Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0049 fosmid library screen beta-glucan, galactomannan feces metagenome 29601586
Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018.
2018 degradation 29 6 GH43_17, GH26, GH16
PUL0050 fosmid library screen cellulose, galactomannan feces metagenome 29601586
Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018.
2018 degradation 20 7 GH5_4, GH5, GH26, GH130, GH3, GH94
PUL0085 label-free quantitative proteomics, functional enrichment analysis, enzyme activity assay lignocellulose Ruminiclostridium papyrosolvens 31338125
Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation. Biotechnol Biofuels. 2019 Jul 15;12:183. doi: 10.1186/s13068-019-1522-8. eCollection 2019.
2019 degradation 3 3 CBM35, GH26, GH9, CBM3, GH9
PUL0099 RNA-Seq, substrate binding assay, enzyme activity assay, mass spectrometry beta-mannan Roseburia intestinalis 30796211
The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.
2019 Feb 22 degradation 3 2 CBM27, GH26, CBM23, GH3, GH3
PUL0129 enzyme activity assay mannan gut metagenome 30356154
Interspecies cross-feeding orchestrates carbon degradation in the rumen ecosystem. Nat Microbiol. 2018 Nov;3(11):1274-1284. doi: 10.1038/s41564-018-0225-4. Epub 2018 Oct 24.
2018 Nov degradation 12 6 GH5, GH5_4, GH26, GH26, GH5, GH5_4, CE7, CE7, GH26, GH130
PUL0164 mass spectrometry, sequence homology analysis, differential gene expression beta-mannan Leeuwenhoekiella sp. MAR_2009_132 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 19 9 CE2, GH3, GH5_7, GH26, GH130, GH26, GH5_2, GH5, GH27, GH9, GH26
PUL0165 mass spectrometry, sequence homology analysis, differential gene expression beta-mannan Salegentibacter sp. Hel_I_6 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 17 6 GH9, GH27, GH5, GH5_2, GH26, GH130, GH26
PUL0167 mass spectrometry, sequence homology analysis beta-mannan Bacteroides ovatus 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 12 4 GH130, GH36, GH26, GH26
PUL0178 enzyme activity assay, enzyme specificity assay, substrate specificity assay galactomannan Bacteroides ovatus 27288925, 22205877
A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2016 Jul,2011 Dec degradation 14 4 GH130, GH36, GH26, GH26
PUL0179 enzyme activity assay, enzyme specificity assay, substrate specificity assay galactomannan Bacteroides ovatus 27288925
A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28.
2016 Jul degradation 15 3 CE7, GH130, GH26, GH26
PUL0222 fosmid library screen cellulose uncultured bacterium Contig196 24223817
Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013.
2013 degradation 7 3 GH5, GH5_7, GH5_4, GH26
PUL0238 Northern Blot glucomannan Bacillus subtilis 18177310
Glucomannan utilization operon of Bacillus subtilis. FEMS Microbiol Lett. 2008 Feb;279(1):103-9. doi: 10.1111/j.1574-6968.2007.01018.x.
2008 Feb degradation 8 1 GH26
PUL0239 fosmid library screen, sequence homology analysis cellulose Prevotella sp. Sc00026 24448980
Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
2014 Mar degradation 18 10 GH26, GH5_4, GH5_7, GH130, GH26, CE7, GH36, GH3
PUL0240 fosmid library screen, sequence homology analysis cellulose Prevotella sp. Sc00028 24448980
Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
2014 Mar degradation 11 5 GH31, GH9, GH26
PUL0242 fosmid library screen, sequence homology analysis cellulose Prevotella sp. Sc00044 24448980
Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
2014 Mar degradation 9 3 GH31, CBM72, GH5_4, GH26
PUL0408 enzyme activity assay, thin layer chromatography mannan Bacteroides fragilis 24217874
The mannobiose-forming exo-mannanase involved in a new mannan catabolic pathway in Bacteroides fragilis. Arch Microbiol. 2014 Jan;196(1):17-23. doi: 10.1007/s00203-013-0938-y. Epub 2013 Nov 12.
2014 Jan degradation 4 2 GH26, GH130
PUL0488 growth assay beta-mannan Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 14 7 GH5, GH5_2, GH27, GH26, GH130, GH26, GH5_7, GH97
PUL0538 RNA-Seq galactomannan, glucomannan, cellobiose, xyloglucan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 15 7 GH130, GH26, GH26, GH5_2, GH5, GH5_7, CE7, GH3
PUL0547 RNA-Seq mannose Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 11 4 GH130, GH26, GH2, GH3
PUL0565 microarray, qPCR galactomannan, glucomannan Bacteroides ovatus 22205877, 27872187
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus: ENZYME SYNERGY AND CRYSTAL STRUCTURE OF A beta-MANNANASE. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. J Biol Chem. 2017 Jan 6;292(1):229-243. doi: 10.1074/jbc.M116.746438. Epub 2016 Nov 21.
2011 Dec,2017 Jan 6 degradation 10 4 GH130, GH36, GH26, GH26