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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0267

PubMed

23879596, Mol Microbiol. 2013 Sep;89(6):1187-200. doi: 10.1111/mmi.12338. Epub 2013 Aug 16.

Characterization method

RT-qPCR

Genomic accession number

CP000033.3

Nucelotide position range

665968-680930

Substrate

glycogen

Loci

LBA0679-LBA0688

Species

Lactobacillus acidophilus/1579

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

trxB LBA0679 AAV42555.1 13 - 940 (+) CP000033.3:665981-666908 1.8.1.9
glgB LBA0680 AAV42556.1 1117 - 3034 (+) CP000033.3:667085-669002 2.4.1.18
glgC LBA0681 AAV42557.1 3049 - 4195 (+) CP000033.3:669017-670163 2.7.7.27
glgD LBA0682 AAV42558.1 4184 - 5327 (+) CP000033.3:670152-671295 2.7.7.27
- LBA0683 AAV42559.1 5336 - 6767 (+) CP000033.3:671304-672735 2.4.1.21
- LBA0685 AAV42560.1 6785 - 9197 (+) CP000033.3:672753-675165 2.4.1.1
- LBA0686 AAV42561.1 9196 - 10966 (+) CP000033.3:675164-676934 3.2.1.41
- LBA0687 AAV42562.1 11084 - 12809 (+) CP000033.3:677052-678777 5.4.2.2
uvrB LBA0688 AAV42563.1 12911 - 14963 (+) CP000033.3:678879-680931 -
uvrA LBA0689 AAV42564.1 14949 - 14963 (+) CP000033.3:680917-680931 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

trxB 14 - 940 (+) STP: STP|Pyr_redox_2 No
glgB 1118 - 3034 (+) CAZyme: GH13_9|CBM48|GH13 Yes
glgC 3050 - 4195 (+) other Yes
glgD 4185 - 5327 (+) other Yes
- 5337 - 6767 (+) CAZyme: GT5 Yes
- 6786 - 9197 (+) CAZyme: GT35 Yes
- 9197 - 10966 (+) CAZyme: GH13_39|CBM34|GH13 Yes
- 11085 - 12809 (+) other Yes
uvrB 12912 - 14963 (+) other Yes
uvrA 14950 - 14963 (+) TC: gnl|TC-DB|A9WBR9|3.A.1.32.3 Yes

PUL ID

PUL0267

PubMed

23879596, Mol Microbiol. 2013 Sep;89(6):1187-200. doi: 10.1111/mmi.12338. Epub 2013 Aug 16.

Title

A functional glycogen biosynthesis pathway in Lactobacillus acidophilus: expression and analysis of the glg operon.

Author

Goh YJ, Klaenhammer TR

Abstract

Glycogen metabolism contributes to energy storage and various physiological functions in some prokaryotes, including colonization persistence. A role for glycogen metabolism is proposed on the survival and fitness of Lactobacillus acidophilus, a probiotic microbe, in the human gastrointestinal environment. L. acidophilus NCFM possesses a glycogen metabolism (glg) operon consisting of glgBCDAP-amy-pgm genes. Expression of the glg operon and glycogen accumulation were carbon source- and growth phase-dependent, and were repressed by glucose. The highest intracellular glycogen content was observed in early log-phase cells grown on trehalose, which was followed by a drastic decrease of glycogen content prior to entering stationary phase. In raffinose-grown cells, however, glycogen accumulation gradually declined following early log phase and was maintained at stable levels throughout stationary phase. Raffinose also induced an overall higher temporal glg expression throughout growth compared with trehalose. Isogenic DeltaglgA (glycogen synthase) and DeltaglgB (glycogen-branching enzyme) mutants are glycogen-deficient and exhibited growth defects on raffinose. The latter observation suggests a reciprocal relationship between glycogen synthesis and raffinose metabolism. Deletion of glgB or glgP (glycogen phosphorylase) resulted in defective growth and increased bile sensitivity. The data indicate that glycogen metabolism is involved in growth maintenance, bile tolerance and complex carbohydrate utilization in L. acidophilus.