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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0397

PubMed

21762219, Mol Microbiol. 2011 Aug;81(4):1050-60. doi: 10.1111/j.1365-2958.2011.07750.x. Epub 2011 Jul 18.

Characterization method

gene deletion mutant and growth assay

Genomic accession number

CP002113.1

Nucelotide position range

931942-940427

Substrate

N-acetylglucosamine

Loci

Ccan_08700–Ccan_08740

Species

Capnocytophaga canimorsus/28188

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- Ccan_08700 AEK22988.1 0 - 3123 (+) CP002113.1:931942-935065 -
- Ccan_08710 AEK22989.1 3145 - 4783 (+) CP002113.1:935087-936725 -
- Ccan_08720 AEK22990.1 4803 - 6141 (+) CP002113.1:936745-938083 -
- Ccan_08730 AEK22991.1 6147 - 7473 (+) CP002113.1:938089-939415 -
- Ccan_08740 AEK22992.1 7484 - 8486 (+) CP002113.1:939426-940428 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 3123 (+) TC: gnl|TC-DB|Q8A8X1|1.B.14.6.13 Yes
- 3146 - 4783 (+) TC: gnl|TC-DB|Q8A0N7|8.A.46.2.2 Yes
- 4804 - 6141 (+) CAZyme: GH18 Yes
- 6148 - 7473 (+) CDS No
- 7485 - 8486 (+) CDS No

PUL ID

PUL0397

PubMed

21762219, Mol Microbiol. 2011 Aug;81(4):1050-60. doi: 10.1111/j.1365-2958.2011.07750.x. Epub 2011 Jul 18.

Title

The genome and surface proteome of Capnocytophaga canimorsus reveal a key role of glycan foraging systems in host glycoproteins deglycosylation.

Author

Manfredi P, Renzi F, Mally M, Sauteur L, Schmaler M, Moes S, Jeno P, Cornelis GR

Abstract

Capnocytophaga canimorsus are commensal Gram-negative bacteria from dog's mouth that cause rare but dramatic septicaemia in humans. C. canimorsus have the unusual property to feed on cultured mammalian cells, including phagocytes, by harvesting the glycan moiety of cellular glycoproteins. To understand the mechanism behind this unusual property, the genome of strain Cc5 was sequenced and analysed. In addition, Cc5 bacteria were cultivated onto HEK 293 cells and the surface proteome was determined. The genome was found to encode many lipoproteins encoded within 13 polysaccharide utilization loci (PULs) typical of the Flavobacteria-Bacteroides group. PULs encode surface exposed feeding complexes resembling the archetypal starch utilization system (Sus). The products of at least nine PULs were detected among the surface proteome and eight of them represented more than half of the total peptides detected from the surface proteome. Systematic deletions of the 13 PULs revealed that half of these Sus-like complexes contributed to growth on animal cells. The complex encoded by PUL5, one of the most abundant ones, was involved in foraging glycans from glycoproteins. It was essential for growth on cells and contributed to survival in mice. It thus represents a fitness factor during infection.