| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0120 | recombinant protein expression, RNA-Seq, differential gene expression | N-glycan, mucin | Bacteroides thetaiotaomicron | 31160824 Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. |
2019 Sep | degradation | 6 | 2 | CBM14, GH18 |
| PUL0144 | enzyme activity assay, Western Blot | chitin | Thermococcus kodakarensis | 16199574 Characterization of a novel glucosamine-6-phosphate deaminase from a hyperthermophilic archaeon. J Bacteriol. 2005 Oct;187(20):7038-44. doi: 10.1128/JB.187.20.7038-7044.2005. |
2005 Oct | degradation | 12 | 3 | GH35, GH1, CBM2, GH18 |
| PUL0171 | qRT-PCR, RNA-Seq | N-glycan | Bacteroides fragilis | 27353652 cis-Encoded Small RNAs, a Conserved Mechanism for Repression of Polysaccharide Utilization in Bacteroides. J Bacteriol. 2016 Aug 25;198(18):2410-8. doi: 10.1128/JB.00381-16. Print 2016 Sep 15. |
2016 Sep 15 | degradation | 7 | 1 | GH18 |
| PUL0208 | growth assay | chitin | Pseudoalteromonas luteoviolacea | 31213521 Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential. mSystems. 2019 Jun 18;4(4):e00060-19. doi: 10.1128/mSystems.00060-19. |
2019 Jun 18 | degradation | 3 | 3 | CBM5, GH18, CBM5, AA10, CBM5, GH18 |
| PUL0211 | enzyme activity assay, gene deletion mutant and growth assay, thin layer chromatography | N-glycan | Xanthomonas campestris pv. campestris | 25586188, 25205095 The N-Glycan cluster from Xanthomonas campestris pv. campestris: a toolbox for sequential plant N-glycan processing. The plant pathogen Xanthomonas campestris pv. campestris exploits N-acetylglucosamine during infection. J Biol Chem. 2015 Mar 6;290(10):6022-36. doi: 10.1074/jbc.M114.624593. Epub 2015 Jan 13. mBio. 2014 Sep 9;5(5):e01527-14. doi: 10.1128/mBio.01527-14. |
2015 Mar 6,2014 Sep 9 | degradation | 9 | 6 | GH29, GH18, GH20, GH2, GH3, GH125, GH92, GH35 |
| PUL0269 | RT-PCR | chitin | Haloferax mediterranei | 23674154 Characterization of genes for chitin catabolism in Haloferax mediterranei. Appl Microbiol Biotechnol. 2014 Feb;98(3):1185-94. doi: 10.1007/s00253-013-4969-8. Epub 2013 May 15. |
2014 Feb | degradation | 16 | 4 | GH3, CBM5, CBM5, GH18, CBM5, GH18 |
| PUL0307 | enzyme activity assay | chitin | Serratia marcescens subsp. marcescens | 29229757, 8757722 Structure and activity of ChiX: a peptidoglycan hydrolase required for chitinase secretion by Serratia marcescens. Comparative studies of chitinases A and B from Serratia marcescens. Biochem J. 2018 Jan 23;475(2):415-428. doi: 10.1042/BCJ20170633. Microbiology (Reading). 1996 Jul;142 ( Pt 7):1581-9. doi: 10.1099/13500872-142-7-1581. |
2018 Jan 23,1996 Jul | degradation | 6 | 2 | CBM5, GH18, AA10 |
| PUL0344 | gene deletion mutant and growth assay, protein structure characterization | chitin | Flavobacterium johnsoniae | 27933102, 32792608 A polysaccharide utilization locus from Flavobacterium johnsoniae enables conversion of recalcitrant chitin. Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae. Biotechnol Biofuels. 2016 Nov 28;9:260. doi: 10.1186/s13068-016-0674-z. eCollection 2016. Sci Rep. 2020 Aug 13;10(1):13775. doi: 10.1038/s41598-020-70749-w. |
2016,2020 Aug 13 | degradation | 11 | 3 | GH18, GH20, GH18 |
| PUL0355 | microarray | lactose, host glycan | Bacteroides thetaiotaomicron | 16968696 Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12. |
2006 Nov 24 | degradation | 8 | 1 | GH30_4, GH30, GH18 |
| PUL0356 | microarray | lactose, host glycan | Bacteroides thetaiotaomicron | 16968696 Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12. |
