Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0010 enzyme activity assay, liquid chromatography and mass spectrometry xylan Geobacillus thermodenitrificans 28616644
Synergistic hydrolysis of xylan using novel xylanases, beta-xylosidases, and an alpha-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Appl Microbiol Biotechnol. 2017 Aug;101(15):6023-6037. doi: 10.1007/s00253-017-8341-2. Epub 2017 Jun 14.
2017 Aug degradation 42 9 GH43, GH43_11, GH10, GH39, GH67, GH10, CBM22, GH52, CE4, GH51
PUL0013 Northern Blot, RT-PCR, isothermal titration calorimetry, electrophoretic mobility shift assay arabinan Geobacillus stearothermophilus 21460081
The L-Arabinan utilization system of Geobacillus stearothermophilus. J Bacteriol. 2011 Jun;193(11):2838-50. doi: 10.1128/JB.00222-11. Epub 2011 Apr 1.
2011 Jun degradation 56 11 GH43_5, GH43, GH43, GH43_4, GH51, GH51, GH127, CE4, GH52, GH10, GH67, GH39, GH43_11
PUL0139 sequence homology analysis arabinose Bifidobacterium animalis subsp. animalis 30306201
Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions. Appl Microbiol Biotechnol. 2018 Dec;102(24):10645-10663. doi: 10.1007/s00253-018-9413-7. Epub 2018 Oct 10.
2018 Dec degradation 12 1 GH51
PUL0190 RNA-Seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 15 5 GH51, GH146, GH43, GH43_4, GH43_4, GH51, GH43_29
PUL0218 enzyme activity assay arabinose, arabinan termite gut metagenome 25304507
Investigating the function of an arabinan utilization locus isolated from a termite gut community. Appl Environ Microbiol. 2015 Jan;81(1):31-9. doi: 10.1128/AEM.02257-14. Epub 2014 Oct 10.
2015 Jan degradation 24 5 GH51, GH97, GH146, GH43_4, GH43, GH43_4, GH51, GH43_29
PUL0228 qRT-PCR, enzyme activity assay, high performance anion exchange chromatography arabinan Bacteroides thetaiotaomicron 22686399, 21339299
Prioritization of a plant polysaccharide over a mucus carbohydrate is enforced by a Bacteroides hybrid two-component system. The structure and function of an arabinan-specific alpha-1,2-arabinofuranosidase identified from screening the activities of bacterial GH43 glycoside hydrolases. Mol Microbiol. 2012 Aug;85(3):478-91. doi: 10.1111/j.1365-2958.2012.08123.x. Epub 2012 Jul 5. J Biol Chem. 2011 Apr 29;286(17):15483-95. doi: 10.1074/jbc.M110.215962. Epub 2011 Feb 21.
2012 Aug,2011 Apr 29 degradation 10 4 GH43, GH43_4, GH43_4, GH51, GH43_29
PUL0300 gene trait matching exercise arabinoxylan Bifidobacterium longum 29310579
Gene-trait matching across the Bifidobacterium longum pan-genome reveals considerable diversity in carbohydrate catabolism among human infant strains. BMC Genomics. 2018 Jan 8;19(1):33. doi: 10.1186/s12864-017-4388-9.
2018 Jan 8 degradation 14 5 GH43_26, GH43, GH43_27, GH43, GH43_4, GH43_4, GH51
PUL0302 RT-qPCR, isothermal titration calorimetry, enzyme activity assay, gene deletion mutant and growth assay arabinan Bacteroides thetaiotaomicron 29255254
Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.
2018 Feb degradation 22 6 GH51, GH146, GH43_4, GH43, GH43_4, GH51, GH43_29
PUL0309 enzyme activity assay, substrate binding assay, isothermal titration calorimetry arabinan Caldanaerobius polysaccharolyticus 28710263
Enzymatic Mechanism for Arabinan Degradation and Transport in the Thermophilic Bacterium Caldanaerobius polysaccharolyticus. Appl Environ Microbiol. 2017 Aug 31;83(18):e00794-17. doi: 10.1128/AEM.00794-17. Print 2017 Sep 15.
2017 Sep 15 degradation 12 6 GH43_4, GH43, GH127, GH51, GH27, GH51, GH146
PUL0358 microarray lactose, host glycan Bacteroides thetaiotaomicron 16968696
Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12.
