dbCAN-PUL

PUL ID	PUL0004
PubMed	26827771, Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15.
Characterization method	enzyme activity assay,substrate binding assay
Genomic accession number	KM624528.1
Nucelotide position range	1-3311
Substrate	glucose,cellobiose,maltose
Loci	glu1392,glu1923
Species	uncultured bacterium/77133
Degradation or Biosynthesis	degradation

PUL ID	PUL0004
PubMed	26827771, Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15.
Title	A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase.
Author	Gao G, Wang A, Gong BL, Li QQ, Liu YH, He ZM, Li G
Abstract	Functional screening of a metagenomic library of termite hindgut identified an overlapping gene cluster encoding the phosphotransferase system (PTS) components, which consisted of a glucoside specific PTS enzyme II gene (glu1923) and a glycoside hydrolase gene (glu1392). Hydrolytic experiments revealed that the combined effect of Glu1923 and Glu1392 was responsible for the utilization of glucosidic substrates in recombinant Escherichia coli (E. coli) strains. Yeast two hybrid analysis suggested that there was an interaction between Glu1923 and Glu1392, and the domain EIIA of Glu1923 played an important role for the interaction. In addition, the hydrolase Glu1392 displayed hydrolysis ability toward cellobiose and maltose, and had a very high tolerance to glucose with a Ki value of 2.25M. These properties make Glu1392 an interesting candidate in biotechnology applications after further study.