| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0008 | enzyme activity assay | fructan | Bacteroides thetaiotaomicron | 28103254 A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. PLoS One. 2017 Jan 19;12(1):e0169989. doi: 10.1371/journal.pone.0169989. eCollection 2017. |
2017 | degradation | 12 | 2 | GH32, GH32, GH32 |
| PUL0024 | enzyme activity assay, qPCR, substrate binding assay | kestose | uncultured bacterium | 31915220 Harvesting of Prebiotic Fructooligosaccharides by Nonbeneficial Human Gut Bacteria. mSphere. 2020 Jan 8;5(1):e00771-19. doi: 10.1128/mSphere.00771-19. |
2020 Jan 8 | degradation | 12 | 1 | GH32 |
| PUL0031 | RNA-Seq | sucrose | Bifidobacterium longum | 16523284 A functional analysis of the Bifidobacterium longum cscA and scrP genes in sucrose utilization. Appl Microbiol Biotechnol. 2006 Oct;72(5):975-81. doi: 10.1007/s00253-006-0358-x. Epub 2006 Mar 8. |
2006 Oct | degradation | 3 | 1 | GH32 |
| PUL0068 | enzyme activity assay, electrophoretic mobility shift assay | raffinose | Escherichia coli | 8277949 Role of two operators in regulating the plasmid-borne raf operon of Escherichia coli. Mol Gen Genet. 1994 Jan;242(1):90-9. doi: 10.1007/BF00277352. |
1994 Jan | degradation | 4 | 2 | GH36, GH32 |
| PUL0203 | qPCR, thin layer chromatography, substrate binding assay | exopolysaccharide | Bacteroides thetaiotaomicron | 25841008 Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. |
2015 Jun 15 | degradation | 7 | 3 | GH32, GH32, GH32 |
| PUL0219 | sugar utilization assay, enzyme activity assay | fructooligosaccharide, fructan | Lactobacillus paracasei | 17644636 Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195. Appl Environ Microbiol. 2007 Sep;73(18):5716-24. doi: 10.1128/AEM.00805-07. Epub 2007 Jul 20. |
2007 Sep | degradation | 7 | 1 | GH32 |
| PUL0277 | gene deletion mutant and growth assay, qRT-PCR | fructooligosaccharide | Streptococcus pneumoniae | 23264576 The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21. |
2013 Mar | degradation | 5 | 1 | GH32 |
| PUL0278 | gene deletion mutant and growth assay, qRT-PCR | fructooligosaccharide | Streptococcus pneumoniae | 23264576 The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21. |
2013 Mar | degradation | 6 | 1 | GH32 |
| PUL0279 | gene deletion mutant and growth assay, qRT-PCR | fructooligosaccharide | Streptococcus pneumoniae | 23264576 The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21. |
2013 Mar | degradation | 6 | 1 | GH32 |
| PUL0312 | RT-PCR | sucrose | Gluconacetobacter diazotrophicus | 19139238 Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus. Appl Environ Microbiol. 2009 Mar;75(6):1782-5. doi: 10.1128/AEM.01887-08. Epub 2009 Jan 9. |
2009 Mar | degradation | 2 | 2 | GH68, GH32 |
| PUL0341 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 43 | 3 | GH32, GH91, GH5, GH5_2 |
| PUL0351 | enzyme activity assay | sucrose | Escherichia coli | 1435727 Characterization of a chromosomally encoded, non-PTS metabolic pathway for sucrose utilization in Escherichia coli EC3132. Mol Gen Genet. 1992 Oct;235(1):22-32. doi: 10.1007/BF00286177. |
1992 Oct | degradation | 4 | 1 | GH32 |
| PUL0373 | enzyme activity assay | sucrose | Dickeya dadantii | 19734309 Catabolism of raffinose, sucrose, and melibiose in Erwinia chrysanthemi 3937. J Bacteriol. 2009 Nov;191(22):6960-7. doi: 10.1128/JB.00594-09. Epub 2009 Sep 4. |
2009 Nov | degradation | 5 | 1 | GH32 |
| PUL0394 | microarray | fructan, inulin | Roseburia inulinivorans | 20679207 Substrate-driven gene expression in Roseburia inulinivorans: importance of inducible enzymes in the utilization of inulin and starch. Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1(Suppl 1):4672-9. doi: 10.1073/pnas.1000091107. Epub 2010 Aug 2. |
2011 Mar 15 | degradation | 6 | 1 | GH32 |
