PUL ID

PUL0019

PubMed

8990303, J Bacteriol. 1997 Jan;179(2):496-506. doi: 10.1128/jb.179.2.496-506.1997.

Characterization method

enzyme activity assay, Northern Blot

Genomic accession number

Z49992

Nucelotide position range

186-6736

Substrate

lichenan,cellobiose,beta-glucoside

Loci

licRBCAH

Species

Bacillus subtilis/1423

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

celR - CAA90284.1 186 - 2112 (+) Z49992.1:372-2298 -
celA - CAA90285.1 2239 - 2548 (+) Z49992.1:2425-2734 -
celB - CAA90286.1 2563 - 3922 (+) Z49992.1:2749-4108 -
celC - CAA90287.1 3940 - 4273 (+) Z49992.1:4126-4459 -
celD - CAA90288.1 4269 - 5598 (+) Z49992.1:4455-5784 -
ywaA - CAA90289.1 5631 - 6736 (-) Z49992.1:5817-6922 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 187 - 2112 (+) TF: DBD-Pfam|HTH_11,DBD-Pfam|Mga,DBD-SUPERFAMILY|0039384,DBD-SUPERFAMILY|0039384 No
- 2240 - 2548 (+) TC: gnl|TC-DB|P46318|4.A.3.2.2 Yes
- 2564 - 3922 (+) TC: gnl|TC-DB|P46317|4.A.3.2.2 Yes
- 3941 - 4273 (+) TC: gnl|TC-DB|P46319|4.A.3.2.2 Yes
- 4270 - 5598 (+) CAZyme: GH4 Yes
- 5632 - 6723 (-) CDS No

PUL ID

PUL0019

PubMed

8990303, J Bacteriol. 1997 Jan;179(2):496-506. doi: 10.1128/jb.179.2.496-506.1997.

Title

Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis.

Author

Tobisch S, Glaser P, Kruger S, Hecker M

Abstract

A new catabolic system in Bacillus subtilis involved in utilization of beta-glucosidic compounds has been investigated. It consists of five genes encoding phosphotransferase system (PTS) enzyme II (licB and licC) and enzyme IIA (licA), a presumed 6-phospho-beta-glucosidase (licH), as well as a putative regulator protein (licR). The genes map around 334 degrees of the B. subtilis chromosome, and their products are involved in the uptake and utilization of lichenan degradation products. These five genes are organized in two transcriptional units. A weak promoter precedes gene licR, and transcription is obviously terminated at a secondary structure immediately downstream of the reading frame, as shown by Northern RNA blot analysis. Genes licB, licC, licA, and licH constitute an operon. Initiation of transcription at the promoter in front of this operon presumably requires activation by the gene product of licR. The LicR protein shows an unusual domain structure, i.e., similarities to (i) the conserved transcriptional antiterminator BgIG family signature and (ii) PTS enzyme II. Using RNA techniques and transcriptional lacZ fusions, we have shown that the expression of the licBCAH operon is inducible by products of lichenan hydrolysis, lichenan and cellobiose. The presence of excess glucose prevents the induction of this operon, indicating the control by carbon catabolite repression. Moreover, the expression of the operon requires the general PTS components and seems to be negatively controlled by the specific lic PTS enzymes.