PUL ID

PUL0338

PubMed

28091525, Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.

Characterization method

fosmid library screen, enzyme activity assay, thin layer chromatography

Genomic accession number

LT674128

Nucelotide position range

328-43016

Substrate

xylan,beta-glucan,lichenan

Loci

SIP56396.1-SIP56429.1

Species

uncultured bacterium/77133

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - SIP56396.1 328 - 1504 (-) LT674128.1:656-1832 -
- - SIP56397.1 1911 - 3612 (+) LT674128.1:2239-3940 2.7.13.3
- - SIP56398.1 3776 - 5171 (-) LT674128.1:4104-5499 3.6.-.-
- - SIP56399.1 5450 - 6329 (-) LT674128.1:5778-6657 2.4.2.1
- - SIP56400.1 6423 - 7425 (-) LT674128.1:6751-7753 -
- - SIP56401.1 7476 - 7947 (-) LT674128.1:7804-8275 -
- - SIP56402.1 7955 - 9386 (-) LT674128.1:8283-9714 -
- - SIP56403.1 9552 - 10302 (+) LT674128.1:9880-10630 -
- - SIP56404.1 10323 - 10692 (+) LT674128.1:10651-11020 -
- - SIP56405.1 10713 - 11730 (-) LT674128.1:11041-12058 -
- - SIP56406.1 11756 - 12335 (-) LT674128.1:12084-12663 -
- - SIP56407.1 12342 - 13125 (-) LT674128.1:12670-13453 -
- - SIP56408.1 13078 - 13564 (-) LT674128.1:13406-13892 -
- - SIP56409.1 13566 - 14520 (-) LT674128.1:13894-14848 2.4.2.53
- - SIP56410.1 14575 - 15121 (-) LT674128.1:14903-15449 -
- - SIP56411.1 15816 - 18768 (+) LT674128.1:16144-19096 -
- - SIP56412.1 19058 - 22211 (+) LT674128.1:19386-22539 -
- - SIP56413.1 22229 - 23825 (+) LT674128.1:22557-24153 -
- - SIP56414.1 23898 - 24108 (+) LT674128.1:24226-24436 -
- - SIP56415.1 24193 - 25138 (+) LT674128.1:24521-25466 3.2.1.73
- - SIP56416.1 25227 - 26520 (+) LT674128.1:25555-26848 -
- - SIP56417.1 26531 - 28775 (+) LT674128.1:26859-29103 3.2.1.21
- - SIP56418.1 28878 - 30168 (-) LT674128.1:29206-30496 4.2.1.11
- - SIP56419.1 30232 - 30901 (-) LT674128.1:30560-31229 -
- - SIP56420.1 30914 - 31292 (-) LT674128.1:31242-31620 -
- - SIP56421.1 32017 - 32503 (+) LT674128.1:32345-32831 -
- - SIP56422.1 32730 - 34884 (+) LT674128.1:33058-35212 3.2.1.22
- - SIP56423.1 35089 - 36031 (+) LT674128.1:35417-36359 -
- - SIP56424.1 36034 - 36841 (-) LT674128.1:36362-37169 -
- - SIP56425.1 37012 - 38425 (+) LT674128.1:37340-38753 2.4.2.14
- - SIP56426.1 38621 - 39845 (+) LT674128.1:38949-40173 3.4.11.4
- - SIP56427.1 39876 - 40965 (+) LT674128.1:40204-41293 2.1.2.10
- - SIP56428.1 41056 - 42583 (-) LT674128.1:41384-42911 -
- - SIP56429.1 42764 - 43016 (-) LT674128.1:43092-43344 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 329 - 1504 (-) TC: gnl|TC-DB|P76628|2.A.1.36.3 Yes
- 1912 - 3612 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 Yes
- 3777 - 5171 (-) other Yes
- 5451 - 6329 (-) other Yes
- 6424 - 7425 (-) other Yes
- 7477 - 7947 (-) other Yes
- 7956 - 9386 (-) STP: STP|TPR_2 Yes
- 9553 - 10302 (+) other Yes
- 10324 - 10692 (+) TC: gnl|TC-DB|Q64X46|2.A.7.34.3 Yes
- 10714 - 11730 (-) other Yes
- 11757 - 12335 (-) TC: gnl|TC-DB|O27840|2.A.107.1.2 Yes
- 12343 - 13125 (-) other Yes
- 13079 - 13564 (-) other Yes
- 13567 - 14520 (-) TC: gnl|TC-DB|P77757|4.D.2.1.8 Yes
- 14576 - 15121 (-) other Yes
- 15817 - 18768 (+) TF: DBD-Pfam|GerE Yes
- 19059 - 22211 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 22230 - 23825 (+) other Yes
- 23899 - 24108 (+) other Yes
- 24194 - 25138 (+) CAZyme: GH16 Yes
- 25228 - 26520 (+) CAZyme: GH158 Yes
- 26532 - 28775 (+) CAZyme: GH3 Yes
- 28879 - 30168 (-) other Yes
- 30233 - 30901 (-) other Yes
- 30915 - 31292 (-) TC: gnl|TC-DB|P37002|1.A.43.1.1 Yes
- 32018 - 32503 (+) other Yes
- 32731 - 34884 (+) CAZyme: GH97 Yes
- 35090 - 36031 (+) STP: STP|Fer4 No
- 36035 - 36841 (-) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 No
- 37013 - 38425 (+) STP: STP|Pribosyltran No
- 38622 - 39845 (+) CDS No
- 39877 - 40965 (+) CDS No
- 41057 - 42583 (-) CDS No
- 42765 - 43016 (-) CDS No

PUL ID

PUL0338

PubMed

28091525, Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.

Title

A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic.

Author

Patrascu O, Beguet-Crespel F, Marinelli L, Le Chatelier E, Abraham AL, Leclerc M, Klopp C, Terrapon N, Henrissat B, Blottiere HM, Dore J, Bera-Maillet C

Abstract

The digestion of dietary fibers is a major function of the human intestinal microbiota. So far this function has been attributed to the microorganisms inhabiting the colon, and many studies have focused on this distal part of the gastrointestinal tract using easily accessible fecal material. However, microbial fermentations, supported by the presence of short-chain fatty acids, are suspected to occur in the upper small intestine, particularly in the ileum. Using a fosmid library from the human ileal mucosa, we screened 20,000 clones for their activities against carboxymethylcellulose and xylans chosen as models of the major plant cell wall (PCW) polysaccharides from dietary fibres. Eleven positive clones revealed a broad range of CAZyme encoding genes from Bacteroides and Clostridiales species, as well as Polysaccharide Utilization Loci (PULs). The functional glycoside hydrolase genes were identified, and oligosaccharide break-down products examined from different polysaccharides including mixed-linkage beta-glucans. CAZymes and PULs were also examined for their prevalence in human gut microbiome. Several clusters of genes of low prevalence in fecal microbiome suggested they belong to unidentified strains rather specifically established upstream the colon, in the ileum. Thus, the ileal mucosa-associated microbiota encompasses the enzymatic potential for PCW polysaccharide degradation in the small intestine.