dbCAN-PUL

PUL ID	PUL0643
PubMed	33469030, Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5.
Characterization method	enzyme activity assay,liquid chromatography,high performance anion exchange chromatography,qRT-PCR,crystallization
Genomic accession number	NZ_ABJL02000007.1
Nucelotide position range	167129-189673
Substrate	arabinoxylan
Loci	BACINT_RS04710-BACINT_RS04765
Species	Bacteroides intestinalis DSM 17393/329854
Degradation or Biosynthesis	degradation

Cluster number	1
Gene name	Gene position	Gene type	Found by CGCFinder?
-	1 - 1158 (+)	CAZyme: CE1\|CE0\|CBM48	Yes
-	1189 - 1887 (+)	other	Yes
-	1890 - 3341 (+)	CAZyme: GH43_7\| GH43_7\|CBM13	Yes
-	3774 - 6899 (+)	TC: gnl\|TC-DB\|Q45780\|1.B.14.6.1	Yes
-	6921 - 8642 (+)	other	Yes
-	9084 - 11000 (+)	CAZyme: CE6\|CE0\| CE6\|CE1\|CBM48	Yes
-	11011 - 12159 (+)	CAZyme: CE1\|CE0\|CBM48	Yes
-	12188 - 13168 (+)	other	Yes
-	13304 - 14362 (+)	CAZyme: GH43_17\|GH43_17	Yes
-	14411 - 16999 (+)	CAZyme: GH3\| GH3	Yes
-	17100 - 18431 (+)	CAZyme: CBM6\|GH43_2\| GH43_2\| CBM6	Yes
-	18451 - 22545 (+)	TF: DBD-Pfam\|HTH_AraC,DBD-Pfam\|HTH_AraC,DBD-SUPERFAMILY\|0035607,DBD-SUPERFAMILY\|0035607	No

PUL ID	PUL0643
PubMed	33469030, Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5.
Title	Degradation of complex arabinoxylans by human colonic Bacteroidetes.
Author	Pereira GV, Abdel-Hamid AM, Dutta S, D'Alessandro-Gabazza CN, Wefers D, Farris JA, Bajaj S, Wawrzak Z, Atomi H, Mackie RI, Gabazza EC, Shukla D, Koropatkin NM, Cann I
Abstract	Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.