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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0587

PubMed

23266804, Gene. 2013 Feb 25;515(2):291-7. doi: 10.1016/j.gene.2012.12.023. Epub 2012 Dec 22.

Characterization method

RT-PCR,enzyme activity assay

Genomic accession number

CP019300.1

Nucelotide position range

699372-703335

Substrate

levan,inulin,fructan

Loci

fruABK

Species

Prevotella intermedia/28131

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BWX39_02890 APW31679.1 0 - 882 (-) CP019300.1:699372-700254 -
- BWX39_02895 APW31680.1 893 - 2048 (-) CP019300.1:700265-701420 -
- BWX39_02900 APW31681.1 2065 - 3964 (-) CP019300.1:701437-703336 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 882 (-) STP: STP|PfkB No
- 894 - 2048 (-) TC: gnl|TC-DB|Q8A6W8|2.A.1.7.17 Yes
- 2066 - 3964 (-) CAZyme: GH32 Yes

PUL ID

PUL0587

PubMed

23266804, Gene. 2013 Feb 25;515(2):291-7. doi: 10.1016/j.gene.2012.12.023. Epub 2012 Dec 22.

Title

Identification and functional analysis of the gene cluster for fructan utilization in Prevotella intermedia.

Author

Fuse H, Fukamachi H, Inoue M, Igarashi T

Abstract

Fructanase enzymes hydrolyze the beta-2,6 and beta-2,1 linkages of levan and inulin fructans, respectively. We analyzed the influence of fructan on the growth of Prevotella intermedia. The growth of P. intermedia was enhanced by addition of inulin, implying that P. intermedia could also use inulin. Based on this finding, we identified and analyzed the genes encoding a putative fructanase (FruA), sugar transporter (FruB), and fructokinase (FruK) in the genome of strain ATCC25611. Transcript analysis by RT-PCR showed that the fruABK genes were co-transcribed as a single mRNA and semi-quantitative analysis confirmed that the fruA gene was induced in response to fructose and inulin. Recombinant FruA and FruK were purified and characterized biochemically. FruA strongly hydrolyzed inulin, with slight degradation of levan via an exo-type mechanism, revealing that FruA is an exo-beta-d-fructanase. FruK converted fructose to fructose-6-phosphate in the presence of ATP, confirming that FruK is an ATP-dependent fructokinase. These results suggest that P. intermedia can utilize fructan as a carbon source for growth, and that the fructanase, sugar transporter, and fructokinase proteins we identified are involved in this fructan utilization.