dbCAN-PUL

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.

PUL ID	PUL0099
PubMed	30796211, Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.
Characterization method	RNA-Seq,substrate binding assay,enzyme activity assay,mass spectrometry
Genomic accession number	NZ_GG692727.1
Nucelotide position range	64567-74095
Substrate	beta-mannan
Loci	ROSINTL182_RS11930-ROSINTL182_RS11940
Species	Roseburia intestinalis/166486
Degradation or Biosynthesis	degradation

Gene Name	Locus Tag	Protein ID	Gene Position	GenBank Contig Range	EC Number
-	ROSINTL182_RS11930	WP_044999186.1	0 - 3927 (-)	NZ_GG692727.1:64567-68494	-
-	ROSINTL182_RS11935	WP_006857766.1	4119 - 6924 (-)	NZ_GG692727.1:68686-71491	-
-	ROSINTL182_RS11940	WP_006857767.1	7111 - 9529 (-)	NZ_GG692727.1:71678-74096	-

Cluster number	0
Gene name	Gene position	Gene type	Found by CGCFinder?
-	1 - 3927 (-)	CAZyme: CBM27\|GH26\|CBM23	No
-	4120 - 6924 (-)	CAZyme: GH3	No
-	7112 - 9529 (-)	CAZyme: GH3	No

PUL ID	PUL0099
PubMed	30796211, Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.
Title	The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans.
Author	La Rosa SL, Leth ML, Michalak L, Hansen ME, Pudlo NA, Glowacki R, Pereira G, Workman CT, Arntzen MO, Pope PB, Martens EC, Hachem MA, Westereng B
Abstract	beta-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of beta-mannans. We combine multi-"omic" analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in beta-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available beta-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment. In murine experiments, beta-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using beta-mannan-based therapeutic interventions.

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