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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0223

PubMed

23943617, J Biol Chem. 2013 Sep 20;288(38):27366-27374. doi: 10.1074/jbc.M113.469080. Epub 2013 Aug 13.

Characterization method

enzyme specificity assay,enzyme activity assay

Genomic accession number

AE015928.1

Nucelotide position range

1300094-1315194

Substrate

N-glycan

Loci

BT_1032-BT_1040

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_1032 AAO76139.1 0 - 2271 (-) AE015928.1:1300094-1302365 -
- BT_1033 AAO76140.1 2278 - 3247 (-) AE015928.1:1302372-1303341 -
- BT_1034 AAO76141.1 3299 - 4631 (-) AE015928.1:1303393-1304725 -
- BT_1035 AAO76142.1 4729 - 6934 (-) AE015928.1:1304823-1307028 -
- BT_1036 AAO76143.1 7100 - 8450 (-) AE015928.1:1307194-1308544 -
- BT_1037 AAO76144.1 8466 - 9654 (-) AE015928.1:1308560-1309748 -
- BT_1038 AAO76145.1 9697 - 10720 (-) AE015928.1:1309791-1310814 -
- BT_1039 AAO76146.1 10746 - 12330 (-) AE015928.1:1310840-1312424 -
- BT_1040 AAO76147.1 12338 - 15101 (-) AE015928.1:1312432-1315195 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2271 (-) CAZyme: GH92 Yes
- 2279 - 3247 (-) CAZyme: GH130 Yes
- 3300 - 4631 (-) other Yes
- 4730 - 6934 (-) CAZyme: GH163 Yes
- 7101 - 8450 (-) other Yes
- 8467 - 9654 (-) other Yes
- 9698 - 10720 (-) other Yes
- 10747 - 12330 (-) TC: gnl|TC-DB|Q8A8X0|8.A.46.2.1 Yes
- 12339 - 15101 (-) TC: gnl|TC-DB|Q8A8X1|1.B.14.6.13 Yes

PUL ID

PUL0223

PubMed

23943617, J Biol Chem. 2013 Sep 20;288(38):27366-27374. doi: 10.1074/jbc.M113.469080. Epub 2013 Aug 13.

Title

Discovery of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans.

Author

Nihira T, Suzuki E, Kitaoka M, Nishimoto M, Ohtsubo K, Nakai H

Abstract

A gene cluster involved in N-glycan metabolism was identified in the genome of Bacteroides thetaiotaomicron VPI-5482. This gene cluster encodes a major facilitator superfamily transporter, a starch utilization system-like transporter consisting of a TonB-dependent oligosaccharide transporter and an outer membrane lipoprotein, four glycoside hydrolases (alpha-mannosidase, beta-N-acetylhexosaminidase, exo-alpha-sialidase, and endo-beta-N-acetylglucosaminidase), and a phosphorylase (BT1033) with unknown function. It was demonstrated that BT1033 catalyzed the reversible phosphorolysis of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine in a typical sequential Bi Bi mechanism. These results indicate that BT1033 plays a crucial role as a key enzyme in the N-glycan catabolism where beta-1,4-D-mannosyl-N-acetyl-D-glucosamine is liberated from N-glycans by sequential glycoside hydrolase-catalyzed reactions, transported into the cell, and intracellularly converted into alpha-D-mannose 1-phosphate and N-acetyl-D-glucosamine. In addition, intestinal anaerobic bacteria such as Bacteroides fragilis, Bacteroides helcogenes, Bacteroides salanitronis, Bacteroides vulgatus, Prevotella denticola, Prevotella dentalis, Prevotella melaninogenica, Parabacteroides distasonis, and Alistipes finegoldii were also suggested to possess the similar metabolic pathway for N-glycans. A notable feature of the new metabolic pathway for N-glycans is the more efficient use of ATP-stored energy, in comparison with the conventional pathway where beta-mannosidase and ATP-dependent hexokinase participate, because it is possible to directly phosphorylate the D-mannose residue of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine to enter glycolysis. This is the first report of a metabolic pathway for N-glycans that includes a phosphorylase. We propose 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine:phosphate alpha-D-mannosyltransferase as the systematic name and beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase as the short name for BT1033.