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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0165

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Characterization method

mass spectrometry,sequence homology analysis,differential gene expression

Genomic accession number

JQNQ01000001.1

Nucelotide position range

2251021-2277198

Substrate

beta-mannan

Loci

FG27draft_2042-FG27draft_2057

Species

Salegentibacter sp. Hel_I_6/1250278

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - JQNQ01000001_1 1 - 1818 (-) JQNQ01000001.1:2251022-2252839 -
- - JQNQ01000001_2 1948 - 4428 (-) JQNQ01000001.1:2252969-2255449 -
- - JQNQ01000001_3 4588 - 5961 (+) JQNQ01000001.1:2255609-2256982 -
- - JQNQ01000001_4 6026 - 7231 (-) JQNQ01000001.1:2257047-2258252 -
- - JQNQ01000001_5 7317 - 9803 (-) JQNQ01000001.1:2258338-2260824 -
- - JQNQ01000001_6 9931 - 11010 (-) JQNQ01000001.1:2260952-2262031 -
- - JQNQ01000001_7 11033 - 12610 (-) JQNQ01000001.1:2262054-2263631 -
- - JQNQ01000001_8 12622 - 15792 (-) JQNQ01000001.1:2263643-2266813 -
- - JQNQ01000001_9 16102 - 17085 (-) JQNQ01000001.1:2267123-2268106 -
- - JQNQ01000001_10 17098 - 17970 (-) JQNQ01000001.1:2268119-2268991 -
- - JQNQ01000001_11 18179 - 19300 (+) JQNQ01000001.1:2269200-2270321 -
- - JQNQ01000001_12 19476 - 21152 (+) JQNQ01000001.1:2270497-2272173 -
- - JQNQ01000001_13 21162 - 22352 (+) JQNQ01000001.1:2272183-2273373 -
- - JQNQ01000001_14 22353 - 23546 (+) JQNQ01000001.1:2273374-2274567 -
- - JQNQ01000001_15 23543 - 24805 (+) JQNQ01000001.1:2274564-2275826 -
- - JQNQ01000001_16 24802 - 26178 (-) JQNQ01000001.1:2275823-2277199 -
- - JQNQ01000001_17 26123 - 26194 (-) JQNQ01000001.1:2277144-2277215 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1818 (-) CDS No
- 1948 - 4428 (-) CAZyme: GH9 Yes
- 4588 - 5961 (+) other Yes
- 6026 - 7231 (-) CAZyme: GH27 Yes
- 7317 - 9803 (-) other Yes
- 9931 - 11010 (-) other Yes
- 11033 - 12610 (-) TC: gnl|TC-DB|G0IYY9|8.A.46.1.6 Yes
- 12622 - 15792 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 16102 - 17085 (-) CAZyme: GH5|GH5_2 Yes
- 17098 - 17970 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607,DBD-SUPERFAMILY|0035607 Yes
- 18179 - 19300 (+) CAZyme: GH26 Yes
- 19476 - 21152 (+) TC: gnl|TC-DB|A1S2A8|2.A.21.3.8 Yes
- 21162 - 22352 (+) CAZyme: GH130 Yes
- 22353 - 23546 (+) other Yes
- 23543 - 24805 (+) CAZyme: GH26 Yes
- 24802 - 26178 (-) CDS No
- 26123 - 26194 (-) CDS No

PUL ID

PUL0165

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Title

Alpha- and beta-mannan utilization by marine Bacteroidetes.

Author

Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avci B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H

Abstract

Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest alpha- and beta-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for alpha- and beta-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.