| PUL0425 | microarray | fructan, inulin | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 11 | 4 | GH32, GH91, GH91, GH32 |
| PUL0568 | clone and expression, enzyme activity assay, Northern Blot | sucrose | Clostridium beijerinckii | 10411273 The genes controlling sucrose utilization in Clostridium beijerinckii NCIMB 8052 constitute an operon. Microbiology (Reading). 1999 Jun;145 ( Pt 6):1461-1472. doi: 10.1099/13500872-145-6-1461. |
1999 Jun | degradation | 4 | 1 | GH32 |
| PUL0569 | clone and expression, enzyme activity assay, Northern Blot | levan | Bacillus subtilis | 11739774 yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis. Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413. |
2001 Dec | degradation | 3 | 1 | GH68, GH32 |
| PUL0581 | SDS-PAGE, enzyme activity assay | inulin | Microbulbifer sp. JAM-3301 | 22286980 Cloning and sequencing of inulinase and beta-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes. Appl Environ Microbiol. 2012 Apr;78(7):2493-5. doi: 10.1128/AEM.07442-11. Epub 2012 Jan 27. |
2012 Apr | degradation | 3 | 2 | GH32, CBM38, GH32 |
| PUL0587 | RT-PCR, enzyme activity assay | levan, inulin, fructan | Prevotella intermedia | 23266804 Identification and functional analysis of the gene cluster for fructan utilization in Prevotella intermedia. Gene. 2013 Feb 25;515(2):291-7. doi: 10.1016/j.gene.2012.12.023. Epub 2012 Dec 22. |
2013 Feb 25 | degradation | 3 | 1 | GH32 |
| PUL0604 | microarray, qRT-PCR, culureing methods | galactomannooligosaccharide | Lactobacillus plantarum WCFS1 | 31703861 Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14. |
2020 Apr | degradation | 6 | 2 | GH32, GH13_31, GH13 |
| PUL0632 | recombinant protein expression | sucrose, kestose, nystose, inulin | Roseburia inulinivorans DSM 16841 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | CBM66, GH32 |
| PUL0633 | recombinant protein expression | sucrose, kestose, nystose | Roseburia faecis M72 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0634 | recombinant protein expression | sucrose, kestose, nystose | Eubacterium rectale ATCC 33656 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 6 | 1 | GH32 |
| PUL0635 | recombinant protein expression | sucrose, kestose, nystose, fructooligosaccharide | Coprococcus eutactus JCM 31265 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 7 | 1 | CBM38, GH32, CBM66 |
| PUL0636 | recombinant protein expression | sucrose, kestose, nystose, fructooligosaccharide | Coprococcus eutactus JCM 31265 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 8 | 1 | GH32, CBM66 |
| PUL0637 | recombinant protein expression | sucrose, kestose, nystose | Faecalibacterium prausnitzii A2165 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0638 | recombinant protein expression | sucrose, kestose | Anaerostipes hadrus DSM 3319 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0639 | recombinant protein expression | sucrose, kestose | Anaerostipes hadrus DSM 3319 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 4 | 2 | GH32, GH32 |
| PUL0640 | recombinant protein expression | sucrose, kestose, nystose | Anaerostipes hadrus DSM 3319 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 10 | 1 | CBM66, CBM38, GH32 |
| PUL0641 | recombinant protein expression | sucrose, kestose | Anaerostipes caccae L1-92 DSM 14662 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 10 | 1 | GH32 |
| PUL0642 | recombinant protein expression | sucrose, kestose, nystose | Roseburia intestinalis L1-82 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 11 | 6 | GH36, GH13_31, GH13, CBM38, GH32, GH13_18, GH10, CBM86, CBM9, CBM0, CBM22, GH53, CBM61 |
Copyright 2020 © YIN LAB, UNL. All rights reserved. Designed by Catie Ausland and Jinfang Zheng. Maintained by Yanbin Yin.