Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0001 RNA-Seq, substrate binding assay, enzyme activity assay, mass spectrometry beta-mannan Roseburia intestinalis 30796211
The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.
2019 Feb 22 degradation 15 7 GH1, CE2, GH130, GH130, GH36, GH113
PUL0038 enzyme activity assay, Southern Blot melibiose, galactose Thermus brockianus 10741834
The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125. Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004.
2000 Feb degradation 8 2 GH42, GH36
PUL0039 enzyme activity assay, Southern Blot melibiose, galactose Thermus thermophilus 10741834
The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125. Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004.
2000 Feb degradation 3 1 GH36
PUL0068 enzyme activity assay, electrophoretic mobility shift assay raffinose Escherichia coli 8277949
Role of two operators in regulating the plasmid-borne raf operon of Escherichia coli. Mol Gen Genet. 1994 Jan;242(1):90-9. doi: 10.1007/BF00277352.
1994 Jan degradation 4 2 GH36, GH32
PUL0088 Southern Blot raffinose, melibiose, isomaltotriose Streptococcus mutans 8764489
The multiple-sugar metabolism (msm) gene cluster of Streptococcus mutans is transcribed as a single operon. FEMS Microbiol Lett. 1996 Jul 1;140(2-3):261-4. doi: 10.1016/0378-1097(96)00191-7.
1996 Jul 1 degradation 8 3 GH36, GH13_18, GH13
PUL0122 enzyme activity assay alpha-galactoside, beta-galactoside, raffinose, melibiose Thermotoga maritima 9741105
Properties of an alpha-galactosidase, and structure of its gene galA, within an alpha-and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima. Syst Appl Microbiol. 1998 Mar;21(1):1-11. doi: 10.1016/s0723-2020(98)80002-7.
1998 Mar degradation 6 3 GH42, GH2, GH36
PUL0138 sequence homology analysis raffinose, melibiose Bifidobacterium animalis subsp. animalis 30306201
Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions. Appl Microbiol Biotechnol. 2018 Dec;102(24):10645-10663. doi: 10.1007/s00253-018-9413-7. Epub 2018 Oct 10.
2018 Dec degradation 7 2 GH36, GH36
PUL0167 mass spectrometry, sequence homology analysis beta-mannan Bacteroides ovatus 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 12 4 GH130, GH36, GH26, GH26
PUL0178 enzyme activity assay, enzyme specificity assay, substrate specificity assay galactomannan Bacteroides ovatus 27288925, 22205877
A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2016 Jul,2011 Dec degradation 14 4 GH130, GH36, GH26, GH26
PUL0180 enzyme activity assay galactomannan Bacteroides fragilis 27288925, 21539815
A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. Biochem Biophys Res Commun. 2011 May 20;408(4):701-6. doi: 10.1016/j.bbrc.2011.04.095. Epub 2011 Apr 24.
2016 Jul,2011 May 20 degradation 19 4 GH36, GH29, GH29
PUL0232 microarray, electrophoretic mobility shift assay raffinose Bifidobacterium breve 24705323
Transcription of two adjacent carbohydrate utilization gene clusters in Bifidobacterium breve UCC2003 is controlled by LacI- and repressor open reading frame kinase (ROK)-type regulators. Appl Environ Microbiol. 2014 Jun;80(12):3604-14. doi: 10.1128/AEM.00130-14.
2014 Jun degradation 6 1 GH36
PUL0233 microarray, electrophoretic mobility shift assay melezitose Bifidobacterium breve 24705323
Transcription of two adjacent carbohydrate utilization gene clusters in Bifidobacterium breve UCC2003 is controlled by LacI- and repressor open reading frame kinase (ROK)-type regulators. Appl Environ Microbiol. 2014 Jun;80(12):3604-14. doi: 10.1128/AEM.00130-14.
