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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0620

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Characterization method

RNA-Seq

Genomic accession number

JABKKE010000016.1

Nucelotide position range

24466-52842

Substrate

xylan

Loci

HPS55_09945-HPS55_09990

Species

Prevotella sp. PROD/2736291

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HPS55_09945 NPE14635.1 0 - 3660 (+) JABKKE010000016.1:24466-28126 -
- HPS55_09950 NPE14636.1 4472 - 4709 (+) JABKKE010000016.1:28938-29175 -
- HPS55_09955 NPE14637.1 5109 - 8640 (+) JABKKE010000016.1:29575-33106 -
- HPS55_09960 NPE14638.1 8780 - 13073 (+) JABKKE010000016.1:33246-37539 -
- HPS55_09965 NPE14639.1 13117 - 15775 (+) JABKKE010000016.1:37583-40241 -
- HPS55_09970 NPE14640.1 15784 - 19153 (+) JABKKE010000016.1:40250-43619 -
- HPS55_09975 NPE14641.1 19185 - 21009 (+) JABKKE010000016.1:43651-45475 -
- HPS55_09980 NPE14642.1 21058 - 23149 (+) JABKKE010000016.1:45524-47615 -
- HPS55_09985 NPE14643.1 23320 - 25099 (+) JABKKE010000016.1:47786-49565 -
- HPS55_09990 NPE14644.1 25134 - 28377 (+) JABKKE010000016.1:49600-52843 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 3660 (+) CAZyme: GH128| GH128 Yes
- 4473 - 4709 (+) other Yes
- 5110 - 8640 (+) other Yes
- 8781 - 13073 (+) CAZyme: GH43_19|GH43_19| GH51|GH51 Yes
- 13118 - 15775 (+) other Yes
- 15785 - 19153 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 19186 - 21009 (+) CDS No
- 21059 - 23149 (+) CDS No
- 23321 - 25099 (+) CDS No
- 25135 - 28377 (+) CDS No

PUL ID

PUL0620

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Title

Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Author

Galvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E, Strowig T

Abstract

Prevotella spp. are a dominant bacterial genus within the human gut. Multiple Prevotella spp. co-exist in some individuals, particularly those consuming plant-based diets. Additionally, Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates. To investigate the relationship between Prevotella competition and diet, we isolated Prevotella species from the mouse gut, analyzed their genomes and transcriptomes in vivo, and performed competition experiments between species in mice. Diverse dominant Prevotella species compete for similar metabolic niches in vivo, which is linked to the upregulation of specific polysaccharide utilization loci (PULs). Complex plant-derived polysaccharides are required for Prevotella spp. expansion, with arabinoxylans having a prominent impact on species abundance. The most dominant Prevotella species encodes a specific tandem-repeat trsusC/D PUL that enables arabinoxylan utilization and is conserved in human Prevotella copri strains, particularly among those consuming a vegan diet. These findings suggest that efficient (arabino)xylan-utilization is a factor contributing to Prevotella dominance.