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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0636

PubMed

33439065, Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.

Characterization method

recombinant protein expression

Genomic accession number

NZ_BLYL01000009.1

Nucelotide position range

14990-22272

Substrate

sucrose,kestose,nystose,fructooligosaccharide

Loci

JMF79_RS08410-JMF79_RS08440

Species

Coprococcus eutactus JCM 31265/33043

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- JMF79_RS08405 WP_055222460.1 0 - 4 (-) NZ_BLYL01000009.1:14990-14994 -
- JMF79_RS08410 WP_022217537.1 0 - 783 (-) NZ_BLYL01000009.1:14990-15773 -
- JMF79_RS08415 WP_055222459.1 1097 - 2555 (-) NZ_BLYL01000009.1:16087-17545 -
- JMF79_RS08420 WP_242859621.1 2577 - 2751 (-) NZ_BLYL01000009.1:17567-17741 -
- JMF79_RS08425 WP_004850770.1 2833 - 3508 (-) NZ_BLYL01000009.1:17823-18498 7.1.2.2
- JMF79_RS08430 WP_004850771.1 3787 - 5158 (-) NZ_BLYL01000009.1:18777-20148 -
- JMF79_RS08435 WP_022217533.1 5162 - 6926 (-) NZ_BLYL01000009.1:20152-21916 -
- JMF79_RS08440 WP_022217532.1 6962 - 7283 (-) NZ_BLYL01000009.1:21952-22273 7.1.2.2
- JMF79_RS08445 WP_015534272.1 7275 - 7283 (-) NZ_BLYL01000009.1:22265-22273 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 4 (-) CDS No
- 1 - 783 (-) CDS No
- 1098 - 2555 (-) CAZyme: GH32| GH32 Yes
- 2578 - 2751 (-) other Yes
- 2834 - 3508 (-) TC: gnl|TC-DB|P43435|3.A.2.2.2 Yes
- 3788 - 5158 (-) TC: gnl|TC-DB|Q08637|3.A.2.2.2 Yes
- 5163 - 6926 (-) TC: gnl|TC-DB|D3E1Z1|3.A.2.3.2 Yes
- 6963 - 7283 (-) CDS No
- 7276 - 7283 (-) CDS No

PUL ID

PUL0636

PubMed

33439065, Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.

Title

Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers.

Author

Tanno H, Fujii T, Hirano K, Maeno S, Tonozuka T, Sakamoto M, Ohkuma M, Tochio T, Endo A

Abstract

Butyrate produced by gut microbiota has multiple beneficial effects on host health, and oligosaccharides derived from host diets and glycans originating from host mucus are major sources of its production. A significant reduction of butyrate-producing bacteria has been reported in patients with inflammatory bowel diseases and colorectal cancers. Although gut butyrate levels are important for host health, oligosaccharide metabolic properties in butyrate producers are poorly characterized. We studied the metabolic properties of fructooligosaccharides (FOSs) and other prebiotic oligosaccharides (i.e. raffinose and xylooligosaccharides; XOSs) in gut butyrate producers. 1-Kestose (kestose) and nystose, FOSs with degrees of polymerization of 3 and 4, respectively, were also included. Fourteen species of butyrate producers were divided into four groups based on their oligosaccharide metabolic properties, which are group A (two species) metabolizing all oligosaccharides tested, group F (four species) metabolizing FOSs but not raffinose and XOSs, group XR (four species) metabolizing XOSs and/or raffinose but not FOSs, and group N (four species) metabolizing none of the oligosaccharides tested. Species assigned to groups A and XR are rich glycoside hydrolase (GH) holders, whereas those in groups F and N are the opposite. In total, 17 enzymes assigned to GH32 were observed in nine of the 14 butyrate producers tested, and species that metabolized FOSs had at least one active GH32 enzyme. The GH32 enzymes were divided into four clusters by phylogenetic analysis. Heterologous gene expression analysis revealed that the GH32 enzymes in each cluster had similar FOS degradation properties within clusters, which may be linked to the conservation/substitution of amino acids to bind with substrates in GH32 enzymes. This study provides important knowledge to understand the impact of FOS supplementation on the activation of gut butyrate producers. Abbreviations: SCFA, short chain fatty acid; FOS, fructooligosaccharide; XOS, xylooligosaccharide; CAZy, Carbohydrate Active Enzymes; CBM, carbohydrate-binding module; PUL, polysaccharide utilization locus; S6PH sucrose-6-phosphate hydrolase.