2014 Jun degradation 5 2 GH36
PUL0239 fosmid library screen, sequence homology analysis cellulose Prevotella sp. Sc00026 24448980
Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
2014 Mar degradation 18 10 GH26, GH5_4, GH5_7, GH130, GH26, CE7, GH36, GH3
PUL0241 fosmid library screen, sequence homology analysis cellulose Prevotella sp. Sc00033 24448980
Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
2014 Mar degradation 7 3 GH5, GH5_4, GH36
PUL0243 fosmid library screen, sequence homology analysis cellulose Prevotella sp. Sc00066 24448980
Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
2014 Mar degradation 12 4 GH5_38, GH94, GH36
PUL0246 enzyme activity assay, gene deletion mutant and growth assay, Western Blot fucose Streptococcus pneumoniae 24333485
Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae. J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12.
2014 Apr 3 degradation 11 4 GH98, CBM51, GH36, GH36, GH29
PUL0266 enzyme activity assay lactose Halorubrum lacusprofundi 23320757
Cloning, overexpression, purification, and characterization of a polyextremophilic beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi. BMC Biotechnol. 2013 Jan 16;13:3. doi: 10.1186/1472-6750-13-3.
2013 Jan 16 degradation 15 2 GH42, GH36
PUL0276 RT-qPCR isomaltose, panose, raffinose, stachyose, melibiose Bifidobacterium animalis subsp. lactis 23663691
Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04. BMC Genomics. 2013 May 10;14:312. doi: 10.1186/1471-2164-14-312.
2013 May 10 degradation 9 2 GH36, GH36
PUL0374 microarray, qPCR melibiose Dickeya dadantii 19734309
Catabolism of raffinose, sucrose, and melibiose in Erwinia chrysanthemi 3937. J Bacteriol. 2009 Nov;191(22):6960-7. doi: 10.1128/JB.00594-09. Epub 2009 Sep 4.
2009 Nov degradation 3 1 GH36
PUL0486 RT-PCR, sugar utilization assay raffinose Streptococcus pneumoniae 10613841
Regulation of the alpha-galactosidase activity in Streptococcus pneumoniae: characterization of the raffinose utilization system. Genome Res. 1999 Dec;9(12):1189-97. doi: 10.1101/gr.9.12.1189.
1999 Dec degradation 8 2 GH13, GH13_18, GH36
PUL0561 clone and expression, enzyme activity assay alpha-galactoside Lactobacillus plantarum 12406739
Characterization of the melA locus for alpha-galactosidase in Lactobacillus plantarum. Appl Environ Microbiol. 2002 Nov;68(11):5464-71. doi: 10.1128/aem.68.11.5464-5471.2002.
2002 Nov degradation 5 2 GH36, GH2
PUL0565 microarray, qPCR galactomannan, glucomannan Bacteroides ovatus 22205877, 27872187
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus: ENZYME SYNERGY AND CRYSTAL STRUCTURE OF A beta-MANNANASE. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. J Biol Chem. 2017 Jan 6;292(1):229-243. doi: 10.1074/jbc.M116.746438. Epub 2016 Nov 21.
2011 Dec,2017 Jan 6 degradation 10 4 GH130, GH36, GH26, GH26
PUL0575 microarray, growth assay, gene deletion mutant and growth assay raffinose Enterococcus faecium 20946531
A genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source. Environ Microbiol. 2011 Feb;13(2):518-28. doi: 10.1111/j.1462-2920.2010.02355.x. Epub 2010 Oct 15.
2011 Feb degradation 11 2 GH13, GH13_18, GH13_31, GH13, GH36, GH4
PUL0584 microarray melibiose Lactococcus lactis 23530958
Genotype-phenotype matching analysis of 38 Lactococcus lactis strains using random forest methods. BMC Microbiol. 2013 Mar 26;13:68. doi: 10.1186/1471-2180-13-68.
2013 Mar 26 degradation 11 2 GH13, GH13_18, GH36