Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0004 enzyme activity assay, substrate binding assay glucose, cellobiose, maltose uncultured bacterium 26827771
A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase. Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15.
2016 Mar degradation 2 1 GH1
PUL0015 microarray cellobiose Lactococcus lactis 28970222
Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363. Appl Environ Microbiol. 2017 Nov 16;83(23). pii: AEM.01279-17. doi: 10.1128/AEM.01279-17. Print 2017 Dec 1.
2017 Dec 1 degradation 4 1 GH9
PUL0016 microarray cellobiose Lactococcus lactis 28970222
Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363. Appl Environ Microbiol. 2017 Nov 16;83(23). pii: AEM.01279-17. doi: 10.1128/AEM.01279-17. Print 2017 Dec 1.
2017 Dec 1 degradation 5 1 GH1
PUL0017 qRT-PCR, Western Blot, isothermal titration calorimetry cellobiose Ruminiclostridium cellulolyticum 29093754
A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis. Biotechnol Biofuels. 2017 Oct 30;10:250. doi: 10.1186/s13068-017-0933-7. eCollection 2017.
2017 degradation 9 1 GH94
PUL0019 enzyme activity assay, Northern Blot lichenan, cellobiose, beta-glucoside Bacillus subtilis 8990303
Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis. J Bacteriol. 1997 Jan;179(2):496-506. doi: 10.1128/jb.179.2.496-506.1997.
1997 Jan degradation 6 1 GH4
PUL0022 RT-PCR, gene deletion mutant and growth assay, enzyme activity assay cellobiose Bacillus coagulans 30519284
Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018.
2018 degradation 6 1 GH1
PUL0023 RT-PCR, gene deletion mutant and growth assay, enzyme activity assay cellobiose Bacillus coagulans 30519284
Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018.
2018 degradation 5 1 GH1
PUL0041 Southern Blot, enzyme activity assay cellobiose Klebsiella oxytoca 9023916
Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides. Appl Environ Microbiol. 1997 Feb;63(2):355-63. doi: 10.1128/AEM.63.2.355-363.1997.
1997 Feb degradation 3 1 GH1
PUL0186 gene deletion mutant and growth assay cellobiose Streptococcus pneumoniae 17028271
The two-component regulatory system TCS08 is involved in cellobiose metabolism of Streptococcus pneumoniae R6. J Bacteriol. 2007 Feb;189(4):1342-50. doi: 10.1128/JB.01170-06. Epub 2006 Oct 6.
2007 Feb degradation 7 1 GH1
PUL0195 RT-PCR cellobiose Clostridium acetobutylicum 26691835
PTS regulation domain-containing transcriptional activator CelR and sigma factor sigma(54) control cellobiose utilization in Clostridium acetobutylicum. Mol Microbiol. 2016 Apr;100(2):289-302. doi: 10.1111/mmi.13316. Epub 2016 Feb 9.
2016 Apr degradation 5 1 GH1
PUL0331 carbon utilization assay cellobiose Aliivibrio fischeri 18487409
Identification of a cellobiose utilization gene cluster with cryptic beta-galactosidase activity in Vibrio fischeri. Appl Environ Microbiol. 2008 Jul;74(13):4059-69. doi: 10.1128/AEM.00190-08. Epub 2008 May 16.
2008 Jul degradation 6 1 GH1
PUL0413 enzyme activity assay, reducing-sugar assay cellobiose uncultured bacterium contig00059 30116044
Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite. ISME J. 2019 Jan;13(1):104-117. doi: 10.1038/s41396-018-0255-1. Epub 2018 Aug 16.
2019 Jan degradation 31 1 GH44
PUL0423 clone and expression, enzyme activity assay cellobiose Thermotoga neapolitana 10960102
Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana. J Bacteriol. 2000 Sep;182(18):5172-9. doi: 10.1128/jb.182.18.5172-5179.2000.
2000 Sep degradation 3 2 GH94
PUL0475 clone and expression, gene deletion mutant and growth assay cellobiose, cellotriose Streptomyces reticuli 10347054
Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli. Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999.
1999 Jun degradation 7 1 GH18, CBM2
PUL0538 RNA-Seq galactomannan, glucomannan, cellobiose, xyloglucan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 15 7 GH130, GH26, GH26, GH5_2, GH5, GH5_7, CE7, GH3
PUL0543 RNA-Seq cellobiose, beta-glucan, glucosamine Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 6 3 GH30, GH30_3, GH2, CBM57, GH88
PUL0546 RNA-Seq arabinogalactan, cellobiose Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 8 4 GH2, GH30_4, GH51
PUL0551 RNA-Seq cellobiose, beta-glucan, glucomannan, laminarin Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 6 2 GH16, GH3
PUL0570 clone and expression, enzyme activity assay cellobiose Corynebacterium glutamicum 12777497
A single V317A or V317M substitution in Enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose. Microbiology (Reading). 2003 Jun;149(Pt 6):1569-1580. doi: 10.1099/mic.0.26053-0.
2003 Jun degradation 3 1 GH1
PUL0573 enzyme activity assay, electrophoretic mobility shift assay, RT-PCR, qRT-PCR cellobiose, cellulose, cellooligosaccharide Streptomyces griseus 19648249
CebR as a master regulator for cellulose/cellooligosaccharide catabolism affects morphological development in Streptomyces griseus. J Bacteriol. 2009 Oct;191(19):5930-40. doi: 10.1128/JB.00703-09. Epub 2009 Jul 31.
2009 Oct degradation 5 1 GH1
PUL0577 SDS-PAGE, enzyme activity assay chitin, chitobiose, cellobiose Photobacterium profundum 21098515
Elucidation of exo-beta-D-glucosaminidase activity of a family 9 glycoside hydrolase (PBPRA0520) from Photobacterium profundum SS9. Glycobiology. 2011 Apr;21(4):503-11. doi: 10.1093/glycob/cwq191. Epub 2010 Nov 22.
2011 Apr degradation 11 3 GH94, GH20, GH9
PUL0583 enzyme activity assay, gene deletion mutant and growth assay cellobiose Geobacillus stearothermophilus 8407820
Cloning and sequencing of a cellobiose phosphotransferase system operon from Bacillus stearothermophilus XL-65-6 and functional expression in Escherichia coli. J Bacteriol. 1993 Oct;175(20):6441-50. doi: 10.1128/jb.175.20.6441-6450.1993.
1993 Oct degradation 5 1 CE0
PUL0585 microarray, gene deletion mutant and growth assay, beta-galactosidase assays cellobiose Streptococcus pneumoniae 21778207
CelR-mediated activation of the cellobiose-utilization gene cluster in Streptococcus pneumoniae. Microbiology (Reading). 2011 Oct;157(Pt 10):2854-2861. doi: 10.1099/mic.0.051359-0. Epub 2011 Jul 21.
2011 Oct degradation 7 1 GH1