Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0617

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Characterization method

RNA-Seq

Genomic accession number

JABKKG010000005.1

Nucelotide position range

106573-131671

Substrate

xylan

Loci

HPS57_08475-HPS57_08540

Species

Prevotella sp. PINT/2736291

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HPS57_08475 NPD82008.1 0 - 1929 (-) JABKKG010000005.1:106573-108502 -
- HPS57_08480 NPD82009.1 2115 - 3312 (+) JABKKG010000005.1:108688-109885 -
- HPS57_08485 NPD82010.1 3402 - 4809 (+) JABKKG010000005.1:109975-111382 -
- HPS57_08490 NPD82011.1 4805 - 5933 (+) JABKKG010000005.1:111378-112506 -
- HPS57_08495 NPD82012.1 6046 - 7018 (+) JABKKG010000005.1:112619-113591 -
- HPS57_08500 NPD82013.1 7314 - 9384 (-) JABKKG010000005.1:113887-115957 -
- HPS57_08505 NPD82014.1 9578 - 10517 (-) JABKKG010000005.1:116151-117090 -
- HPS57_08510 NPD82015.1 10545 - 11811 (-) JABKKG010000005.1:117118-118384 -
- HPS57_08515 NPD82016.1 11945 - 14096 (-) JABKKG010000005.1:118518-120669 -
- HPS57_08520 NPD82017.1 14112 - 15123 (-) JABKKG010000005.1:120685-121696 -
- HPS57_08525 NPD82018.1 15189 - 16992 (-) JABKKG010000005.1:121762-123565 -
- HPS57_08530 NPD82019.1 17033 - 19886 (-) JABKKG010000005.1:123606-126459 -
- HPS57_08535 NPD82020.1 20015 - 21965 (-) JABKKG010000005.1:126588-128538 -
- HPS57_08540 NPD82021.1 21997 - 25099 (-) JABKKG010000005.1:128570-131672 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1929 (-) CAZyme: GH67|GH67 Yes
- 2116 - 3312 (+) other Yes
- 3403 - 4809 (+) TC: gnl|TC-DB|P94488|2.A.2.3.2 Yes
- 4806 - 5933 (+) CAZyme: GH10| GH10 Yes
- 6047 - 7018 (+) CAZyme: GH43_1|GH43_1 Yes
- 7315 - 9384 (-) CAZyme: GH43_35| GH43_35 Yes
- 9579 - 10517 (-) other Yes
- 10546 - 11811 (-) CAZyme: GH5_21| GH5_21 Yes
- 11946 - 14096 (-) CAZyme: GH10| GH10 Yes
- 14113 - 15123 (-) other Yes
- 15190 - 16992 (-) other Yes
- 17034 - 19886 (-) other Yes
- 20016 - 21965 (-) other Yes
- 21998 - 25099 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes

PUL ID

PUL0617

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Title

Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Author

Galvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E, Strowig T

Abstract

Prevotella spp. are a dominant bacterial genus within the human gut. Multiple Prevotella spp. co-exist in some individuals, particularly those consuming plant-based diets. Additionally, Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates. To investigate the relationship between Prevotella competition and diet, we isolated Prevotella species from the mouse gut, analyzed their genomes and transcriptomes in vivo, and performed competition experiments between species in mice. Diverse dominant Prevotella species compete for similar metabolic niches in vivo, which is linked to the upregulation of specific polysaccharide utilization loci (PULs). Complex plant-derived polysaccharides are required for Prevotella spp. expansion, with arabinoxylans having a prominent impact on species abundance. The most dominant Prevotella species encodes a specific tandem-repeat trsusC/D PUL that enables arabinoxylan utilization and is conserved in human Prevotella copri strains, particularly among those consuming a vegan diet. These findings suggest that efficient (arabino)xylan-utilization is a factor contributing to Prevotella dominance.