dbCAN-PUL

PUL ID	PUL0351
PubMed	1435727, Mol Gen Genet. 1992 Oct;235(1):22-32. doi: 10.1007/BF00286177.
Characterization method	enzyme activity assay
Genomic accession number	X81461.2
Nucelotide position range	3171-8055
Substrate	sucrose
Loci	cscRAKB
Species	Escherichia coli/562
Degradation or Biosynthesis	degradation

Cluster number	1
Gene name	Gene position	Gene type	Found by CGCFinder?
-	1 - 1248 (-)	TC: gnl\|TC-DB\|P30000\|2.A.1.5.3	Yes
-	1310 - 2233 (-)	other	Yes
-	2449 - 3882 (+)	CAZyme: GH32	Yes
-	3890 - 4885 (-)	TF: DBD-Pfam\|LacI,DBD-SUPERFAMILY\|0036955	No

PUL ID	PUL0351
PubMed	1435727, Mol Gen Genet. 1992 Oct;235(1):22-32. doi: 10.1007/BF00286177.
Title	Characterization of a chromosomally encoded, non-PTS metabolic pathway for sucrose utilization in Escherichia coli EC3132.
Author	Bockmann J, Heuel H, Lengeler JW
Abstract	A wild-type isolate, EC3132, of Escherichia coli, that is able to grow on sucrose was isolated and its csc genes (mnemonic for chromosomally coded sucrose genes) transferred to strains of E. coli K12. EC3132 and all sucrose-positive exconjugants and transductants invariably showed a D-serine deaminase (Dsd)-negative phenotype. The csc locus maps adjacent to dsdA, the structural gene for the D-serine deaminase, and contains an inducible regulon, controlled by a sucrose-specific repressor CscR, together with structural genes for a sucrose hydrolase (invertase) CscA, for a D-fructokinase CscK, and for a transport system CscB. Based on DNA sequencing studies, this last codes for a hydrophobic protein of 415 amino acids. CscB is closely related to the beta-galactoside transport system LacY (31.2% identical residues) and a raffinose transport system RafB (32.3% identical residues) of the enteric bacteria, both of the proton symport type. A two-dimensional model common to the three transport proteins, which is based on the integrated consensus sequence, will be discussed.