Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0662

PubMed

34339781, Int J Biol Macromol. 2021 Sep 30;187:664-674. doi: 10.1016/j.ijbiomac.2021.07.172. Epub 2021 Jul 31.

Characterization method

thin layer chromatography,liquid chromatography and mass spectrometry,qPCR,clone and expression

Genomic accession number

NZ_DS995531.1

Nucelotide position range

353357-368726

Substrate

β-mannan

Loci

BACDOR_RS18975-BACDOR_RS18935

Species

Phocaeicola dorei DSM 17855/357276 

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BACDOR_RS18975 WP_032935600.1 0 - 3924 (-) NZ_DS995531.1:353357-357281 -
- BACDOR_RS18965 WP_227235709.1 4236 - 5433 (+) NZ_DS995531.1:357593-358790 -
- BACDOR_RS18960 WP_235778214.1 5504 - 6734 (+) NZ_DS995531.1:358861-360091 -
- BACDOR_RS18955 WP_007831881.1 6768 - 10020 (+) NZ_DS995531.1:360125-363377 -
- BACDOR_RS18950 WP_007831882.1 10065 - 11850 (+) NZ_DS995531.1:363422-365207 -
- BACDOR_RS18945 WP_007831884.1 11895 - 13068 (+) NZ_DS995531.1:365252-366425 -
- BACDOR_RS18940 WP_007831886.1 13071 - 14172 (+) NZ_DS995531.1:366428-367529 -
- BACDOR_RS18935 WP_007831887.1 14197 - 15370 (+) NZ_DS995531.1:367554-368727 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 3924 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607,DBD-SUPERFAMILY|0035607 No
- 4237 - 5433 (+) CAZyme: GH5_7| GH5_7 Yes
- 5505 - 6734 (+) CAZyme: CE7| CE7 Yes
- 6769 - 10020 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 10066 - 11850 (+) TC: gnl|TC-DB|G0IYY9|8.A.46.1.6 Yes
- 11896 - 13068 (+) other Yes
- 13072 - 14172 (+) CAZyme: GH26| GH26 Yes
- 14198 - 15370 (+) CAZyme: GH130|GH130 Yes

PUL ID

PUL0662

PubMed

34339781, Int J Biol Macromol. 2021 Sep 30;187:664-674. doi: 10.1016/j.ijbiomac.2021.07.172. Epub 2021 Jul 31.

Title

BdPUL12 depolymerizes beta-mannan-like glycans into mannooligosaccharides and mannose, which serve as carbon sources for Bacteroides dorei and gut probiotics.

Author

Gao G, Cao J, Mi L, Feng D, Deng Q, Sun X, Zhang H, Wang Q, Wang J

Abstract

Symbiotic bacteria, including members of the Bacteroides genus, are known to digest dietary fibers in the gastrointestinal tract. The metabolism of complex carbohydrates is restricted to a specified subset of species and is likely orchestrated by polysaccharide utilization loci (PULs) in these microorganisms. beta-Mannans are plant cell wall polysaccharides that are commonly found in human nutrients. Here, we report the structural basis of a PUL cluster, BdPUL12, which controls beta-mannan-like glycan catabolism in Bacteroides dorei. Detailed biochemical characterization and targeted gene disruption studies demonstrated that a key glycoside hydrolase, BdP12GH26, performs the initial attack on galactomannan or glucomannan likely via an endo-acting mode, generating mannooligosaccharides and mannose. Importantly, coculture assays showed that the B. dorei promoted the proliferation of Lactobacillus helveticus and Bifidobacterium adolescentis, likely by sharing mannooligosaccharides and mannose with these gut probiotics. Our findings provide new insights into carbohydrate metabolism in gut-inhabiting bacteria and lay a foundation for novel probiotic development.