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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0475

PubMed

10347054, Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999.

Characterization method

clone and expression,gene deletion mutant and growth assay

Genomic accession number

LN997842.1

Nucelotide position range

3957983-3965681

Substrate

cellobiose,cellotriose

Loci

cebREFG-bglC

Species

Streptomyces reticuli/1926

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- TUE45_03411 CUW28691.1 0 - 4 (-) LN997842.1:3957983-3957987 -
degA_2 TUE45_03412 CUW28692.1 0 - 1056 (-) LN997842.1:3957983-3959039 -
lacE_1 TUE45_03413 CUW28693.1 1366 - 2698 (+) LN997842.1:3959349-3960681 -
lacF_8 TUE45_03414 CUW28694.1 2841 - 3720 (+) LN997842.1:3960824-3961703 -
lacG TUE45_03415 CUW28695.1 3716 - 4601 (+) LN997842.1:3961699-3962584 -
- TUE45_03416 CUW28696.1 4721 - 6152 (+) LN997842.1:3962704-3964135 3.2.1.21
chiA TUE45_03417 CUW28697.1 6223 - 7699 (-) LN997842.1:3964206-3965682 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 4 (-) CDS No
degA_2 1 - 1056 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No
lacE_1 1367 - 2698 (+) TC: gnl|TC-DB|Q9X9R7|3.A.1.1.23 Yes
lacF_8 2842 - 3720 (+) TC: gnl|TC-DB|Q9X9R6|3.A.1.1.23 Yes
lacG 3717 - 4601 (+) TC: gnl|TC-DB|Q9X9R5|3.A.1.1.23 Yes
- 4722 - 6152 (+) TC: gnl|TC-DB|Q9UEF7|8.A.49.1.1 Yes
chiA 6224 - 7699 (-) CAZyme: GH18|CBM2 Yes

PUL ID

PUL0475

PubMed

10347054, Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999.

Title

Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli.

Author

Schlosser A, Jantos J, Hackmann K, Schrempf H

Abstract

Streptomyces reticuli has an inducible ATP-dependent uptake system specific for cellobiose and cellotriose. By reversed genetics a gene cluster encoding components of a binding protein-dependent cellobiose and cellotriose ABC transporter was cloned and sequenced. The deduced gene products comprise a regulatory protein (CebR), a cellobiose binding lipoprotein (CebE), two integral membrane proteins (CebF and CebG), and the NH2-terminal part of an intracellular beta-glucosidase (BglC). The gene for the ATP binding protein MsiK is not linked to the ceb operon. We have shown earlier that MsiK is part of two different ABC transport systems, one for maltose and one for cellobiose and cellotriose, in S. reticuli and Streptomyces lividans. Transcription of polycistronic cebEFG and bglC mRNAs is induced by cellobiose, whereas the cebR gene is transcribed independently. Immunological experiments showed that CebE is synthesized during growth with cellobiose and that MsiK is produced in the presence of several sugars at high or moderate levels. The described ABC transporter is the first one of its kind and is the only specific cellobiose/cellotriose uptake system of S. reticuli, since insertional inactivation of the cebE gene prevents high-affinity uptake of cellobiose.