Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0005 enzyme activity assay, crystallization beta-glucan, sophorose, laminaribiose Listeria innocua 26886583
Functional and Structural Analysis of a beta-Glucosidase Involved in beta-1,2-Glucan Metabolism in Listeria innocua. PLoS One. 2016 Feb 17;11(2):e0148870. doi: 10.1371/journal.pone.0148870. eCollection 2016.
2016 degradation 2 1 GH94, GH3
PUL0049 fosmid library screen beta-glucan, galactomannan feces metagenome 29601586
Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018.
2018 degradation 29 6 GH43_17, GH26, GH16
PUL0118 qRT-PCR, affinity gel electrophoresis, isothermal titration calorimetry laminarin, beta-glucan Bacteroides uniformis 32265336
Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides. mBio. 2020 Apr 7;11(2). pii: mBio.00095-20. doi: 10.1128/mBio.00095-20.
2020 Apr 7 degradation 7 3 CBM6, GH3, GH158, GH16
PUL0125 affinity gel electrophoresis, carbohydrate binding assay, RNA-seq beta-glucan Bacteroides ovatus 31062073, 32801182
Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Mol Life Sci. 2019 Nov;76(21):4319-4340. doi: 10.1007/s00018-019-03115-3. Epub 2019 May 6. Appl Environ Microbiol. 2020 Oct 1;86(20). pii: AEM.01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1.
2019 Nov,2020 Oct 1 degradation 6 2 GH16, GH3
PUL0170 enzyme activity assay beta-glucan Bacteroides ovatus 29141231
Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Cell Rep. 2017 Nov 14;21(7):2030. doi: 10.1016/j.celrep.2017.11.013.
2017 Nov 14 degradation 7 3 GH3, GH16, GH3
PUL0187 qRT-PCR, enzyme activity assay beta-glucan Paenibacillus sp. JDR-2 26746717
A 1,3-1,4-beta-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2. Appl Environ Microbiol. 2016 Jan 8;82(6):1789-1798. doi: 10.1128/AEM.03526-15.
2016 Jan 8 degradation 7 2 CBM4, CBM54, CBM6, GH16, GH16
PUL0308 microarray, enzyme activity assay, high performance anion exchange chromatography, mass spectrometry, RNA-seq beta-glucan Bacteroides ovatus 29020628, 22205877, 32801182
Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Rep. 2017 Oct 10;21(2):417-430. doi: 10.1016/j.celrep.2017.09.049. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Appl Environ Microbiol. 2020 Oct 1;86(20). pii: AEM.01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1.
2017 Oct 10,2011 Dec,2020 Oct 1 degradation 5 3 GH16, GH3
PUL0325 RT-PCR, enzyme activity assay beta-glucan Bacteroides thetaiotaomicron 28461332
A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase. J Biol Chem. 2017 Jun 23;292(25):10639-10650. doi: 10.1074/jbc.M117.787606. Epub 2017 May 1.
2017 Jun 23 degradation 6 2 GH30, GH30_3, GH3
PUL0326 gene deletion mutant and growth assay, enzyme activity assay, thin layer chromatography beta-glucan Bacteroides ovatus 28461332
A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase. J Biol Chem. 2017 Jun 23;292(25):10639-10650. doi: 10.1074/jbc.M117.787606. Epub 2017 May 1.
2017 Jun 23 degradation 13 2 GH73
PUL0332 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 19 9 GH5, GH5_2, GH5_7, GH5, CE7, GH2, GH94, GH97, GH127, GH127
PUL0333 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 22 3 GH9, GH31, GH9
PUL0334 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 23 5 GH9, GH31, GH9, GH9, CE4
PUL0335 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 31 4 GH13, GH97, GH3, GH158, GH16
PUL0336 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 25 3 GH3, GH158, GH16
PUL0337 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 29 3 GH16, GH158, GH3
PUL0338 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 34 4 GH16, GH158, GH3, GH97
PUL0339 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 24 4 GH97, GH3, GH16, GH20
PUL0340 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 37 1 GH5_2, GH5
PUL0341 fosmid library screen, enzyme activity assay, thin layer chromatography carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 43 3 GH32, GH91, GH5, GH5_2
PUL0343 gene deletion mutant and growth assay, enzyme activity assay, Western Blot, isothermal titration calorimetry carboxymethylcellulose, xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 39 1 GH5, GH5_2
PUL0406 high performance anion exchange chromatography beta-glucan Coprothermobacter proteolyticus 30315317
From proteins to polysaccharides: lifestyle and genetic evolution of Coprothermobacter proteolyticus. ISME J. 2019 Mar;13(3):603-617. doi: 10.1038/s41396-018-0290-y. Epub 2018 Oct 12.
2019 Mar degradation 20 3 GH16, GH3, GH18
PUL0474 growth assay beta-glucan, xylan Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 9 5 GH30_1, GH30, GH30_1, GH30, GH3, GH30_3, GH3
PUL0477 growth assay beta-glucan Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 6 2 GH16, GH16
PUL0526 microarray, qPCR beta-glucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 6 2 GH30_3, GH30, GH3
PUL0528 microarray, qPCR beta-glucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 7 3 GH3, GH16, GH3
PUL0537 RNA-Seq beta-glucan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 7 3 GH3, GH3, GH157
PUL0543 RNA-Seq cellobiose, beta-glucan, glucosamine Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 6 3 GH30, GH30_3, GH2, CBM57, GH88
PUL0551 RNA-Seq cellobiose, beta-glucan, glucomannan, laminarin Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 6 2 GH16, GH3