Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0010 enzyme activity assay, liquid chromatography and mass spectrometry xylan Geobacillus thermodenitrificans 28616644
Synergistic hydrolysis of xylan using novel xylanases, beta-xylosidases, and an alpha-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Appl Microbiol Biotechnol. 2017 Aug;101(15):6023-6037. doi: 10.1007/s00253-017-8341-2. Epub 2017 Jun 14.
2017 Aug degradation 42 9 GH43, GH43_11, GH10, GH39, GH67, GH10, CBM22, GH52, CE4, GH51
PUL0013 Northern Blot, RT-PCR, isothermal titration calorimetry, electrophoretic mobility shift assay arabinan Geobacillus stearothermophilus 21460081
The L-Arabinan utilization system of Geobacillus stearothermophilus. J Bacteriol. 2011 Jun;193(11):2838-50. doi: 10.1128/JB.00222-11. Epub 2011 Apr 1.
2011 Jun degradation 56 11 GH43_5, GH43, GH43, GH43_4, GH51, GH51, GH127, CE4, GH52, GH10, GH67, GH39, GH43_11
PUL0045 qRT-PCR arabinoxylan Bacteroides ovatus 26112186
Glycan complexity dictates microbial resource allocation in the large intestine. Nat Commun. 2015 Jun 26;6:7481. doi: 10.1038/ncomms8481.
2015 Jun 26 degradation 11 5 GH67, GH43_1, GH43, GH10, CBM4, GH10
PUL0161 mass spectrometry, sequence homology analysis alpha-mannan Bacteroides thetaiotaomicron 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 26 6 GH97, GH67, GH76, GH92, GH76, GH125
PUL0227 enzyme activity assay, substrate binding assay xylan Caldanaerobius polysaccharolyticus 22918832
Biochemical and structural insights into xylan utilization by the thermophilic bacterium Caldanaerobius polysaccharolyticus. J Biol Chem. 2012 Oct 12;287(42):34946-34960. doi: 10.1074/jbc.M112.391532. Epub 2012 Aug 22.
2012 Oct 12 degradation 12 1 GH67, GH3
PUL0229 RT-PCR aldouronate Paenibacillus sp. JDR-2 17921311
Structure, function, and regulation of the aldouronate utilization gene cluster from Paenibacillus sp. strain JDR-2. J Bacteriol. 2007 Dec;189(24):8863-70. doi: 10.1128/JB.01141-07. Epub 2007 Oct 5.
2007 Dec degradation 8 3 GH67, CBM22, GH10, GH43_12, GH43
PUL0329 microarray, gas chromatography and mass spectrometry xylan Gramella flava 28261179
Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017.
2017 degradation 25 7 GH43_10, GH43, CBM22, GH43, GH43_12, GH43, GH43_1, GH3, GH67, GH10, GH115, CE15
PUL0345 qRT-PCR, enzyme activity assay xylan Bacteroides intestinalis 27681607
Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan. Sci Rep. 2016 Sep 29;6:34360. doi: 10.1038/srep34360.
2016 Sep 29 degradation 31 13 CBM4, GH10, GH35, GH43_12, GH43, GH10, GH43_1, GH43, GH67, GH8, CE6, GH95, CE0, CE1, GH5, GH5_21, GH10, CBM0, GH115
PUL0375 microarray, qPCR alpha-mannan Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 19 5 GH97, GH67, GH76, GH92, GH76, GH125
PUL0390 enzyme activity assay acetylated glucuronoxylan Thermotoga maritima 21255309
Hyperthermostable acetyl xylan esterase. Microb Biotechnol. 2010 Jan;3(1):84-92. doi: 10.1111/j.1751-7915.2009.00150.x. Epub 2009 Sep 18.
