Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0010 enzyme activity assay, liquid chromatography and mass spectrometry xylan Geobacillus thermodenitrificans 28616644
Synergistic hydrolysis of xylan using novel xylanases, beta-xylosidases, and an alpha-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Appl Microbiol Biotechnol. 2017 Aug;101(15):6023-6037. doi: 10.1007/s00253-017-8341-2. Epub 2017 Jun 14.
2017 Aug degradation 42 9 GH43, GH43_11, GH10, GH39, GH67, GH10, CBM22, GH52, CE4, GH51
PUL0013 Northern Blot, RT-PCR, isothermal titration calorimetry, electrophoretic mobility shift assay arabinan Geobacillus stearothermophilus 21460081
The L-Arabinan utilization system of Geobacillus stearothermophilus. J Bacteriol. 2011 Jun;193(11):2838-50. doi: 10.1128/JB.00222-11. Epub 2011 Apr 1.
2011 Jun degradation 56 11 GH43_5, GH43, GH43, GH43_4, GH51, GH51, GH127, CE4, GH52, GH10, GH67, GH39, GH43_11
PUL0061 NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 31421354
Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8.
2019 Oct 1 biosynthesis 35 4 GT2, GT2, GT4, GT2, GT4, CE4
PUL0070 sugar utilization assay, NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 30664967
Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.
2019 May 1 biosynthesis 36 3 GT52, GT0, GT14, GT2, GT25, GT4, CE4
PUL0071 sugar utilization assay, NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 30664967
Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.
2019 May 1 biosynthesis 38 4 GT0, GT14, GT2, GT25, GT4, CE4
PUL0076 sequence homology analysis capsule polysaccharide, outer core capsule polysaccharide Acinetobacter baumannii 31396168
Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118. Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019.
2019 biosynthesis 7 7 GT4, CE4
PUL0077 sequence homology analysis capsule polysaccharide, outer core capsule polysaccharide Acinetobacter baumannii 31396168
Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118. Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019.
2019 biosynthesis 11 7 GT4, CE4
PUL0079 sequence homology analysis capsule polysaccharide, outer core capsule polysaccharide Acinetobacter baumannii 31396168
Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118. Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019.
2019 biosynthesis 16 6 CE4, GT4, GT2
PUL0080 sequence homology analysis capsule polysaccharide, outer core capsule polysaccharide Acinetobacter baumannii 31396168
Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118. Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019.
2019 biosynthesis 12 6 CE4, GT4, GT2
PUL0135 enzyme activity assay, carbohydrate binding assay pectin Pseudoalteromonas sp. 30341080
Biochemical Reconstruction of a Metabolic Pathway from a Marine Bacterium Reveals Its Mechanism of Pectin Depolymerization. Appl Environ Microbiol. 2018 Dec 13;85(1). pii: AEM.02114-18. doi: 10.1128/AEM.02114-18. Print 2019 Jan 1.
2019 Jan 1 degradation 21 8 CE12, CE4, GH28, GH105, GH105, GH43, GH43_10, CE8, PL1_2
PUL0191 RNA-Seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 11 5 GH105, CE8, CE8, CE12, CE4, PL1_2, PL1_2
PUL0270 comparative genomics exopolysaccharide, lipopolysaccharide Nitrosospira multiformis 18390676
Complete genome sequence of Nitrosospira multiformis, an ammonia-oxidizing bacterium from the soil environment. Appl Environ Microbiol. 2008 Jun;74(11):3559-72. doi: 10.1128/AEM.02722-07. Epub 2008 Apr 4.
2008 Jun biosynthesis 15 3 GT4, CE0, CE4
PUL0305 RT-qPCR, isothermal titration calorimetry, enzyme activity assay, gene deletion mutant and growth assay homogalacturonan Bacteroides thetaiotaomicron 29255254
Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.
2018 Feb degradation 17 7 GH105, CE8, CE4, CE8, CE12, PL1_2, PL1, CE8, PL1_2, PL1, CE8, PL1_2, GH28
PUL0334 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 23 5 GH9, GH31, GH9, GH9, CE4
PUL0381 microarray, gene deletion mutant and growth assay chitin Vibrio cholerae 14983042
The Vibrio cholerae chitin utilization program. Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2524-9. doi: 10.1073/pnas.0308707101.
2004 Feb 24 degradation 6 1 CBM12, CE4, GH4
PUL0428 transposon mutagenesis, growth assay exopolysaccharide Staphylococcus epidermidis 8809760
Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol. 1996 Jun;20(5):1083-91. doi: 10.1111/j.1365-2958.1996.tb02548.x.
1996 Jun biosynthesis 5 1 CE4
PUL0483 growth assay pectin Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 12 5 GH2, CE12, CE4, GH28, GH117, GH106, GH105
PUL0503 sequence homology analysis, microscopy capsule polysaccharide Bacteroides fragilis 20829291
Twenty-eight divergent polysaccharide loci specifying within- and amongst-strain capsule diversity in three strains of Bacteroides fragilis. Microbiology (Reading). 2010 Nov;156(Pt 11):3255-3269. doi: 10.1099/mic.0.042978-0. Epub 2010 Sep 9.
2010 Nov biosynthesis 16 6 GT11, GT4, GT2, GT4, CE4, CE0, GT4, GT2
PUL0529 microarray, qPCR homogalacturonan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 27 13 GH105, CE8, CE12, CE4, CE8, PL1, CE8, PL1_2, PL1, PL1_2, PL1, PL1_2, CE8, PL1, PL1_2, PL1, CE8, PL1_2, CE8, GH28, GH3, GH43_10, CBM6
PUL0542 binding assay xylan Geobacillus stearothermophilus 10368143
The glucuronic acid utilization gene cluster from Bacillus stearothermophilus T-6. J Bacteriol. 1999 Jun;181(12):3695-704. doi: 10.1128/JB.181.12.3695-3704.1999.
1999 Jun degradation 30 6 CE4, GH52, GH10, GH67, GH39, GH43_11
PUL0564 microarray, qPCR rhamnogalacturonan Bacteroides thetaiotaomicron 18996345, 22205877
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2008 Nov 13,2011 Dec degradation 39 23 GH106, GH28, GH28, CE12, GH2, GH43, GH43_18, GH42, GH28, CE4, CE0, GH28, GH2, GH27, CBM35, GH35, PL9, PL9_1, PL26, CE12, GH105, PL11, PL11_1, GH105, CE4, CE0, CE6, GH2, PL9_1
PUL0602 sequence homology analysis xylan Parageobacillus thermoglucosidasius 26442136
Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park. Stand Genomic Sci. 2015 Oct 5;10:73. doi: 10.1186/s40793-015-0031-z. eCollection 2015.
2015 degradation 26 6 CBM22, GH10, GH39, GH67, GH10, GH52, CE4