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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0635

PubMed

33439065, Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.

Characterization method

recombinant protein expression

Genomic accession number

NZ_BLYL01000002.1

Nucelotide position range

70950-76292

Substrate

sucrose,kestose,nystose,fructooligosaccharide

Loci

JMF79_RS02630-JMF79_RS02655

Species

Coprococcus eutactus JCM 31265/33043

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- JMF79_RS02630 WP_055222898.1 0 - 210 (-) NZ_BLYL01000002.1:70950-71160 -
- JMF79_RS02635 WP_242859629.1 260 - 1073 (-) NZ_BLYL01000002.1:71210-72023 -
- JMF79_RS02640 WP_082431264.1 1334 - 2864 (-) NZ_BLYL01000002.1:72284-73814 -
- JMF79_RS02645 WP_021388559.1 3190 - 4384 (-) NZ_BLYL01000002.1:74140-75334 -
- JMF79_RS02650 WP_009905235.1 4463 - 4667 (-) NZ_BLYL01000002.1:75413-75617 -
- JMF79_RS02655 WP_055222897.1 5085 - 5343 (-) NZ_BLYL01000002.1:76035-76293 -
- JMF79_RS02660 WP_055222895.1 5323 - 5343 (-) NZ_BLYL01000002.1:76273-76293 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 210 (-) TF: DBD-Pfam|HTH_3,DBD-SUPERFAMILY|0043143 No
- 261 - 1073 (-) CDS No
- 1335 - 2864 (-) CAZyme: GH32| GH32 No
- 3191 - 4384 (-) CDS No
- 4464 - 4667 (-) CDS No
- 5086 - 5343 (-) CDS No
- 5324 - 5343 (-) TF: DBD-Pfam|GerE,DBD-SUPERFAMILY|0039939 No

PUL ID

PUL0635

PubMed

33439065, Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.

Title

Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers.

Author

Tanno H, Fujii T, Hirano K, Maeno S, Tonozuka T, Sakamoto M, Ohkuma M, Tochio T, Endo A

Abstract

Butyrate produced by gut microbiota has multiple beneficial effects on host health, and oligosaccharides derived from host diets and glycans originating from host mucus are major sources of its production. A significant reduction of butyrate-producing bacteria has been reported in patients with inflammatory bowel diseases and colorectal cancers. Although gut butyrate levels are important for host health, oligosaccharide metabolic properties in butyrate producers are poorly characterized. We studied the metabolic properties of fructooligosaccharides (FOSs) and other prebiotic oligosaccharides (i.e. raffinose and xylooligosaccharides; XOSs) in gut butyrate producers. 1-Kestose (kestose) and nystose, FOSs with degrees of polymerization of 3 and 4, respectively, were also included. Fourteen species of butyrate producers were divided into four groups based on their oligosaccharide metabolic properties, which are group A (two species) metabolizing all oligosaccharides tested, group F (four species) metabolizing FOSs but not raffinose and XOSs, group XR (four species) metabolizing XOSs and/or raffinose but not FOSs, and group N (four species) metabolizing none of the oligosaccharides tested. Species assigned to groups A and XR are rich glycoside hydrolase (GH) holders, whereas those in groups F and N are the opposite. In total, 17 enzymes assigned to GH32 were observed in nine of the 14 butyrate producers tested, and species that metabolized FOSs had at least one active GH32 enzyme. The GH32 enzymes were divided into four clusters by phylogenetic analysis. Heterologous gene expression analysis revealed that the GH32 enzymes in each cluster had similar FOS degradation properties within clusters, which may be linked to the conservation/substitution of amino acids to bind with substrates in GH32 enzymes. This study provides important knowledge to understand the impact of FOS supplementation on the activation of gut butyrate producers. Abbreviations: SCFA, short chain fatty acid; FOS, fructooligosaccharide; XOS, xylooligosaccharide; CAZy, Carbohydrate Active Enzymes; CBM, carbohydrate-binding module; PUL, polysaccharide utilization locus; S6PH sucrose-6-phosphate hydrolase.