2006 Nov 24 | degradation | 9 | 1 | GH18 |
| PUL0376 | microarray, qPCR | mucin, O-glycan | Bacteroides thetaiotaomicron | 18996345 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2008 Nov 13 | degradation | 9 | 2 | GH16, GH18 |
| PUL0397 | gene deletion mutant and growth assay | N-acetylglucosamine | Capnocytophaga canimorsus | 21762219 The genome and surface proteome of Capnocytophaga canimorsus reveal a key role of glycan foraging systems in host glycoproteins deglycosylation. Mol Microbiol. 2011 Aug;81(4):1050-60. doi: 10.1111/j.1365-2958.2011.07750.x. Epub 2011 Jul 18. |
2011 Aug | degradation | 5 | 1 | GH18 |
| PUL0406 | high performance anion exchange chromatography | beta-glucan | Coprothermobacter proteolyticus | 30315317 From proteins to polysaccharides: lifestyle and genetic evolution of Coprothermobacter proteolyticus. ISME J. 2019 Mar;13(3):603-617. doi: 10.1038/s41396-018-0290-y. Epub 2018 Oct 12. |
2019 Mar | degradation | 20 | 3 | GH16, GH3, GH18 |
| PUL0422 | microarray | host glycan | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 4 | 1 | GH18 |
| PUL0424 | microarray | host glycan | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 18 | 1 | GH18, GH163, GH130, GH92 |
| PUL0442 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 9 | 3 | CBM5, GH18, GH18, GH64, CBM6, GH16, CBM6 |
| PUL0465 | microarray, qPCR | mucin | Bacteroides thetaiotaomicron | 18996345 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2008 Nov 13 | degradation | 8 | 2 | GH18 |
| PUL0467 | microarray, qPCR | host glycan | Bacteroides thetaiotaomicron | 18996345 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2008 Nov 13 | degradation | 14 | 5 | GH29, CBM32, GH2, GH20, GH18 |
| PUL0470 | microarray, qPCR | mucin | Bacteroides thetaiotaomicron | 18996345 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2008 Nov 13 | degradation | 7 | 1 | GH18 |
| PUL0475 | clone and expression, gene deletion mutant and growth assay | cellobiose, cellotriose | Streptomyces reticuli | 10347054 Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli. Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999. |
1999 Jun | degradation | 7 | 1 | GH18, CBM2 |
| PUL0487 | growth assay | chitin | Flavobacterium johnsoniae | 19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 10 | 3 | GH18, GH20, GH18 |
| PUL0497 | clone and expression, enzyme activity assay | chitin | Pseudoalteromonas piscicida | 11772635 Identification and characterization of the gene cluster involved in chitin degradation in a marine bacterium, Alteromonas sp. strain O-7. Appl Environ Microbiol. 2002 Jan;68(1):263-70. doi: 10.1128/AEM.68.1.263-270.2002. |
2002 Jan | degradation | 3 | 2 | CBM5, GH18, CBM5, AA10, CBM5, GH18 |
| PUL0556 | gene deletion mutant and growth assay, qRT-PCR, microarray, enzyme activity assay | N-glycan | Bacteroides fragilis | 25139987 Efficient utilization of complex N-linked glycans is a selective advantage for Bacteroides fragilis in extraintestinal infections. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19. |
2014 Sep 2 | degradation | 7 | 1 | GH18 |
| PUL0567 | clone and expression, enzyme activity assay | chitin | Pseudoalteromonas sp. S9 | 10220172 Multiple genes involved in chitin degradation from the marine bacterium Pseudoalteromonas sp. strain S91. Microbiology (Reading). 1999 Apr;145 ( Pt 4):925-934. doi: 10.1099/13500872-145-4-925. |
1999 Apr | degradation | 3 | 3 | CBM5, GH18, AA10, CBM5, CBM5, GH18 |
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