUL0405	RNA-Seq	pectin	Alteromonas macleodii	30116038 Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J. 2019 Jan;13(1):92-103. doi: 10.1038/s41396-018-0252-4. Epub 2018 Aug 16.	2019 Jan	degradation	4	2	PL1, PL1_2, PL1_5, CE8
PUL0482	growth assay	pectin	Flavobacterium johnsoniae	19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.	2009 Nov	degradation	27	17	GH78, CBM67, GH142, GH95, GH78, CBM67, GH143, GH43_18, GH43, GH28, GH140, PL1, CE8, PL1_2, PL1, CE8, PL1_2, CE8, GH127, PL1, PL1_2, GH139, GH106, GH2, GH137, GH78
PUL0529	microarray, qPCR	homogalacturonan	Bacteroides ovatus	22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.	2011 Dec	degradation	27	13	GH105, CE8, CE12, CE4, CE8, PL1, CE8, PL1_2, PL1, PL1_2, PL1, PL1_2, CE8, PL1, PL1_2, PL1, CE8, PL1_2, CE8, GH28, GH3, GH43_10, CBM6
PUL0530	microarray, qPCR	rhamnogalacturonan	Bacteroides thetaiotaomicron	22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.	2011 Dec	degradation	54	21	PL1, CE8, PL1_2, GH2, GH139, GH106, GH2, GH2, GH137, GH2, CBM57, GH138, GH78, GH141, GH127, GH95, GH105, GH140, GH78, GH33, CBM67, GH28, GH78, CBM67, GH143, GH142, GH43_18, GH43, PL1, CE8, PL1_2
PUL0539	RNA-Seq	fucose, galacturonic acid, rhamnose	Bacteroides cellulosilyticus	23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.	2013	degradation	16	7	PL1_2, CE8, PL1, GH28, GH105, GH28, PL11, GH106, GH105
PUL0550	RNA-Seq	N-acetyl-D-galactosamine	Bacteroides cellulosilyticus	23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.	2013	degradation	7	3	CE8, PL1, PL1_2, CE8, GH3
PUL0552	RNA-Seq	arabinan	Bacteroides cellulosilyticus	23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.	2013	degradation	7	4	PL1_2, CE8, PL1, GH43_18, GH43, GH142, GH143, GH78, CBM67
PUL0558	gene deletion mutant and growth assay, growth assay, enzyme activity assay	rhamnogalacturonan	Bacteroides thetaiotaomicron	28329766 Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725. Epub 2017 Mar 22.	2017 Apr 6	degradation	50	20	GH2, GH139, GH106, GH2, GH2, GH2, CBM57, GH137, GH138, GH78, GH141, GH127, GH95, GH105, GH140, CBM67, GH33, GH78, GH28, CBM67, GH78, GH142, GH143, GH43_18, GH43, CE8, PL1_2, PL1
PUL0614	RNA-Seq	pectin	Prevotella sp. PINT	33113351 Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.	2020 Dec 9	degradation	3	1	CBM6, PL1, CE8, PL1_2
PUL0616	RNA-Seq	pectin	Prevotella sp. PINT	33113351 Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.	2020 Dec 9	degradation	9	1	PL1_2