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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0031

PubMed

16523284, Appl Microbiol Biotechnol. 2006 Oct;72(5):975-81. doi: 10.1007/s00253-006-0358-x. Epub 2006 Mar 8.

Characterization method

RNA-Seq

Genomic accession number

NC_004307.2

Nucelotide position range

126816-130943

Substrate

sucrose

Loci

BLO107-cscB-cscA

Species

Bifidobacterium longum/216816

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BL_RS00545 WP_008782652.1 0 - 1525 (-) NC_004307.2:126816-128341 -
- BL_RS00550 WP_011067958.1 1535 - 2873 (-) NC_004307.2:128351-129689 -
- BL_RS00555 WP_011067959.1 3087 - 4128 (-) NC_004307.2:129903-130944 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1525 (-) CAZyme: GH32 Yes
- 1536 - 2873 (-) TC: gnl|TC-DB|P30000|2.A.1.5.3 Yes
- 3088 - 4128 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No

PUL ID

PUL0031

PubMed

16523284, Appl Microbiol Biotechnol. 2006 Oct;72(5):975-81. doi: 10.1007/s00253-006-0358-x. Epub 2006 Mar 8.

Title

A functional analysis of the Bifidobacterium longum cscA and scrP genes in sucrose utilization.

Author

Kullin B, Abratt VR, Reid SJ

Abstract

The role of genes involved in sucrose catabolism was investigated with a view to designing effective prebiotic substrates to encourage the growth of Bifidobacterium in the gut. Two gene clusters coding for sucrose utilisation in Bifidobacterium longum NCC2705 were identified in the published genome. The genes encoding putative sucrose degrading enzymes, namely, the scrP (sucrose phosphorylase) and the cscA (beta-fructofuranosidase), were cloned from B. longum NCIMB 702259(T) and expressed in Escherichia coli DH5alpha. Both complemented the sucrase negative phenotype of untransformed cells and showed specific sucrase activity. Transcriptional analysis of the expression of the genes in B. longum grown in the presence of various carbohydrate substrates showed induction of scrP gene expression in the presence of sucrose and raffinose, but not in the presence of glucose. The cscA gene showed no increased transcription in B. longum grown in the presence of any of the carbohydrates tested. Phylogenetic analysis indicates that the B. longum CscA protein belongs to a distinct phylogenetic cluster of intracellular fructosidases, which specifically cleave the shorter fructose oligosaccharides.