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PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0050

PubMed

29601586, PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018.

Characterization method

fosmid library screen

Genomic accession number

LT796703

Nucelotide position range

70-23956

Substrate

cellulose,galactomannan

Loci

SJX74200.1-SJX74219.1

Species

feces metagenome/1861841

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - SJX74200.1 70 - 1657 (+) LT796703.1:140-1727 -
- - SJX74201.1 1669 - 3214 (+) LT796703.1:1739-3284 -
- - SJX74202.1 3225 - 4320 (+) LT796703.1:3295-4390 -
- - SJX74203.1 4341 - 5499 (+) LT796703.1:4411-5569 -
- - SJX74204.1 5577 - 6921 (+) LT796703.1:5647-6991 -
- - SJX74205.1 7071 - 7254 (+) LT796703.1:7141-7324 3.2.1.22
- - SJX74206.1 7243 - 9118 (-) LT796703.1:7313-9188 -
- - SJX74207.1 9332 - 11570 (+) LT796703.1:9402-11640 3.2.1.21
- - SJX74208.1 11658 - 14130 (-) LT796703.1:11728-14200 -
- - SJX74209.1 14221 - 14671 (-) LT796703.1:14291-14741 -
- - SJX74210.1 14674 - 15742 (-) LT796703.1:14744-15812 2.7.7.22
- - SJX74211.1 15797 - 16025 (-) LT796703.1:15867-16095 -
- - SJX74212.1 16132 - 16603 (-) LT796703.1:16202-16673 -
- - SJX74213.1 16628 - 19214 (-) LT796703.1:16698-19284 -
- - SJX74214.1 19215 - 20013 (-) LT796703.1:19285-20083 -
- - SJX74215.1 20197 - 21370 (+) LT796703.1:20267-21440 -
- - SJX74216.1 21562 - 22186 (+) LT796703.1:21632-22256 -
- - SJX74217.1 22189 - 22816 (+) LT796703.1:22259-22886 -
- - SJX74218.1 22828 - 23119 (+) LT796703.1:22898-23189 -
- - SJX74219.1 23131 - 23956 (+) LT796703.1:23201-24026 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 71 - 1657 (+) CDS No
- 1670 - 3214 (+) CAZyme: GH5_4|GH5 Yes
- 3226 - 4320 (+) CAZyme: GH26 Yes
- 4342 - 5499 (+) CAZyme: GH130 Yes
- 5578 - 6921 (+) TC: gnl|TC-DB|P75683|2.A.2.3.5 Yes
- 7072 - 7254 (+) other Yes
- 7244 - 9118 (-) other Yes
- 9333 - 11570 (+) CAZyme: GH3 Yes
- 11659 - 14130 (-) CAZyme: GH94 Yes
- 14222 - 14671 (-) CDS No
- 14675 - 15742 (-) CDS No
- 15798 - 16025 (-) CDS No
- 16133 - 16603 (-) CDS No
- 16629 - 19214 (-) CDS No
- 19216 - 20013 (-) CDS No
- 20198 - 21370 (+) CDS No
- 21563 - 22186 (+) CDS No
- 22190 - 22816 (+) CDS No
- 22829 - 23119 (+) CDS No
- 23132 - 23956 (+) CDS No

PUL ID

PUL0050

PubMed

29601586, PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018.

Title

Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary.

Author

Ameri R, Laville E, Potocki-Veronese G, Trabelsi S, Mezghani M, Elgharbi F, Bejar S

Abstract

Dromedaries are capable of digesting plant cell wall with high content of lignocellulose of poor digestibility. Consequently, their intestinal microbiota can be a source of novel carbohydrate-active enzymes (CAZymes). To the best of our knowledge, no data are available describing the biochemical analysis of enzymes in dromedary intestinal microbiota. To investigate new hydrolytic enzymes from the dromedary gut, a fosmid library was constructed using metagenomic DNA from feces of non-domestic adult dromedary camels living in the Tunisian desert. High-throughput functional screening of 13756 clones resulted in 47 hit clones active on a panel of various chromogenic and non-chromogenic glycan substrates. Two of them, harboring multiple activities, were retained for further analysis. Clone 26H3 displayed activity on AZO-CM-cellulose, AZCL Carob galactomannan and Tween 20, while clone 36A23 was active on AZCL carob galactomannan and AZCL barley beta-glucan. The functional annotation of their sequences highlighted original metagenomic loci originating from bacteria of the Bacteroidetes/Chlorobi group, involved in the metabolization of mannosides and beta-glucans thanks to a complete battery of endo- and exo-acting glycoside hydrolases, esterases, phosphorylases and transporters.