Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0008 enzyme activity assay fructan Bacteroides thetaiotaomicron 28103254
A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. PLoS One. 2017 Jan 19;12(1):e0169989. doi: 10.1371/journal.pone.0169989. eCollection 2017.
2017 degradation 12 2 GH32, GH32, GH32
PUL0024 enzyme activity assay, qPCR, substrate binding assay kestose uncultured bacterium 31915220
Harvesting of Prebiotic Fructooligosaccharides by Nonbeneficial Human Gut Bacteria. mSphere. 2020 Jan 8;5(1):e00771-19. doi: 10.1128/mSphere.00771-19.
2020 Jan 8 degradation 12 1 GH32
PUL0031 RNA-Seq sucrose Bifidobacterium longum 16523284
A functional analysis of the Bifidobacterium longum cscA and scrP genes in sucrose utilization. Appl Microbiol Biotechnol. 2006 Oct;72(5):975-81. doi: 10.1007/s00253-006-0358-x. Epub 2006 Mar 8.
2006 Oct degradation 3 1 GH32
PUL0068 enzyme activity assay, electrophoretic mobility shift assay raffinose Escherichia coli 8277949
Role of two operators in regulating the plasmid-borne raf operon of Escherichia coli. Mol Gen Genet. 1994 Jan;242(1):90-9. doi: 10.1007/BF00277352.
1994 Jan degradation 4 2 GH36, GH32
PUL0203 qPCR, thin layer chromatography, substrate binding assay exopolysaccharide Bacteroides thetaiotaomicron 25841008
Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3.
2015 Jun 15 degradation 7 3 GH32, GH32, GH32
PUL0219 sugar utilization assay, enzyme activity assay fructooligosaccharide, fructan Lactobacillus paracasei 17644636
Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195. Appl Environ Microbiol. 2007 Sep;73(18):5716-24. doi: 10.1128/AEM.00805-07. Epub 2007 Jul 20.
2007 Sep degradation 7 1 GH32
PUL0277 gene deletion mutant and growth assay, qRT-PCR fructooligosaccharide Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 5 1 GH32
PUL0278 gene deletion mutant and growth assay, qRT-PCR fructooligosaccharide Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 6 1 GH32
PUL0279 gene deletion mutant and growth assay, qRT-PCR fructooligosaccharide Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 6 1 GH32
PUL0312 RT-PCR sucrose Gluconacetobacter diazotrophicus 19139238
Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus. Appl Environ Microbiol. 2009 Mar;75(6):1782-5. doi: 10.1128/AEM.01887-08. Epub 2009 Jan 9.
2009 Mar degradation 2 2 GH68, GH32
PUL0341 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 43 3 GH32, GH91, GH5, GH5_2
PUL0351 enzyme activity assay sucrose Escherichia coli 1435727
Characterization of a chromosomally encoded, non-PTS metabolic pathway for sucrose utilization in Escherichia coli EC3132. Mol Gen Genet. 1992 Oct;235(1):22-32. doi: 10.1007/BF00286177.
1992 Oct degradation 4 1 GH32
PUL0373 enzyme activity assay sucrose Dickeya dadantii 19734309
Catabolism of raffinose, sucrose, and melibiose in Erwinia chrysanthemi 3937. J Bacteriol. 2009 Nov;191(22):6960-7. doi: 10.1128/JB.00594-09. Epub 2009 Sep 4.
2009 Nov degradation 5 1 GH32
PUL0394 microarray fructan, inulin Roseburia inulinivorans 20679207
Substrate-driven gene expression in Roseburia inulinivorans: importance of inducible enzymes in the utilization of inulin and starch. Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1(Suppl 1):4672-9. doi: 10.1073/pnas.1000091107. Epub 2010 Aug 2.
2011 Mar 15 degradation 6 1 GH32
PUL0425 microarray fructan, inulin Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 11 4 GH32, GH91, GH91, GH32
PUL0568 clone and expression, enzyme activity assay, Northern Blot sucrose Clostridium beijerinckii 10411273
The genes controlling sucrose utilization in Clostridium beijerinckii NCIMB 8052 constitute an operon. Microbiology (Reading). 1999 Jun;145 ( Pt 6):1461-1472. doi: 10.1099/13500872-145-6-1461.
1999 Jun degradation 4 1 GH32
PUL0569 clone and expression, enzyme activity assay, Northern Blot levan Bacillus subtilis 11739774
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis. Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413.
2001 Dec degradation 3 1 GH68, GH32
PUL0581 SDS-PAGE, enzyme activity assay inulin Microbulbifer sp. JAM-3301 22286980
Cloning and sequencing of inulinase and beta-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes. Appl Environ Microbiol. 2012 Apr;78(7):2493-5. doi: 10.1128/AEM.07442-11. Epub 2012 Jan 27.
2012 Apr degradation 3 2 GH32, CBM38, GH32
PUL0587 RT-PCR, enzyme activity assay levan, inulin, fructan Prevotella intermedia 23266804
Identification and functional analysis of the gene cluster for fructan utilization in Prevotella intermedia. Gene. 2013 Feb 25;515(2):291-7. doi: 10.1016/j.gene.2012.12.023. Epub 2012 Dec 22.
2013 Feb 25 degradation 3 1 GH32
PUL0604 microarray, qRT-PCR, culureing methods galactomannooligosaccharide Lactobacillus plantarum WCFS1 31703861
Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14.
2020 Apr degradation 6 2 GH32, GH13_31, GH13
PUL0632 recombinant protein expression sucrose, kestose, nystose, inulin Roseburia inulinivorans DSM 16841 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 5 1 CBM66, GH32
PUL0633 recombinant protein expression sucrose, kestose, nystose Roseburia faecis M72 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 5 1 GH32
PUL0634 recombinant protein expression sucrose, kestose, nystose Eubacterium rectale ATCC 33656 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 6 1 GH32
PUL0635 recombinant protein expression sucrose, kestose, nystose, fructooligosaccharide Coprococcus eutactus JCM 31265 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 7 1 CBM38, GH32, CBM66
PUL0636 recombinant protein expression sucrose, kestose, nystose, fructooligosaccharide Coprococcus eutactus JCM 31265 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 8 1 GH32, CBM66
PUL0637 recombinant protein expression sucrose, kestose, nystose Faecalibacterium prausnitzii A2165 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 5 1 GH32
PUL0638 recombinant protein expression sucrose, kestose Anaerostipes hadrus DSM 3319 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 5 1 GH32
PUL0639 recombinant protein expression sucrose, kestose Anaerostipes hadrus DSM 3319 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 4 2 GH32, GH32
PUL0640 recombinant protein expression sucrose, kestose, nystose Anaerostipes hadrus DSM 3319 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 10 1 CBM66, CBM38, GH32
PUL0641 recombinant protein expression sucrose, kestose Anaerostipes caccae L1-92 DSM 14662 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 10 1 GH32
PUL0642 recombinant protein expression sucrose, kestose, nystose Roseburia intestinalis L1-82 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 11 6 GH36, GH13_31, GH13, CBM38, GH32, GH13_18, GH10, CBM86, CBM9, CBM0, CBM22, GH53, CBM61