PUL ID

PUL0239

PubMed

24448980, J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.

Characterization method

fosmid library screen, sequence homology analysis

Genomic accession number

JX424618

Nucelotide position range

10820-37418

Substrate

cellulose

Loci

AGH13958.1-AGH13973.1

Species

Prevotella sp. Sc00026/1231727

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - AGH13958.1 0 - 2331 (-) JX424618.1:10820-13151 -
- - AGH13959.1 2327 - 2777 (-) JX424618.1:13147-13597 -
- - AGH13960.1 2853 - 4818 (-) JX424618.1:13673-15638 -
- - AGH13961.1 4839 - 6222 (-) JX424618.1:15659-17042 -
- - AGH13962.1 6242 - 8000 (-) JX424618.1:17062-18820 -
- - AGH13963.1 8019 - 11301 (-) JX424618.1:18839-22121 -
- - AGH13964.1 11767 - 12655 (-) JX424618.1:22587-23475 -
- - AGH13965.1 12651 - 13872 (-) JX424618.1:23471-24692 -
- - AGH13966.1 13874 - 15218 (-) JX424618.1:24694-26038 -
- - AGH13967.1 15239 - 16412 (-) JX424618.1:26059-27232 -
- - AGH13968.1 16419 - 17493 (-) JX424618.1:27239-28313 -
- - AGH13969.1 17489 - 18743 (-) JX424618.1:28309-29563 -
- - AGH13970.1 19154 - 20612 (+) JX424618.1:29974-31432 -
- - AGH13971.1 20640 - 22830 (+) JX424618.1:31460-33650 -
- - AGH13972.1 22912 - 25270 (-) JX424618.1:33732-36090 -
- - AGH13973.1 25312 - 26599 (-) JX424618.1:36132-37419 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2328 (-) CDS No
- 1 - 2331 (-) CAZyme: GH26|GH5_4 Yes
- 2328 - 2777 (-) CAZyme: GH5_7 Yes
- 2331 - 2777 (-) other Yes
- 2854 - 4818 (-) other Yes
- 4840 - 6222 (-) other Yes
- 6243 - 8000 (-) other Yes
- 8020 - 11301 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 11768 - 12655 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 Yes
- 12652 - 13872 (-) other Yes
- 13875 - 15218 (-) TC: gnl|TC-DB|P31435|2.A.2.3.4 Yes
- 15240 - 16412 (-) CAZyme: GH130 Yes
- 16420 - 17493 (-) CAZyme: GH26 Yes
- 17490 - 18743 (-) CAZyme: CE7 Yes
- 19155 - 20612 (+) other Yes
- 20641 - 22830 (+) CAZyme: GH36 Yes
- 22913 - 25270 (-) CAZyme: GH3 Yes
- 25313 - 26599 (-) CDS No

PUL ID

PUL0239

PubMed

24448980, J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.

Title

Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes.

Author

Rosewarne CP, Pope PB, Cheung JL, Morrison M

Abstract

Several unique Sus-like polysaccharide utilization loci (PULs) were identified from bacteria resident in bovine rumen microbiomes through functional screening of a fosmid library. The loci were phylogenetically assigned to the genus Prevotella within the phylum Bacteroidetes. These findings were augmented by a bioinformatic re-evaluation of ruminal Prevotella genomes, revealing additional loci not previously reported in the literature. Analysis of Bacteroidales-affiliated genomes reconstructed from a bovine rumen metagenome in a previous study further expanded the diversity of Sus-like PULs resident in this microbiome. Our findings suggest that Sus-like systems represent an important mechanism for degradation of a range of plant-derived glycans in ruminants.