2010 Jan degradation 24 5 GH67, GH10, CBM22, CBM9, CBM22, GH10, GH3, CE7
PUL0392 RT-PCR, qPCR xylan Bacteroides xylanisolvens 27142817
Xylan degradation by the human gut Bacteroides xylanisolvens XB1A(T) involves two distinct gene clusters that are linked at the transcriptional level. BMC Genomics. 2016 May 4;17:326. doi: 10.1186/s12864-016-2680-8.
2016 May 4 degradation 8 3 GH67, GH13_14
PUL0456 microarray, RNA-Seq xylan Prevotella bryantii 20622018
Transcriptomic analyses of xylan degradation by Prevotella bryantii and insights into energy acquisition by xylanolytic bacteroidetes. J Biol Chem. 2010 Sep 24;285(39):30261-73. doi: 10.1074/jbc.M110.141788. Epub 2010 Jul 9.
2010 Sep 24 degradation 13 6 CE6, GH43_10, GH43, GH43_1, GH43, GH10, GH67
PUL0542 substrate binding assay xylan Geobacillus stearothermophilus 10368143
The glucuronic acid utilization gene cluster from Bacillus stearothermophilus T-6. J Bacteriol. 1999 Jun;181(12):3695-704. doi: 10.1128/JB.181.12.3695-3704.1999.
1999 Jun degradation 30 6 CE4, GH52, GH10, GH67, GH39, GH43_11
PUL0557 gene deletion mutant and growth assay alpha-mannan Bacteroides thetaiotaomicron 25567280
Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism. Nature. 2015 Jan 8;517(7533):165-169. doi: 10.1038/nature13995.
2015 Jan 8 degradation 13 6 GH97, GH67, GH76, GH92, GH76, GH125
PUL0592 qRT-PCR methylglucuronoarabinoxylan Paenibacillus sp. JDR-2 25063665
GH51 arabinofuranosidase and its role in the methylglucuronoarabinoxylan utilization system in Paenibacillus sp. strain JDR-2. Appl Environ Microbiol. 2014 Oct;80(19):6114-25. doi: 10.1128/AEM.01684-14. Epub 2014 Jul 25.
2014 Oct degradation 8 3 GH67, GH10, GH43_12
PUL0602 sequence homology analysis xylan Parageobacillus thermoglucosidasius 26442136
Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park. Stand Genomic Sci. 2015 Oct 5;10:73. doi: 10.1186/s40793-015-0031-z. eCollection 2015.
2015 degradation 26 6 CBM22, GH10, GH39, GH67, GH10, GH52, CE4
PUL0610 enzyme activity assay, strcutural analysis xylan Rhodothermus marinus 31992772
Characterization and diversity of the complete set of GH family 3 enzymes from Rhodothermus marinus DSM 4253. Sci Rep. 2020 Jan 28;10(1):1329. doi: 10.1038/s41598-020-58015-5.
2020 Jan 28 degradation 14 6 GH43_15, CBM6, GH10, CBM4,, GH10, GH67, GH3, GH3
PUL0617 RNA-Seq xylan Prevotella sp. PINT 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 14 6 GH67, GH10, GH43, GH43_1, GH43_35, GH43, GH5_21, GH5, GH10, CBM0, CBM4,
PUL0648 high performance anion exchange chromatography, substrate binding assay, thin layer chromatography, NMR, mass spectrometry, crystallization glucuronoarabinoxylan Dysgonomonas mossii DSM 22836 33667545
A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2.
2021 Jan-Jun degradation 37 20 CBM0,, CBM4, GH10, GH43_12, GH43, GH43, GH43_1, GH10, GH146, CBM48, CE1, CBM6, CBM36, GH8,, CE6, CBM2, GH43_29, GH43, CBM22, CBM42, CBM66, CBM6, CBM6, GH43_29, GH43, GH97, GH31, GH51, CBM2, GH43_29, GH43, CBM22, CBM42, CBM6, CBM48, CE1, GH10, GH43_10, GH43, GH115, GH10, GH43, GH43_1, GH67