Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0039

PubMed

10741834, Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004.

Characterization method

enzyme activity assay,Southern Blot

Genomic accession number

AF135399.1

Nucelotide position range

770-5022

Substrate

melibiose,galactose

Loci

aidB-agaT-galT

Species

Thermus thermophilus/274

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - AAD32627.1 770 - 2114 (+) AF135399.1:1540-2884 -
agaT - AAD32628.1 2558 - 3989 (+) AF135399.1:3328-4759 -
galT - AAD32629.1 3972 - 5022 (+) AF135399.1:4742-5792 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 771 - 2114 (+) CDS No
- 2559 - 3989 (+) CAZyme: GH36 No
- 3973 - 5022 (+) CDS No

PUL ID

PUL0039

PubMed

10741834, Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004.

Title

The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125.

Author

Fridjonsson O, Watzlawick H, Mattes R

Abstract

The Thermus thermophilus TH125 alpha-galactosidase gene, agaT, and flanking sequences were cloned in Escherichia coli and sequenced as well as flanking sequences of the previously cloned agaT from Thermus brockianus ITI360. Different structures of putative alpha-galactosidase operons in the two Thermus strains were revealed. Downstream of and overlapping with the alpha-galactosidase genes of both strains, a gene was identified that is similar to the galactose-1-phosphate uridylyltransferase gene (galT) of E. coli and Streptomyces lividans. Upstream of the agaT of T. brockianus ITI360, four open reading frames were observed. The deduced translation products displayed similarity to components of bacterial binding protein-dependent transport systems and a beta-galactosidase. No galactoside utilization genes were identified upstream of agaT in T. thermophilus TH125. The inactivation of the alpha-galactosidase genes of both strains by insertional mutagenesis led to an inability to use melibiose or galactose as a single carbohydrate source. An attempt was made to isolate a gene encoding the enzyme responsible for para-nitrophenyl-(pNP-) beta-galactoside hydrolyzing activity in T. thermophilus TH125. A gene designated bglT was cloned and expressed in E. coli. The inactivation of the bglT gene led to 55% reduction of the pNP-beta-galactoside hydrolyzing activity in the mutant strain in comparison to the wild type.