dbCAN-PUL

PUL ID	PUL0408
PubMed	24217874, Arch Microbiol. 2014 Jan;196(1):17-23. doi: 10.1007/s00203-013-0938-y. Epub 2013 Nov 12.
Characterization method	enzyme activity assay,thin layer chromatography
Genomic accession number	CR626927.1
Nucelotide position range	944105-949034
Substrate	beta-mannan
Loci	BF9343_0736-BF9343_0739
Species	Bacteroides fragilis/817
Degradation or Biosynthesis	degradation

PUL ID	PUL0408
PubMed	24217874, Arch Microbiol. 2014 Jan;196(1):17-23. doi: 10.1007/s00203-013-0938-y. Epub 2013 Nov 12.
Title	The mannobiose-forming exo-mannanase involved in a new mannan catabolic pathway in Bacteroides fragilis.
Author	Kawaguchi K, Senoura T, Ito S, Taira T, Ito H, Wasaki J, Ito S
Abstract	We have proposed a new mannan catabolic pathway in Bacteroides fragilis NCTC 9343 that involves a putative mannanase ManA in glycoside hydrolase family 26 (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772). If this hypothesis is correct, ManA has to generate mannobiose from mannans as the major end product. In this study, the BF0771 gene from the B. fragilis genome was cloned and expressed in Escherichia coli cells. The expressed protein was found to produce mannobiose exclusively from mannans and initially from manno-oligosaccharides. Production of 4-O-beta-D-glucopyranosyl-D-mannose or 4-O-beta-D-mannopyranosyl-D-glucose from mannans was not detectable. The results indicate that this enzyme is a novel mannobiose-forming exo-mannanase, consistent with the new microbial mannan catabolic pathway we proposed.