Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0001 RNA-Seq, substrate binding assay, enzyme activity assay, mass spectrometry beta-mannan Roseburia intestinalis 30796211
The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.
2019 Feb 22 degradation 15 7 GH1, CE2, GH130, GH130, GH36, GH113
PUL0030 isothermal calorimetric titration, gene deletion mutant and growth assay, enzyme activity assay mannan Bacillus sp. N16-5 26978267, 30351049
A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization. Galactomannan Degrading Enzymes from the Mannan Utilization Gene Cluster of Alkaliphilic Bacillus sp. N16-5 and Their Synergy on Galactomannan Degradation. PLoS One. 2016 Mar 15;11(3):e0150059. doi: 10.1371/journal.pone.0150059. eCollection 2016. J Agric Food Chem. 2018 Oct 24;66(42):11055-11063. doi: 10.1021/acs.jafc.8b03878. Epub 2018 Oct 15.
2016,2018 Oct 24 degradation 12 7 GH130, GH130, GH27, CE7
PUL0050 fosmid library screen cellulose, galactomannan feces metagenome 29601586
Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018.
2018 degradation 20 7 GH5_4, GH5, GH26, GH130, GH3, GH94
PUL0117 expression of recombinant proteins, RNA-Seq, differential gene expression N-glycan Bacteroides thetaiotaomicron 31160824
Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.
2019 Sep degradation 22 4 GH92, GH130, GH163, GH20
PUL0129 enzyme activity assay mannan gut metagenome 30356154
Interspecies cross-feeding orchestrates carbon degradation in the rumen ecosystem. Nat Microbiol. 2018 Nov;3(11):1274-1284. doi: 10.1038/s41564-018-0225-4. Epub 2018 Oct 24.
2018 Nov degradation 12 6 GH5, GH5_4, GH26, GH26, GH5, GH5_4, CE7, CE7, GH26, GH130
PUL0163 mass spectrometry, sequence homology analysis alpha-mannan Bacteroides thetaiotaomicron 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 21 7 GH92, GH38, CBM32, GT32, GT32, GH130, GH125, GH76, GH92, GH76
PUL0164 mass spectrometry, sequence homology analysis, differential gene expression beta-mannan Leeuwenhoekiella sp. MAR_2009_132 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 19 9 CE2, GH3, GH5_7, GH26, GH130, GH26, GH5_2, GH5, GH27, GH9, GH26
PUL0165 mass spectrometry, sequence homology analysis, differential gene expression beta-mannan Salegentibacter sp. Hel_I_6 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 17 6 GH9, GH27, GH5, GH5_2, GH26, GH130, GH26
PUL0167 mass spectrometry, sequence homology analysis beta-mannan Bacteroides ovatus 30246424
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 12 4 GH130, GH36, GH26, GH26
PUL0175 enzyme activity assay galactomannan Cellvibrio mixtus 16842369
Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus. FEMS Microbiol Lett. 2006 Aug;261(1):123-32. doi: 10.1111/j.1574-6968.2006.00342.x.
2006 Aug degradation 4 3 GH130, GH5_7, GH5, GH27
PUL0178 enzyme activity assay, enzyme specificity assay, substrate specificity assay galactomannan Bacteroides ovatus 27288925, 22205877
A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2016 Jul,2011 Dec degradation 14 4 GH130, GH36, GH26, GH26
PUL0179 enzyme activity assay, enzyme specificity assay, substrate specificity assay galactomannan Bacteroides ovatus 27288925
A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28.
2016 Jul degradation 15 3 CE7, GH130, GH26, GH26
PUL0185 isothermal calorimetric titration, gene deletion mutant and growth assay mannan Bacillus sp. N16-5 26978267
A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization. PLoS One. 2016 Mar 15;11(3):e0150059. doi: 10.1371/journal.pone.0150059. eCollection 2016.
