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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0626

PubMed

33119797, Appl Microbiol Biotechnol. 2020 Dec;104(23):10091-10103. doi: 10.1007/s00253-020-10979-0. Epub 2020 Oct 29.

Characterization method

high performance anion exchange chromatography

Genomic accession number

JAATOH010000049.1

Nucelotide position range

5342-40541

Substrate

arabinan

Loci

HCN73_09280-HCN73_09400

Species

Lactobacillus crispatus DSM29598/47770 

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HCN73_09275 MBA2916503.1 0 - 8 (-) JAATOH010000049.1:5342-5350 -
- HCN73_09280 MBA2916504.1 0 - 1320 (-) JAATOH010000049.1:5342-6662 -
- HCN73_09285 - 1612 - 2966 (-) JAATOH010000049.1:6954-8308 -
- HCN73_09290 - 3134 - 3806 (+) JAATOH010000049.1:8476-9148 -
- HCN73_09295 MBA2916505.1 4386 - 6855 (-) JAATOH010000049.1:9728-12197 -
- HCN73_09300 MBA2916506.1 7141 - 8740 (+) JAATOH010000049.1:12483-14082 -
- HCN73_09305 MBA2916507.1 8758 - 9487 (+) JAATOH010000049.1:14100-14829 -
- HCN73_09310 MBA2916508.1 9627 - 10722 (+) JAATOH010000049.1:14969-16064 -
- HCN73_09315 MBA2916509.1 10860 - 11820 (-) JAATOH010000049.1:16202-17162 -
- HCN73_09320 MBA2916510.1 11803 - 13675 (-) JAATOH010000049.1:17145-19017 -
araA HCN73_09325 MBA2916511.1 13846 - 15274 (+) JAATOH010000049.1:19188-20616 5.3.1.4
- HCN73_09330 MBA2916512.1 15455 - 17120 (-) JAATOH010000049.1:20797-22462 -
- HCN73_09335 MBA2916513.1 17296 - 19243 (+) JAATOH010000049.1:22638-24585 -
- HCN73_09340 MBA2916514.1 19245 - 20694 (+) JAATOH010000049.1:24587-26036 -
- HCN73_09345 MBA2916515.1 20786 - 21416 (-) JAATOH010000049.1:26128-26758 -
- HCN73_09350 MBA2916516.1 21660 - 23019 (+) JAATOH010000049.1:27002-28361 -
- HCN73_09355 MBA2916517.1 23092 - 24010 (+) JAATOH010000049.1:28434-29352 -
- HCN73_09360 MBA2916518.1 24026 - 24875 (+) JAATOH010000049.1:29368-30217 -
- HCN73_09365 MBA2916519.1 24882 - 25554 (+) JAATOH010000049.1:30224-30896 -
- HCN73_09370 MBA2916520.1 25633 - 26578 (+) JAATOH010000049.1:30975-31920 -
- HCN73_09375 MBA2916521.1 26597 - 28091 (+) JAATOH010000049.1:31939-33433 -
- HCN73_09380 MBA2916522.1 28101 - 29124 (+) JAATOH010000049.1:33443-34466 -
- HCN73_09385 MBA2916523.1 29189 - 30458 (+) JAATOH010000049.1:34531-35800 -
- HCN73_09390 MBA2916524.1 30516 - 31626 (-) JAATOH010000049.1:35858-36968 -
- HCN73_09395 MBA2916525.1 31750 - 32599 (-) JAATOH010000049.1:37092-37941 -
- HCN73_09400 MBA2916526.1 32800 - 35200 (+) JAATOH010000049.1:38142-40542 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 8 (-) CDS No
- 1 - 1320 (-) CDS No
- 4387 - 6855 (-) CAZyme: GH43_4|GH43_4 Yes
- 7142 - 8740 (+) other Yes
- 8759 - 9487 (+) other Yes
- 9628 - 10722 (+) TF: DBD-Pfam|GntR,DBD-SUPERFAMILY|0043758 Yes
- 10861 - 11820 (-) other Yes
- 11804 - 13675 (-) CAZyme: GH2|GH2 Yes
araA 13847 - 15274 (+) other Yes
- 15456 - 17120 (-) CAZyme: GH43_4|GH43_4 Yes
- 17297 - 19243 (+) CAZyme: GH127| GH127 Yes
- 19246 - 20694 (+) CAZyme: GH51|GH51 Yes
- 20787 - 21416 (-) other Yes
- 21661 - 23019 (+) TC: gnl|TC-DB|P94528|3.A.1.1.34 Yes
- 23093 - 24010 (+) TC: gnl|TC-DB|P94529|3.A.1.1.34 Yes
- 24027 - 24875 (+) TC: gnl|TC-DB|P94530|3.A.1.1.34 Yes
- 24883 - 25554 (+) other Yes
- 25634 - 26578 (+) CAZyme: GH43_26|GH43_26 Yes
- 26598 - 28091 (+) CAZyme: GH51|GH51 Yes
- 28102 - 29124 (+) other Yes
- 29190 - 30458 (+) CAZyme: GH27|GH27 Yes
- 30517 - 31626 (-) TC: gnl|TC-DB|Q7WWQ6|3.A.1.1.20 Yes
- 31751 - 32599 (-) TF: DBD-Pfam|HTH_8,DBD-SUPERFAMILY|0037176 No
- 32801 - 35200 (+) CDS No

PUL ID

PUL0626

PubMed

33119797, Appl Microbiol Biotechnol. 2020 Dec;104(23):10091-10103. doi: 10.1007/s00253-020-10979-0. Epub 2020 Oct 29.

Title

Characterization of two extracellular arabinanases in Lactobacillus crispatus.

Author

Li Q, Ganzle MG

Abstract

Extracellular glycosyl hydrolases are uncommon in lactobacilli and include amylases and fructosidases mediating starch and fructan utilization, respectively. Extracellular arabinanases have not been described in lactobacilli. This study is aimed at identifying the function of an arabinan utilization operon in Lactobacillus crispatus DSM29598 and at characterizing two putative extracellular arabinanases that are located on that operon. The arabinan utilization operon of L. crispatus DSM29598 encodes enzymes for degradation of arabinan, alpha-galactosidases, beta-galactosidases, and enzymes and for utilization of arabinose including phosphoketolase. The two putative extracellular arabinanases, AbnA and AbnB, are homologous to family GH43 endo-arabinanases. In Lactobacillaceae, homologs of these enzymes were identified exclusively in vertebrate-adapted species of the genus Lactobacillus. L. crispatus grew with arabinan from sugar beet pectin as sole carbon source, indicating extracellular arabinanase activity, and produced lactate and acetate, indicating metabolism via the phosphoketolase pathway. The two arabinanases AbnA and AbnB were heterologously expressed and purified by affinity chromatography. AbnA hydrolyzed linear and branched arabinan, while AbnB hydrolyzed only linear arabinan. The optimum pH for AbnA and AbnB was 6 and 7.5, respectively; 40 degrees C was the optimum temperature for both enzymes. The application of arabinan degrading L. crispatus as probiotic or as synbiotic with pectins may improve the production of short-chain fatty acids from pectin to benefit host health. KEY POINTS: * An arabinan utilization operon in L. crispatus encodes two extracellular arabinanases. * The same operon also encodes metabolic genes for arabinose conversion. * In Lactobacillaceae, extracellular arabinanases are exclusive to Lactobacillus species.