PUL ID

PUL0163

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Characterization method

mass spectrometry, sequence homology analysis

Genomic accession number

NC_004663.1

Nucelotide position range

4893415-4930042

Substrate

alpha-mannan

Loci

BT_3773-BT_3793

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_RS19030 WP_011108964.1 0 - 2262 (+) NC_004663.1:4893415-4895677 -
- BT_RS19035 WP_011108965.1 2526 - 6123 (-) NC_004663.1:4895941-4899538 -
- BT_RS19040 WP_008766953.1 6718 - 7393 (-) NC_004663.1:4900133-4900808 -
- BT_RS19045 WP_008766952.1 7414 - 8116 (-) NC_004663.1:4900829-4901531 -
- BT_RS19050 WP_008766951.1 8121 - 8922 (-) NC_004663.1:4901536-4902337 -
- BT_RS19055 WP_008766950.1 9005 - 9950 (-) NC_004663.1:4902420-4903365 -
- BT_RS19060 WP_008762716.1 10033 - 11455 (-) NC_004663.1:4903448-4904870 -
- BT_RS19065 WP_008762717.1 11702 - 12854 (-) NC_004663.1:4905117-4906269 -
- BT_RS19070 WP_011108966.1 13304 - 14750 (-) NC_004663.1:4906719-4908165 -
- BT_RS19075 WP_011108967.1 14795 - 15959 (-) NC_004663.1:4908210-4909374 -
- BT_RS19080 WP_011108968.1 15993 - 16941 (-) NC_004663.1:4909408-4910356 -
- BT_RS19085 WP_008762721.1 16999 - 19282 (-) NC_004663.1:4910414-4912697 -
- BT_RS19090 WP_011108970.1 19491 - 23529 (+) NC_004663.1:4912906-4916944 -
- BT_RS19095 WP_011108971.1 24047 - 25406 (+) NC_004663.1:4917462-4918821 -
- BT_RS19100 WP_011108972.1 25428 - 28530 (+) NC_004663.1:4918843-4921945 -
- BT_RS19105 WP_011108973.1 28549 - 30529 (+) NC_004663.1:4921964-4923944 -
- BT_RS19110 WP_011108974.1 30547 - 31522 (+) NC_004663.1:4923962-4924937 -
- BT_RS19115 WP_162303170.1 31601 - 33230 (+) NC_004663.1:4925016-4926645 -
- BT_RS19120 WP_011108976.1 33249 - 34827 (+) NC_004663.1:4926664-4928242 -
- BT_RS19125 WP_011108977.1 34918 - 35812 (-) NC_004663.1:4928333-4929227 -
- BT_RS19130 WP_008762730.1 36426 - 36628 (-) NC_004663.1:4929841-4930043 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2262 (+) CAZyme: GH92 Yes
- 2527 - 6123 (-) CAZyme: GH38|CBM32 Yes
- 6719 - 7393 (-) CAZyme: GT32 Yes
- 7415 - 8116 (-) CAZyme: GT32 Yes
- 8122 - 8922 (-) other Yes
- 9006 - 9950 (-) other Yes
- 10034 - 11455 (-) other Yes
- 11703 - 12854 (-) CAZyme: GH130 Yes
- 13305 - 14750 (-) CAZyme: GH125 Yes
- 14796 - 15959 (-) CAZyme: GH76 Yes
- 15994 - 16941 (-) other Yes
- 17000 - 19282 (-) CAZyme: GH92 Yes
- 19492 - 23529 (+) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 Yes
- 24048 - 25406 (+) other Yes
- 25429 - 28530 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 28550 - 30529 (+) other Yes
- 30548 - 31522 (+) other Yes
- 31602 - 33230 (+) other Yes
- 33250 - 34827 (+) CAZyme: GH76 Yes
- 34919 - 35812 (-) CDS No
- 36427 - 36628 (-) CDS No

PUL ID

PUL0163

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Title

Alpha- and beta-mannan utilization by marine Bacteroidetes.

Author

Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avci B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H

Abstract

Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest alpha- and beta-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for alpha- and beta-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.