2016 degradation 12 6 GH130, GH130, GH27, CE7
PUL0217 RNA-Seq galactomannan, glucomannan, glucose, mannose Caldanaerobius polysaccharolyticus 25342756
Structural and biochemical basis for mannan utilization by Caldanaerobius polysaccharolyticus strain ATCC BAA-17. J Biol Chem. 2014 Dec 12;289(50):34965-77. doi: 10.1074/jbc.M114.579904. Epub 2014 Oct 23.
2014 Dec 12 degradation 7 2 GH130, GH5_36
PUL0223 enzyme specificity assay, enzyme activity assay N-glycan Bacteroides thetaiotaomicron 23943617
Discovery of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans. J Biol Chem. 2013 Sep 20;288(38):27366-27374. doi: 10.1074/jbc.M113.469080. Epub 2013 Aug 13.
2013 Sep 20 degradation 9 4 GH92, GH130, GH163
PUL0239 fosmid library screen, sequence homology analysis cellulose Prevotella sp. Sc00026 24448980
Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22.
2014 Mar degradation 18 10 GH26, GH5_4, GH5_7, GH130, GH26, CE7, GH36, GH3
PUL0293 RT-qPCR, gene deletion mutant and growth assay mannan Bacillus sp. N16-5 29360976
Transcriptional regulation of the mannan utilization genes in the alkaliphilic Bacillus sp. N16-5. FEMS Microbiol Lett. 2018 Feb 1;365(4). pii: 4816728. doi: 10.1093/femsle/fnx280.
2018 Feb 1 degradation 12 7 GH130, GH130, GH27, CE7
PUL0378 microarray, qPCR alpha-mannan Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 20 5 GH92, CBM32, GH38, GT32, GT32, GH130, GH125, GH76, GH92, GH76
PUL0408 enzyme activity assay, thin layer chromatography mannan Bacteroides fragilis 24217874
The mannobiose-forming exo-mannanase involved in a new mannan catabolic pathway in Bacteroides fragilis. Arch Microbiol. 2014 Jan;196(1):17-23. doi: 10.1007/s00203-013-0938-y. Epub 2013 Nov 12.
2014 Jan degradation 4 2 GH26, GH130
PUL0419 microarray plant polysaccharide Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 11 5 GH5, GH5_2, GH5_5, GH2, GH5, GH5_5, GH140, GH130, CBM67, GH78
PUL0424 microarray host glycan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 18 1 GH18, GH163, GH130, GH92
PUL0441 mass spectrometry, high performance anion exchange chromatography glucomannan, galactomannan Chitinophaga pinensis 28069559
Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6.
2017 Mar 6 degradation 6 2 GH130, CBM13, GH43_26
PUL0472 microarray, qPCR mucin Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 28 8 GH38, GH92, CBM32, GH2, GH78, CBM67, CE1, GH92, GH92, GH130, GH43_8
PUL0488 growth assay beta-mannan Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 14 7 GH5, GH5_2, GH27, GH26, GH130, GH26, GH5_7, GH97
PUL0538 RNA-Seq galactomannan, glucomannan, cellobiose, xyloglucan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 15 7 GH130, GH26, GH26, GH5_2, GH5, GH5_7, CE7, GH3
PUL0547 RNA-Seq mannose Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 11 4 GH130, GH26, GH2, GH3
PUL0565 microarray, qPCR galactomannan, glucomannan Bacteroides ovatus 22205877, 27872187
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus: ENZYME SYNERGY AND CRYSTAL STRUCTURE OF A beta-MANNANASE. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. J Biol Chem. 2017 Jan 6;292(1):229-243. doi: 10.1074/jbc.M116.746438. Epub 2016 Nov 21.
2011 Dec,2017 Jan 6 degradation 10 4 GH130, GH36, GH26, GH26
PUL0600 liquid chromatography and mass spectrometry, differential gene expression galactomannan Clostridium cellulovorans 26020016
Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis. AMB Express. 2015 May 23;5:29. doi: 10.1186/s13568-015-0115-6. eCollection 2015.
2015 degradation 12 2 GH130, GH130, GH2