dbCAN-PUL

PUL ID	PUL0556
PubMed	25139987, Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19.
Characterization method	gene deletion mutant and growth assay,qRT-PCR,microarray,enzyme activity assay
Genomic accession number	NC_016776.1
Nucelotide position range	1583511-1593729
Substrate	N-glycan
Loci	BF638R_1323-BF638R_1330
Species	Bacteroides fragilis/817
Degradation or Biosynthesis	degradation

PUL ID	PUL0556
PubMed	25139987, Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19.
Title	Efficient utilization of complex N-linked glycans is a selective advantage for Bacteroides fragilis in extraintestinal infections.
Author	Cao Y, Rocha ER, Smith CJ
Abstract	Bacteroides fragilis is the most common anaerobe isolated from clinical infections, and in this report we demonstrate a characteristic of the species that is critical to their success as an opportunistic pathogen. Among the Bacteroides spp. in the gut, B. fragilis has the unique ability of efficiently harvesting complex N-linked glycans from the glycoproteins common to serum and serous fluid. This activity is mediated by an outer membrane protein complex designated as Don. Using the abundant serum glycoprotein transferrin as a model, it has been shown that B. fragilis alone can rapidly and efficiently deglycosylate this protein in vitro and that transferrin glycans can provide the sole source of carbon and energy for growth in defined media. We then showed that transferrin deglycosylation occurs in vivo when B. fragilis is propagated in the rat tissue cage model of extraintestinal growth, and that this ability provides a competitive advantage in vivo over strains lacking the don locus.