dbCAN-PUL

PUL ID	PUL0058
PubMed	8145641, Mol Microbiol. 1994 Jan;11(1):203-18. doi: 10.1111/j.1365-2958.1994.tb00301.x.
Characterization method	enzyme activity assay
Genomic accession number	Z25795.1
Nucelotide position range	463-8375
Substrate	glycogen
Loci	glgBCDAP
Species	Bacillus subtilis/1423
Degradation or Biosynthesis	biosynthesis

PUL ID	PUL0058
PubMed	8145641, Mol Microbiol. 1994 Jan;11(1):203-18. doi: 10.1111/j.1365-2958.1994.tb00301.x.
Title	Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation.
Author	Kiel JA, Boels JM, Beldman G, Venema G
Abstract	Although it has never been reported that Bacillus subtilis is capable of accumulating glycogen, we have isolated a region from the chromosome of B. subtilis containing a glycogen operon. The operon is located directly downstream from trnB, which maps at 275 degrees on the B. subtilis chromosome. It encodes five polypeptides with extensive similarity to enzymes involved in glycogen and starch metabolism in both prokaryotes and eukaryotes. The operon is presumably expressed by an E sigma E-controlled promoter, which was previously identified downstream from trnB. We have observed glycogen biosynthesis in B. subtilis exclusively on media containing carbon sources that allow efficient sporulation. Sporulation-independent synthesis of glycogen occurred after integration of an E sigma A controlled promoter upstream of the operon.