Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0058 enzyme activity assay glycogen Bacillus subtilis 8145641
Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation. Mol Microbiol. 1994 Jan;11(1):203-18. doi: 10.1111/j.1365-2958.1994.tb00301.x.
1994 Jan biosynthesis 5 3 GH13, CBM48, GH13_9, GT5, GT35
PUL0267 RT-qPCR glycogen Lactobacillus acidophilus 23879596
A functional glycogen biosynthesis pathway in Lactobacillus acidophilus: expression and analysis of the glg operon. Mol Microbiol. 2013 Sep;89(6):1187-200. doi: 10.1111/mmi.12338. Epub 2013 Aug 16.
2013 Sep biosynthesis 10 4 GH13_9, CBM48, GH13, GT5, GT35, GH13_39, CBM34, GH13
PUL0347 gene deletion mutant and growth assay, RT-PCR sorbitol Bifidobacterium breve 24581150
Comparative genomics of the Bifidobacterium breve taxon. BMC Genomics. 2014 Mar 1;15(1):170. doi: 10.1186/1471-2164-15-170.
2014 Mar 1 degradation 13 1 CBM48, GH13_11
PUL0605 RT-PCR, gene deletion mutant and growth assay glycogen Escherichia coli 33101261, 21029047
Glycogen Metabolism Impairment via Single Gene Mutation in the glgBXCAP Operon Alters the Survival Rate of Escherichia coli Under Various Environmental Stresses. Escherichia coli glycogen genes are organized in a single glgBXCAP transcriptional unit possessing an alternative suboperonic promoter within glgC that directs glgAP expression. Front Microbiol. 2020 Sep 25;11:588099. doi: 10.3389/fmicb.2020.588099. eCollection 2020. Biochem J. 2011 Jan 1;433(1):107-17. doi: 10.1042/BJ20101186.
2020,2011 Jan 1 biosynthesis 5 4 GT35, GT5, GH13, GH13_11, CBM48, GH77, GH13, GH13_9, CBM48
PUL0625 RNA-Seq xylan Prevotella sp. PMUR 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 18 9 GH43_12, GH43, GH115, GH43, GH43_10, GH97, CBM6, GH43_29, GH43, CBM22, CE1, CBM48, CBM51, CBM32, GH95, CBM13, GH115, GH30_8
PUL0643 enzyme activity assay, liquid chromatography, high performance anion exchange chromatography, qRT-PCR, crystallization arabinoxylan Bacteroides intestinalis DSM 17393 33469030
Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5.
2021 Jan 19 degradation 12 7 CBM48, CE1, GH43, GH43_7, CBM13, CBM48, CE1, CE6, CBM48, CE1, GH43_17, CBM6, GH3, GH43, CBM6, GH43_2
PUL0644 enzyme activity assay, liquid chromatography, high performance anion exchange chromatography, qRT-PCR, crystallization arabinoxylan Bacteroides cellulosilyticus DSM 14838 33469030
Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5.
2021 Jan 19 degradation 10 6 GH43, GH43_2, CBM6, GH43_17, GH10, CBM48, CE1, CE1, GH43, CBM13, GH43_7, CBM48, CE1
PUL0645 enzyme activity assay, liquid chromatography, high performance anion exchange chromatography, qRT-PCR, crystallization arabinoxylan Bacteroides oleiciplenus YIT 12058 33469030
Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5.
2021 Jan 19 degradation 10 5 GH43, GH43_2, CBM6, GH3, CBM6, GH43_17, GH43_7, GH43, CBM13, CE1, CBM48
PUL0647 qPCR sucrose Streptococcus mutans UA159 33603728
The Route of Sucrose Utilization by Streptococcus mutans Affects Intracellular Polysaccharide Metabolism. Front Microbiol. 2021 Feb 2;12:636684. doi: 10.3389/fmicb.2021.636684. eCollection 2021.
2021 biosynthesis 5 3 GT35, GT5, CBM48, GH13_9, GH13
PUL0648 high performance anion exchange chromatography, substrate binding assay, thin layer chromatography, NMR, mass spectrometry, crystallization glucuronoarabinoxylan Dysgonomonas mossii DSM 22836 33667545
A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2.
2021 Jan-Jun degradation 37 20 CBM0, 3.2.1.8, CBM4, GH10, GH43_12, GH43, GH43, GH43_1, GH10, GH146, CBM48, CE1, CBM6, CBM36, GH8, 3.2.1.156, CE6, CBM2, GH43_29, GH43, CBM22, CBM42, CBM66, CBM6, CBM6, GH43_29, GH43, GH97, GH31, GH51, CBM2, GH43_29, GH43, CBM22, CBM42, CBM6, CBM48, CE1, GH10, GH43_10, GH43, GH115, GH10, GH43, GH43_1, GH67