| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0011 | enzyme activity assay | cellulose | Ruminiclostridium cellulolyticum | 8936327 Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli. Microbiology (Reading). 1996 Apr;142 ( Pt 4):1013-1023. doi: 10.1099/00221287-142-4-1013. |
1996 Apr | degradation | 4 | 4 | GH48, GH8, CBM3, GH9, CBM4, GH9 |
| PUL0040 | Northern Blot, enzyme activity assay | cellulose | Ruminiclostridium cellulolyticum | 12896991, 1398087, 11844767 A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome. Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum. Cel9M, a new family 9 cellulase of the Clostridium cellulolyticum cellulosome. J Bacteriol. 2003 Aug;185(16):4727-33. doi: 10.1128/JB.185.16.4727-4733.2003. Gene. 1992 Sep 21;119(1):17-28. doi: 10.1016/0378-1119(92)90062-t. J Bacteriol. 2002 Mar;184(5):1378-84. doi: 10.1128/JB.184.5.1378-1384.2002. |
2003 Aug,1992 Sep 21,2002 Mar | degradation | 6 | 6 | GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1, GH5 |
| PUL0050 | fosmid library screen | cellulose, galactomannan | feces metagenome | 29601586 Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018. |
2018 | degradation | 20 | 7 | GH5_4, GH5, GH26, GH130, GH3, GH94 |
| PUL0221 | fosmid library screen | cellulose | uncultured bacterium Contig1529 | 24223817 Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013. |
2013 | degradation | 10 | 5 | GH35, GH105, GH3, GH5_4 |
| PUL0222 | fosmid library screen | cellulose | uncultured bacterium Contig196 | 24223817 Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013. |
2013 | degradation | 7 | 3 | GH5, GH5_7, GH5_4, GH26 |
| PUL0224 | RT-PCR, qRT-PCR | cellulose | Ruminiclostridium cellulolyticum | 23418511 A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. PLoS One. 2013;8(2):e56063. doi: 10.1371/journal.pone.0056063. Epub 2013 Feb 13. |
2013 | degradation | 16 | 14 | GH43_16, GH43, CBM6, GH10, CBM6, GH43, GH43_29, CBM6, CBM6, CE1, GH43_10, CBM6, GH62, CBM6, GH43, GH43_29, CBM6, GH146, CBM22, GH27, CBM6, GH59, CBM6, GH2, CBM6, GH62, CBM6, CE6, CBM32, GH95, CBM6, GH30_8, CBM6 |
| PUL0239 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00026 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 18 | 10 | GH26, GH5_4, GH5_7, GH130, GH26, CE7, GH36, GH3 |
| PUL0240 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00028 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 11 | 5 | GH31, GH9, GH26 |
| PUL0241 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00033 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 7 | 3 | GH5, GH5_4, GH36 |
| PUL0242 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00044 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 9 | 3 | GH31, CBM72, GH5_4, GH26 |
| PUL0243 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00066 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 12 | 4 | GH5_38, GH94, GH36 |
| PUL0311 | enzyme activity assay | cellulose | Escherichia coli | 31455320 Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4. BMC Biotechnol. 2019 Aug 27;19(1):63. doi: 10.1186/s12896-019-0556-0. |
2019 Aug 27 | degradation | 4 | 2 | GT2, GH8 |
| PUL0385 | ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high performance anion exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | 20013800 Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2010 Feb | degradation | 12 | 10 | GH48, GH8, GH9, CBM3, CBM4, GH9, CBM30, GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1 |
| PUL0391 | microarray | cellulose, xylan, xyloglucan, pectin, mannan | Caldicellulosiruptor bescii | 21227922 Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725. Nucleic Acids Res. 2011 Apr;39(8):3240-54. doi: 10.1093/nar/gkq1281. Epub 2011 Jan 11. |
2011 Apr | degradation | 15 | 10 | PL11, CBM35, GH12, CBM3, CBM2, CE12, PL3_1, PL3, CBM66, PL9_1, CBM35, CBM66, PL9, CBM22, GH12, CBM0, CBM3, CBM2, CBM1, GH48, GH10, CBM9, GH44, CBM35, GH12, CBM44, CBM3, CBM2, CBM76, CBM10, GH5, GH5_8, CBM35, GH12, CBM5, CBM3, CBM2, GH74, CBM1, GH48, GT39, CBM3, GH9, GH5_8, CBM3, GH5_1, GH5_8, CBM3, GH48, GH9 |
| PUL0573 | enzyme activity assay, electrophoretic mobility shift assay, RT-PCR, qRT-PCR | cellobiose, cellulose, cellooligosaccharide | Streptomyces griseus | 19648249 CebR as a master regulator for cellulose/cellooligosaccharide catabolism affects morphological development in Streptomyces griseus. J Bacteriol. 2009 Oct;191(19):5930-40. doi: 10.1128/JB.00703-09. Epub 2009 Jul 31. |
2009 Oct | degradation | 5 | 1 | GH1 |
| PUL0671 | gene deletion mutant and growth assay, enzyme activity assay, Western blot, qPCR | cellulose | Cytophaga hutchinsonii ATCC 33406 | 34731049 A Type IX Secretion System Substrate Involved in Crystalline Cellulose Degradation by Affecting Crucial Cellulose Binding Proteins in Cytophaga hutchinsonii. Appl Environ Microbiol. 2022 Jan 25;88(2):e0183721. doi: 10.1128/AEM.01837-21. Epub 2021 Nov 3. |
2022 Jan 25 | degradation | 6 | 0 | NA |
Copyright 2020 © YIN LAB, UNL. All rights reserved. Designed by Catie Ausland and Jinfang Zheng. Maintained by Yanbin Yin.