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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0164

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Characterization method

mass spectrometry,sequence homology analysis,differential gene expression

Genomic accession number

NZ_JHZY01000004.1

Nucelotide position range

1690359-1719497

Substrate

beta-mannan

Loci

P164_RS15845-P164_RS15935

Species

Leeuwenhoekiella sp. MAR_2009_132/1392489

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- P164_RS15845 WP_028377306.1 0 - 1083 (+) NZ_JHZY01000004.1:1690359-1691442 -
- P164_RS15850 WP_028377307.1 1103 - 2486 (+) NZ_JHZY01000004.1:1691462-1692845 -
bglX P164_RS15855 WP_081817411.1 2486 - 4709 (-) NZ_JHZY01000004.1:1692845-1695068 3.2.1.21
- P164_RS15860 WP_117434339.1 4803 - 6111 (-) NZ_JHZY01000004.1:1695162-1696470 -
- P164_RS15865 WP_028377310.1 6085 - 7333 (-) NZ_JHZY01000004.1:1696444-1697692 -
- P164_RS15870 WP_051621379.1 7340 - 8531 (-) NZ_JHZY01000004.1:1697699-1698890 -
- P164_RS15875 WP_028377312.1 8517 - 9723 (-) NZ_JHZY01000004.1:1698876-1700082 -
- P164_RS15880 WP_028377313.1 9725 - 11564 (-) NZ_JHZY01000004.1:1700084-1701923 -
- P164_RS15885 WP_159106034.1 11577 - 12615 (-) NZ_JHZY01000004.1:1701936-1702974 -
- P164_RS15890 WP_028377315.1 12912 - 13785 (+) NZ_JHZY01000004.1:1703271-1704144 -
- P164_RS15895 WP_035900160.1 13816 - 14773 (+) NZ_JHZY01000004.1:1704175-1705132 -
- P164_RS15900 WP_051621381.1 15071 - 18257 (+) NZ_JHZY01000004.1:1705430-1708616 -
- P164_RS15905 WP_028377317.1 18262 - 19819 (+) NZ_JHZY01000004.1:1708621-1710178 -
- P164_RS15910 WP_051621382.1 19831 - 20884 (+) NZ_JHZY01000004.1:1710190-1711243 -
- P164_RS15915 WP_028377318.1 20965 - 22177 (+) NZ_JHZY01000004.1:1711324-1712536 -
- P164_RS15920 WP_028377319.1 22252 - 23590 (-) NZ_JHZY01000004.1:1712611-1713949 -
- P164_RS15925 WP_028377320.1 23727 - 26223 (+) NZ_JHZY01000004.1:1714086-1716582 -
- P164_RS15930 WP_035899932.1 26219 - 27197 (+) NZ_JHZY01000004.1:1716578-1717556 -
- P164_RS15935 WP_028377321.1 27180 - 29139 (+) NZ_JHZY01000004.1:1717539-1719498 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1083 (+) CAZyme: CE2 Yes
- 1104 - 2486 (+) other Yes
bglX 2487 - 4709 (-) CAZyme: GH3 Yes
- 4804 - 6111 (-) CAZyme: GH5_7 Yes
- 6086 - 7333 (-) CAZyme: GH26 Yes
- 7341 - 8531 (-) other Yes
- 8518 - 9723 (-) CAZyme: GH130 Yes
- 9726 - 11564 (-) TC: gnl|TC-DB|A1S2A8|2.A.21.3.8 Yes
- 11578 - 12615 (-) CAZyme: GH26 Yes
- 12913 - 13785 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 Yes
- 13817 - 14773 (+) CAZyme: GH5_2|GH5 Yes
- 15072 - 18257 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 18263 - 19819 (+) TC: gnl|TC-DB|C6Y217|8.A.46.1.3 Yes
- 19832 - 20884 (+) other Yes
- 20966 - 22177 (+) CAZyme: GH27 Yes
- 22253 - 23590 (-) other Yes
- 23728 - 26223 (+) CAZyme: GH9 Yes
- 26220 - 27197 (+) CAZyme: GH26 Yes
- 27181 - 29139 (+) CDS No

PUL ID

PUL0164

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Title

Alpha- and beta-mannan utilization by marine Bacteroidetes.

Author

Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avci B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H

Abstract

Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest alpha- and beta-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for alpha- and beta-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.