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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0175

PubMed

16842369, FEMS Microbiol Lett. 2006 Aug;261(1):123-32. doi: 10.1111/j.1574-6968.2006.00342.x.

Characterization method

enzyme activity assay

Genomic accession number

AY526725.1

Nucelotide position range

111-5257

Substrate

galactomannan

Loci

unkA-epiA-aga27A

Species

Cellvibrio mixtus/39650

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

unk1 - AAS19693.1 111 - 1293 (+) AY526725.1:222-1404 -
unk2 - AAS19694.1 1296 - 2529 (+) AY526725.1:1407-2640 -
man5A - AAS19695.1 2541 - 3912 (+) AY526725.1:2652-4023 -
aga27A - AAS19696.1 4039 - 5257 (+) AY526725.1:4150-5368 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 112 - 1293 (+) CAZyme: GH130 No
- 1297 - 2529 (+) CDS No
- 2542 - 3912 (+) CAZyme: GH5_7|GH5 No
- 4040 - 5257 (+) CAZyme: GH27 No

PUL ID

PUL0175

PubMed

16842369, FEMS Microbiol Lett. 2006 Aug;261(1):123-32. doi: 10.1111/j.1574-6968.2006.00342.x.

Title

Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus.

Author

Centeno MS, Guerreiro CI, Dias FM, Morland C, Tailford LE, Goyal A, Prates JA, Ferreira LM, Caldeira RM, Mongodin EF, Nelson KE, Gilbert HJ, Fontes CM

Abstract

Galactomannan hydrolysis results from the concerted action of microbial endo-mannanases, manosidases and alpha-galactosidases and is a mechanism of intrinsic biological importance. Here we report the identification of a gene cluster in the aerobic soil bacterium Cellvibrio mixtus encoding enzymes involved in the degradation of this polymeric substrate. The family 27 alpha-galactosidase, termed CmAga27A, preferentially hydrolyse galactose containing polysaccharides. In addition, we have characterized an enzyme with epimerase activity, which might be responsible for the conversion of mannose into glucose. The role of the identified enzymes in the hydrolysis of galactomannan by aerobic bacteria is discussed.