2006 Nov 24 degradation 4 4 CBM32, GH35, GH43_19, GH43, GH43_9, GH43_19, GH43, GH51
PUL0395 isothermal calorimetric titration, electrophoretic mobility shift assay, Northern Blot arabinose, arabinan Geobacillus stearothermophilus 21460081
The L-Arabinan utilization system of Geobacillus stearothermophilus. J Bacteriol. 2011 Jun;193(11):2838-50. doi: 10.1128/JB.00222-11. Epub 2011 Apr 1.
2011 Jun degradation 25 5 GH43, GH43_5, GH43, GH43_4, CBM54, GH51, GH51, GH127
PUL0437 mass spectrometry, high performance anion exchange chromatography glucomannan Chitinophaga pinensis 28069559
Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6.
2017 Mar 6 degradation 6 1 GH51
PUL0466 clone and expression, enzyme activity assay, Northern Blot arabinan Bacillus subtilis 14973026
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis. J Bacteriol. 2004 Mar;186(5):1287-96. doi: 10.1128/JB.186.5.1287-1296.2004.
2004 Mar degradation 9 1 GH51
PUL0479 growth assay pectin Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 20 12 GH43, GH43_34, GH43_19, GH27, GH2, GH95, GH51, GH28, GH28, GH2, GH92, GH92, GH89
PUL0532 RNA-Seq arabinan, pectin Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 17 5 GH51, GH43_4, GH43, GH43_4, GH43, GH146, GH51
PUL0546 RNA-Seq arabinogalactan, cellobiose Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 8 4 GH2, GH30_4, GH51
PUL0594 qRT-PCR methylglucuronoarabinoxylan Paenibacillus sp. JDR-2 25063665
GH51 arabinofuranosidase and its role in the methylglucuronoarabinoxylan utilization system in Paenibacillus sp. strain JDR-2. Appl Environ Microbiol. 2014 Oct;80(19):6114-25. doi: 10.1128/AEM.01684-14. Epub 2014 Jul 25.
2014 Oct degradation 4 1 GH51
PUL0620 RNA-Seq xylan Prevotella sp. PROD 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 10 2 GH128, GH51, GH43_19
PUL0624 RNA-Seq xylan Prevotella sp. PMUR 33113351
Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.
2020 Dec 9 degradation 11 3 CBM13, GH43, GH43_24, GH43_19, GH51, GH128
PUL0626 high performance anion exchange chromatography arabinan Lactobacillus crispatus DSM29598 33119797
Characterization of two extracellular arabinanases in Lactobacillus crispatus. Appl Microbiol Biotechnol. 2020 Dec;104(23):10091-10103. doi: 10.1007/s00253-020-10979-0. Epub 2020 Oct 29.
2020 Dec degradation 24 8 GH43, CBM54, GH43_4, GH2, GH43, GH43_4, GH127, GH51, GH43, CBM42, GH43_26, GH51, GH27, CBM51
PUL0648 high performance anion exchange chromatography, substrate binding assay, thin layer chromatography, NMR, mass spectrometry, crystallization glucuronoarabinoxylan Dysgonomonas mossii DSM 22836 33667545
A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2.
2021 Jan-Jun degradation 37 20 CBM0, 3.2.1.8, CBM4, GH10, GH43_12, GH43, GH43, GH43_1, GH10, GH146, CBM48, CE1, CBM6, CBM36, GH8, 3.2.1.156, CE6, CBM2, GH43_29, GH43, CBM22, CBM42, CBM66, CBM6, CBM6, GH43_29, GH43, GH97, GH31, GH51, CBM2, GH43_29, GH43, CBM22, CBM42, CBM6, CBM48, CE1, GH10, GH43_10, GH43, GH115, GH10, GH43, GH43_1, GH67
PUL0666 UHPLC-MS, RNA-seq, RT-qPCR pectin Bacteroides thetaiotaomicron VPI-5482 34420703
Discrete genetic loci in human gut Bacteroides thetaiotaomicron confer pectin metabolism. Carbohydr Polym. 2021 Nov 15;272:118534. doi: 10.1016/j.carbpol.2021.118534. Epub 2021 Aug 6.
2021 Nov 15 degradation 5 4 GH35, GH43, GH51